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QTL analysis of soft scald in two apple populations
The apple (Malus×domestica Borkh.) is one of the world’s most widely grown and valuable fruit crops. With demand for apples year round, storability has emerged as an important consideration for apple breeding programs. Soft scald is a cold storage-related disorder that results in sunken, darkened ti...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5022660/ https://www.ncbi.nlm.nih.gov/pubmed/27651916 http://dx.doi.org/10.1038/hortres.2016.43 |
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author | McClure, Kendra A Gardner, Kyle M Toivonen, Peter MA Hampson, Cheryl R Song, Jun Forney, Charles F DeLong, John Rajcan, Istvan Myles, Sean |
author_facet | McClure, Kendra A Gardner, Kyle M Toivonen, Peter MA Hampson, Cheryl R Song, Jun Forney, Charles F DeLong, John Rajcan, Istvan Myles, Sean |
author_sort | McClure, Kendra A |
collection | PubMed |
description | The apple (Malus×domestica Borkh.) is one of the world’s most widely grown and valuable fruit crops. With demand for apples year round, storability has emerged as an important consideration for apple breeding programs. Soft scald is a cold storage-related disorder that results in sunken, darkened tissue on the fruit surface. Apple breeders are keen to generate new cultivars that do not suffer from soft scald and can thus be marketed year round. Traditional breeding approaches are protracted and labor intensive, and therefore marker-assisted selection (MAS) is a valuable tool for breeders. To advance MAS for storage disorders in apple, we used genotyping-by-sequencing (GBS) to generate high-density genetic maps in two F(1) apple populations, which were then used for quantitative trait locus (QTL) mapping of soft scald. In total, 900 million DNA sequence reads were generated, but after several data filtering steps, only 2% of reads were ultimately used to create two genetic maps that included 1918 and 2818 single-nucleotide polymorphisms. Two QTL associated with soft scald were identified in one of the bi-parental populations originating from parent 11W-12-11, an advanced breeding line. This study demonstrates the utility of next-generation DNA sequencing technologies for QTL mapping in F(1) populations, and provides a basis for the advancement of MAS to improve storability of apples. |
format | Online Article Text |
id | pubmed-5022660 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50226602016-09-20 QTL analysis of soft scald in two apple populations McClure, Kendra A Gardner, Kyle M Toivonen, Peter MA Hampson, Cheryl R Song, Jun Forney, Charles F DeLong, John Rajcan, Istvan Myles, Sean Hortic Res Article The apple (Malus×domestica Borkh.) is one of the world’s most widely grown and valuable fruit crops. With demand for apples year round, storability has emerged as an important consideration for apple breeding programs. Soft scald is a cold storage-related disorder that results in sunken, darkened tissue on the fruit surface. Apple breeders are keen to generate new cultivars that do not suffer from soft scald and can thus be marketed year round. Traditional breeding approaches are protracted and labor intensive, and therefore marker-assisted selection (MAS) is a valuable tool for breeders. To advance MAS for storage disorders in apple, we used genotyping-by-sequencing (GBS) to generate high-density genetic maps in two F(1) apple populations, which were then used for quantitative trait locus (QTL) mapping of soft scald. In total, 900 million DNA sequence reads were generated, but after several data filtering steps, only 2% of reads were ultimately used to create two genetic maps that included 1918 and 2818 single-nucleotide polymorphisms. Two QTL associated with soft scald were identified in one of the bi-parental populations originating from parent 11W-12-11, an advanced breeding line. This study demonstrates the utility of next-generation DNA sequencing technologies for QTL mapping in F(1) populations, and provides a basis for the advancement of MAS to improve storability of apples. Nature Publishing Group 2016-09-14 /pmc/articles/PMC5022660/ /pubmed/27651916 http://dx.doi.org/10.1038/hortres.2016.43 Text en Copyright © 2016 The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article McClure, Kendra A Gardner, Kyle M Toivonen, Peter MA Hampson, Cheryl R Song, Jun Forney, Charles F DeLong, John Rajcan, Istvan Myles, Sean QTL analysis of soft scald in two apple populations |
title | QTL analysis of soft scald in two apple populations |
title_full | QTL analysis of soft scald in two apple populations |
title_fullStr | QTL analysis of soft scald in two apple populations |
title_full_unstemmed | QTL analysis of soft scald in two apple populations |
title_short | QTL analysis of soft scald in two apple populations |
title_sort | qtl analysis of soft scald in two apple populations |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5022660/ https://www.ncbi.nlm.nih.gov/pubmed/27651916 http://dx.doi.org/10.1038/hortres.2016.43 |
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