Cargando…
RNAi-mediated HOXD3 knockdown inhibits growth in human RKO cells
Numerous studies have shown that the multifunctional Homeobox-containing (HOX) D3 gene is involved in various physiological and pathological processes. To elucidate the role and mechanism of HOXD3 in colorectal cancer (CRC), we measured its expression in five CRC cell lines. After determining that H...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5022871/ https://www.ncbi.nlm.nih.gov/pubmed/27499213 http://dx.doi.org/10.3892/or.2016.4993 |
_version_ | 1782453591422795776 |
---|---|
author | Chen, Fangjun Sun, Guoping Peng, Jun |
author_facet | Chen, Fangjun Sun, Guoping Peng, Jun |
author_sort | Chen, Fangjun |
collection | PubMed |
description | Numerous studies have shown that the multifunctional Homeobox-containing (HOX) D3 gene is involved in various physiological and pathological processes. To elucidate the role and mechanism of HOXD3 in colorectal cancer (CRC), we measured its expression in five CRC cell lines. After determining that HOXD3 was highly expressed in the human RKO cancer cell line, we used lentiviral-mediated small interfering RNAs (siRNAs) to knock down HOXD3 expression and assessed proliferation, cell cycle distribution, apoptosis and colony formation using cell proliferation, flow cytometric, and colony formation assays. The expression of HOXD3 was strongly suppressed in the RKO cells infected with the lentiviruse expressing an HOXD3 short hairpin RNA (shRNA). The downregulation of HOXD3 expression in RKO cells significantly decreased proliferation and colony formation, and increased apoptosis in vitro, compared to the cells infected with the mock control (p<0.01). Moreover, specific downregulation of HOXD3 led to the accumulation of cells at the G(2) phase of the cell cycle. Our findings revealed that the HOXD3 gene promotes CRC cell growth and plays a pivotal role in the development and survival of malignant human colorectal cancer cells. |
format | Online Article Text |
id | pubmed-5022871 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-50228712016-09-22 RNAi-mediated HOXD3 knockdown inhibits growth in human RKO cells Chen, Fangjun Sun, Guoping Peng, Jun Oncol Rep Articles Numerous studies have shown that the multifunctional Homeobox-containing (HOX) D3 gene is involved in various physiological and pathological processes. To elucidate the role and mechanism of HOXD3 in colorectal cancer (CRC), we measured its expression in five CRC cell lines. After determining that HOXD3 was highly expressed in the human RKO cancer cell line, we used lentiviral-mediated small interfering RNAs (siRNAs) to knock down HOXD3 expression and assessed proliferation, cell cycle distribution, apoptosis and colony formation using cell proliferation, flow cytometric, and colony formation assays. The expression of HOXD3 was strongly suppressed in the RKO cells infected with the lentiviruse expressing an HOXD3 short hairpin RNA (shRNA). The downregulation of HOXD3 expression in RKO cells significantly decreased proliferation and colony formation, and increased apoptosis in vitro, compared to the cells infected with the mock control (p<0.01). Moreover, specific downregulation of HOXD3 led to the accumulation of cells at the G(2) phase of the cell cycle. Our findings revealed that the HOXD3 gene promotes CRC cell growth and plays a pivotal role in the development and survival of malignant human colorectal cancer cells. D.A. Spandidos 2016-10 2016-08-02 /pmc/articles/PMC5022871/ /pubmed/27499213 http://dx.doi.org/10.3892/or.2016.4993 Text en Copyright: © Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Chen, Fangjun Sun, Guoping Peng, Jun RNAi-mediated HOXD3 knockdown inhibits growth in human RKO cells |
title | RNAi-mediated HOXD3 knockdown inhibits growth in human RKO cells |
title_full | RNAi-mediated HOXD3 knockdown inhibits growth in human RKO cells |
title_fullStr | RNAi-mediated HOXD3 knockdown inhibits growth in human RKO cells |
title_full_unstemmed | RNAi-mediated HOXD3 knockdown inhibits growth in human RKO cells |
title_short | RNAi-mediated HOXD3 knockdown inhibits growth in human RKO cells |
title_sort | rnai-mediated hoxd3 knockdown inhibits growth in human rko cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5022871/ https://www.ncbi.nlm.nih.gov/pubmed/27499213 http://dx.doi.org/10.3892/or.2016.4993 |
work_keys_str_mv | AT chenfangjun rnaimediatedhoxd3knockdowninhibitsgrowthinhumanrkocells AT sunguoping rnaimediatedhoxd3knockdowninhibitsgrowthinhumanrkocells AT pengjun rnaimediatedhoxd3knockdowninhibitsgrowthinhumanrkocells |