Cargando…
Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin
OBJECTIVE: Green tea extract has been advocated as a matrix metalloproteinase (MMP) inhibitor; however, its effect on bond durability to caries-affected dentin has never been reported. Thus, the aim of this in vitro study was to evaluate the effect of two MMP inhibitors (2% chlorhexidine and 2% gree...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Faculdade De Odontologia De Bauru - USP
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5022223/ https://www.ncbi.nlm.nih.gov/pubmed/27383701 http://dx.doi.org/10.1590/1678-775720150518 |
_version_ | 1782453481507913728 |
---|---|
author | CARVALHO, Carolina FERNANDES, Fernando Pelegrim FREITAS, Valeria da Penha FRANÇA, Fabiana Mantovani Gomes BASTING, Roberta Tarkany TURSSI, Cecilia Pedroso AMARAL, Flávia Lucisano Botelho |
author_facet | CARVALHO, Carolina FERNANDES, Fernando Pelegrim FREITAS, Valeria da Penha FRANÇA, Fabiana Mantovani Gomes BASTING, Roberta Tarkany TURSSI, Cecilia Pedroso AMARAL, Flávia Lucisano Botelho |
author_sort | CARVALHO, Carolina |
collection | PubMed |
description | OBJECTIVE: Green tea extract has been advocated as a matrix metalloproteinase (MMP) inhibitor; however, its effect on bond durability to caries-affected dentin has never been reported. Thus, the aim of this in vitro study was to evaluate the effect of two MMP inhibitors (2% chlorhexidine and 2% green tea extract), applied after acid etching, on bond durability of an etch-and-rinse adhesive system to caries-affected dentin. MATERIAL AND METHODS: Occlusal enamel was removed from third molars to expose the dentin surface, and the molars were submitted to a caries induction protocol for 15 days. After removal of infected dentin, specimens were conditioned with 37% phosphoric acid (15 seconds) and randomly divided into three groups, according to the type of dentin pretreatment (n=10): NT: no treatment; GT: 2% green tea extract; CLX: 2% chlorhexidine. The etch-and-rinse adhesive system (Adper™ Single Bond 2, 3M ESPE, St. Paul, MN, USA) was applied according to the manufacturer's instructions, and composite resin restorations were built on the dentin. After 24 hours, at 37°C, the resin-tooth blocks were sectioned perpendicularly to the adhesive interface in the form of sticks (0.8 mm(2) of adhesive area) and randomly subdivided into two groups according to when they were to be submitted to microtensile bond strength (μTBS) testing: immediately or 6 months after storage in distilled water. Data were reported in MPa and submitted to two-way ANOVA for completely randomized blocks, followed by Tukey’s test (α=0.05). RESULTS: After 24 hours, there was no significant difference in the μTBS of the groups. After 6 months, the GT group had significantly higher μTBS values. CONCLUSION: It was concluded that the application of 2% green tea extract was able to increase bond durability of the etch-and-rinse system to dentin. Neither the application of chlorhexidine nor non-treatment (NT - control) had any effect on bond strength after water storage. |
format | Online Article Text |
id | pubmed-5022223 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Faculdade De Odontologia De Bauru - USP |
record_format | MEDLINE/PubMed |
spelling | pubmed-50222232016-09-15 Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin CARVALHO, Carolina FERNANDES, Fernando Pelegrim FREITAS, Valeria da Penha FRANÇA, Fabiana Mantovani Gomes BASTING, Roberta Tarkany TURSSI, Cecilia Pedroso AMARAL, Flávia Lucisano Botelho J Appl Oral Sci Original Articles OBJECTIVE: Green tea extract has been advocated as a matrix metalloproteinase (MMP) inhibitor; however, its effect on bond durability to caries-affected dentin has never been reported. Thus, the aim of this in vitro study was to evaluate the effect of two MMP inhibitors (2% chlorhexidine and 2% green tea extract), applied after acid etching, on bond durability of an etch-and-rinse adhesive system to caries-affected dentin. MATERIAL AND METHODS: Occlusal enamel was removed from third molars to expose the dentin surface, and the molars were submitted to a caries induction protocol for 15 days. After removal of infected dentin, specimens were conditioned with 37% phosphoric acid (15 seconds) and randomly divided into three groups, according to the type of dentin pretreatment (n=10): NT: no treatment; GT: 2% green tea extract; CLX: 2% chlorhexidine. The etch-and-rinse adhesive system (Adper™ Single Bond 2, 3M ESPE, St. Paul, MN, USA) was applied according to the manufacturer's instructions, and composite resin restorations were built on the dentin. After 24 hours, at 37°C, the resin-tooth blocks were sectioned perpendicularly to the adhesive interface in the form of sticks (0.8 mm(2) of adhesive area) and randomly subdivided into two groups according to when they were to be submitted to microtensile bond strength (μTBS) testing: immediately or 6 months after storage in distilled water. Data were reported in MPa and submitted to two-way ANOVA for completely randomized blocks, followed by Tukey’s test (α=0.05). RESULTS: After 24 hours, there was no significant difference in the μTBS of the groups. After 6 months, the GT group had significantly higher μTBS values. CONCLUSION: It was concluded that the application of 2% green tea extract was able to increase bond durability of the etch-and-rinse system to dentin. Neither the application of chlorhexidine nor non-treatment (NT - control) had any effect on bond strength after water storage. Faculdade De Odontologia De Bauru - USP 2016 /pmc/articles/PMC5022223/ /pubmed/27383701 http://dx.doi.org/10.1590/1678-775720150518 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles CARVALHO, Carolina FERNANDES, Fernando Pelegrim FREITAS, Valeria da Penha FRANÇA, Fabiana Mantovani Gomes BASTING, Roberta Tarkany TURSSI, Cecilia Pedroso AMARAL, Flávia Lucisano Botelho Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin |
title | Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin |
title_full | Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin |
title_fullStr | Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin |
title_full_unstemmed | Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin |
title_short | Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin |
title_sort | effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5022223/ https://www.ncbi.nlm.nih.gov/pubmed/27383701 http://dx.doi.org/10.1590/1678-775720150518 |
work_keys_str_mv | AT carvalhocarolina effectofgreenteaextractonbondingdurabilityofanetchandrinseadhesivesystemtocariesaffecteddentin AT fernandesfernandopelegrim effectofgreenteaextractonbondingdurabilityofanetchandrinseadhesivesystemtocariesaffecteddentin AT freitasvaleriadapenha effectofgreenteaextractonbondingdurabilityofanetchandrinseadhesivesystemtocariesaffecteddentin AT francafabianamantovanigomes effectofgreenteaextractonbondingdurabilityofanetchandrinseadhesivesystemtocariesaffecteddentin AT bastingrobertatarkany effectofgreenteaextractonbondingdurabilityofanetchandrinseadhesivesystemtocariesaffecteddentin AT turssiceciliapedroso effectofgreenteaextractonbondingdurabilityofanetchandrinseadhesivesystemtocariesaffecteddentin AT amaralflavialucisanobotelho effectofgreenteaextractonbondingdurabilityofanetchandrinseadhesivesystemtocariesaffecteddentin |