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Assessment of micronuclei frequency in individuals with a habit of tobacco by means of exfoliated oral buccal cells

AIMS AND OBJECTIVES: To study the genotoxic effects of tobacco on the exfoliated buccal epithelial cells in patients with oral precancerous lesions (OPLs) and Patients with tobacco habit but without oral precancerous lesion(habit controls) by using micronucleus assay as well as the quantification an...

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Autores principales: Dosi, Tanvi, Gupta, Dhaman, Hazari, Alka, Rajput, Rajan, Chauhan, Prabhav, Rajapuri, Anushri S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5022392/
https://www.ncbi.nlm.nih.gov/pubmed/27652247
http://dx.doi.org/10.4103/2231-0762.189745
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author Dosi, Tanvi
Gupta, Dhaman
Hazari, Alka
Rajput, Rajan
Chauhan, Prabhav
Rajapuri, Anushri S.
author_facet Dosi, Tanvi
Gupta, Dhaman
Hazari, Alka
Rajput, Rajan
Chauhan, Prabhav
Rajapuri, Anushri S.
author_sort Dosi, Tanvi
collection PubMed
description AIMS AND OBJECTIVES: To study the genotoxic effects of tobacco on the exfoliated buccal epithelial cells in patients with oral precancerous lesions (OPLs) and Patients with tobacco habit but without oral precancerous lesion(habit controls) by using micronucleus assay as well as the quantification and detection of the biomarkers in these premalignant lesions which will be helpful in finding those patients who are at higher risk for malignant transformation. MATERIALS AND METHODS: Forty samples were collected from the right and left side of buccal epithelial cells obtained from 20 individuals, i.e., 10 patients with habit control and 10 patients with OPLs. Statistical analysis was performed by the Statistical Package for the Social Sciences version 21.0 Unpaired t-test was performed to determine the micronucleated cell (MNC) and micronuclei (MN) frequencies in individuals; significance was set at P > 0.05. RESULTS: There was an increase in both the MNC and MN frequency from habit controls to OPLs, indicating that the number of cells with chromosomal damage and extent of chromosomal damage in each cell was high in OPLs. CONCLUSION: The MN count can be used as a noninvasive tool for early detection, educating patients, screening a large population, and to check the risk for malignancy, which in turn may help in treatment planning.
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spelling pubmed-50223922016-09-20 Assessment of micronuclei frequency in individuals with a habit of tobacco by means of exfoliated oral buccal cells Dosi, Tanvi Gupta, Dhaman Hazari, Alka Rajput, Rajan Chauhan, Prabhav Rajapuri, Anushri S. J Int Soc Prev Community Dent Original Article AIMS AND OBJECTIVES: To study the genotoxic effects of tobacco on the exfoliated buccal epithelial cells in patients with oral precancerous lesions (OPLs) and Patients with tobacco habit but without oral precancerous lesion(habit controls) by using micronucleus assay as well as the quantification and detection of the biomarkers in these premalignant lesions which will be helpful in finding those patients who are at higher risk for malignant transformation. MATERIALS AND METHODS: Forty samples were collected from the right and left side of buccal epithelial cells obtained from 20 individuals, i.e., 10 patients with habit control and 10 patients with OPLs. Statistical analysis was performed by the Statistical Package for the Social Sciences version 21.0 Unpaired t-test was performed to determine the micronucleated cell (MNC) and micronuclei (MN) frequencies in individuals; significance was set at P > 0.05. RESULTS: There was an increase in both the MNC and MN frequency from habit controls to OPLs, indicating that the number of cells with chromosomal damage and extent of chromosomal damage in each cell was high in OPLs. CONCLUSION: The MN count can be used as a noninvasive tool for early detection, educating patients, screening a large population, and to check the risk for malignancy, which in turn may help in treatment planning. Medknow Publications & Media Pvt Ltd 2016-08 /pmc/articles/PMC5022392/ /pubmed/27652247 http://dx.doi.org/10.4103/2231-0762.189745 Text en Copyright: © Journal of International Society of Preventive and Community Dentistry http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Dosi, Tanvi
Gupta, Dhaman
Hazari, Alka
Rajput, Rajan
Chauhan, Prabhav
Rajapuri, Anushri S.
Assessment of micronuclei frequency in individuals with a habit of tobacco by means of exfoliated oral buccal cells
title Assessment of micronuclei frequency in individuals with a habit of tobacco by means of exfoliated oral buccal cells
title_full Assessment of micronuclei frequency in individuals with a habit of tobacco by means of exfoliated oral buccal cells
title_fullStr Assessment of micronuclei frequency in individuals with a habit of tobacco by means of exfoliated oral buccal cells
title_full_unstemmed Assessment of micronuclei frequency in individuals with a habit of tobacco by means of exfoliated oral buccal cells
title_short Assessment of micronuclei frequency in individuals with a habit of tobacco by means of exfoliated oral buccal cells
title_sort assessment of micronuclei frequency in individuals with a habit of tobacco by means of exfoliated oral buccal cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5022392/
https://www.ncbi.nlm.nih.gov/pubmed/27652247
http://dx.doi.org/10.4103/2231-0762.189745
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