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Double-layered cell transfer technology for bone regeneration

For cell-based medicine, to mimic in vivo cellular localization, various tissue engineering approaches have been studied to obtain a desirable arrangement of cells on scaffold materials. We have developed a novel method of cell manipulation called “cell transfer technology”, enabling the transfer of...

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Autores principales: Akazawa, Keiko, Iwasaki, Kengo, Nagata, Mizuki, Yokoyama, Naoki, Ayame, Hirohito, Yamaki, Kazumasa, Tanaka, Yuichi, Honda, Izumi, Morioka, Chikako, Kimura, Tsuyoshi, Komaki, Motohiro, Kishida, Akio, Izumi, Yuichi, Morita, Ikuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5021950/
https://www.ncbi.nlm.nih.gov/pubmed/27624174
http://dx.doi.org/10.1038/srep33286
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author Akazawa, Keiko
Iwasaki, Kengo
Nagata, Mizuki
Yokoyama, Naoki
Ayame, Hirohito
Yamaki, Kazumasa
Tanaka, Yuichi
Honda, Izumi
Morioka, Chikako
Kimura, Tsuyoshi
Komaki, Motohiro
Kishida, Akio
Izumi, Yuichi
Morita, Ikuo
author_facet Akazawa, Keiko
Iwasaki, Kengo
Nagata, Mizuki
Yokoyama, Naoki
Ayame, Hirohito
Yamaki, Kazumasa
Tanaka, Yuichi
Honda, Izumi
Morioka, Chikako
Kimura, Tsuyoshi
Komaki, Motohiro
Kishida, Akio
Izumi, Yuichi
Morita, Ikuo
author_sort Akazawa, Keiko
collection PubMed
description For cell-based medicine, to mimic in vivo cellular localization, various tissue engineering approaches have been studied to obtain a desirable arrangement of cells on scaffold materials. We have developed a novel method of cell manipulation called “cell transfer technology”, enabling the transfer of cultured cells onto scaffold materials, and controlling cell topology. Here we show that using this technique, two different cell types can be transferred onto a scaffold surface as stable double layers or in patterned arrangements. Various combinations of adherent cells were transferred to a scaffold, amniotic membrane, in overlapping bilayers (double-layered cell transfer), and transferred cells showed stability upon deformations of the material including folding and trimming. Transplantation of mesenchymal stem cells from periodontal ligaments (PDLSC) and osteoblasts, using double-layered cell transfer significantly enhanced bone formation, when compared to single cell type transplantation. Our findings suggest that this double-layer cell transfer is useful to produce a cell transplantation material that can bear two cell layers. Moreover, the transplantation of an amniotic membrane with PDLSCs/osteoblasts by cell transfer technology has therapeutic potential for bone defects. We conclude that cell transfer technology provides a novel and unique cell transplantation method for bone regeneration.
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spelling pubmed-50219502016-09-20 Double-layered cell transfer technology for bone regeneration Akazawa, Keiko Iwasaki, Kengo Nagata, Mizuki Yokoyama, Naoki Ayame, Hirohito Yamaki, Kazumasa Tanaka, Yuichi Honda, Izumi Morioka, Chikako Kimura, Tsuyoshi Komaki, Motohiro Kishida, Akio Izumi, Yuichi Morita, Ikuo Sci Rep Article For cell-based medicine, to mimic in vivo cellular localization, various tissue engineering approaches have been studied to obtain a desirable arrangement of cells on scaffold materials. We have developed a novel method of cell manipulation called “cell transfer technology”, enabling the transfer of cultured cells onto scaffold materials, and controlling cell topology. Here we show that using this technique, two different cell types can be transferred onto a scaffold surface as stable double layers or in patterned arrangements. Various combinations of adherent cells were transferred to a scaffold, amniotic membrane, in overlapping bilayers (double-layered cell transfer), and transferred cells showed stability upon deformations of the material including folding and trimming. Transplantation of mesenchymal stem cells from periodontal ligaments (PDLSC) and osteoblasts, using double-layered cell transfer significantly enhanced bone formation, when compared to single cell type transplantation. Our findings suggest that this double-layer cell transfer is useful to produce a cell transplantation material that can bear two cell layers. Moreover, the transplantation of an amniotic membrane with PDLSCs/osteoblasts by cell transfer technology has therapeutic potential for bone defects. We conclude that cell transfer technology provides a novel and unique cell transplantation method for bone regeneration. Nature Publishing Group 2016-09-14 /pmc/articles/PMC5021950/ /pubmed/27624174 http://dx.doi.org/10.1038/srep33286 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Akazawa, Keiko
Iwasaki, Kengo
Nagata, Mizuki
Yokoyama, Naoki
Ayame, Hirohito
Yamaki, Kazumasa
Tanaka, Yuichi
Honda, Izumi
Morioka, Chikako
Kimura, Tsuyoshi
Komaki, Motohiro
Kishida, Akio
Izumi, Yuichi
Morita, Ikuo
Double-layered cell transfer technology for bone regeneration
title Double-layered cell transfer technology for bone regeneration
title_full Double-layered cell transfer technology for bone regeneration
title_fullStr Double-layered cell transfer technology for bone regeneration
title_full_unstemmed Double-layered cell transfer technology for bone regeneration
title_short Double-layered cell transfer technology for bone regeneration
title_sort double-layered cell transfer technology for bone regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5021950/
https://www.ncbi.nlm.nih.gov/pubmed/27624174
http://dx.doi.org/10.1038/srep33286
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