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Expression pattern and methylation of estrogen receptor α in breast intraductal proliferative lesions
Intraductal proliferative lesions of the breast including usual ductal hyperplasia (UDH), atypical ductal hyperplasia (ADH) and ductal carcinoma in situ (DCIS) are associated with increased risk, albeit of greatly different magnitudes, for the subsequent development of invasive carcinoma. Estrogen r...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5022874/ https://www.ncbi.nlm.nih.gov/pubmed/27498697 http://dx.doi.org/10.3892/or.2016.4988 |
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author | Mao, Xiaoyun Qiao, Zhen Fan, Chuifeng Guo, Ayao Yu, Xinmiao Jin, Feng |
author_facet | Mao, Xiaoyun Qiao, Zhen Fan, Chuifeng Guo, Ayao Yu, Xinmiao Jin, Feng |
author_sort | Mao, Xiaoyun |
collection | PubMed |
description | Intraductal proliferative lesions of the breast including usual ductal hyperplasia (UDH), atypical ductal hyperplasia (ADH) and ductal carcinoma in situ (DCIS) are associated with increased risk, albeit of greatly different magnitudes, for the subsequent development of invasive carcinoma. Estrogen receptor α (ERα) has been widely accepted as a prognostic marker and a predictor for endocrine therapy response of breast cancer. To investigate the ERα expression and methylation in breast intraductal proliferative lesions, we analyzed ERα expression in breast intraductal proliferative lesions including pure UDH (N=98), ADH without DCIS (N=160), DCIS without invasive breast cancer (N=149) by immunohistochemistry. Furthermore, the methylation status of ERα by methylation-specific PCR (MSP) was defined in 217 cases of breast intraductal proliferative lesions. Immunohistochemistry showed that 98/98 (100%) of the UDH cases were positive for ERα expression. ERα protein expression in ADH (132/160) (92.5%) was higher than in DCIS (101/149) (67.8%). But the ERα expression pattern was different with histological diversity of breast intraductal proliferative lesions. The average percent cells staining positive for ERα was 35.33% in UDH, 87.75% in ADH and 71.45% in DCIS. ERα methylation in 32/60 (53.3%) UDH, 11/77 (10.2%) ADH and 32/80 (40.0%) DCIS. Our results demonstrated a strong negative correlation between the percent of cells staining positive for ERα and ERα methylation (r=−0.831, p<0.001). Taken together, our results underlined that ERα expression or methylation may be involved in the breast carcinogenesis and advancement, thus it is not parallel to breast cancer risk in breast intraductal proliferative lesions. No obvious watershed between ERα-positive and -negative breast carcinogenesis was established. Estrogen receptor (ER) methylation or expression is a reversible signal in breast carcinogenesis which affected biological behavior of cells. |
format | Online Article Text |
id | pubmed-5022874 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-50228742016-09-22 Expression pattern and methylation of estrogen receptor α in breast intraductal proliferative lesions Mao, Xiaoyun Qiao, Zhen Fan, Chuifeng Guo, Ayao Yu, Xinmiao Jin, Feng Oncol Rep Articles Intraductal proliferative lesions of the breast including usual ductal hyperplasia (UDH), atypical ductal hyperplasia (ADH) and ductal carcinoma in situ (DCIS) are associated with increased risk, albeit of greatly different magnitudes, for the subsequent development of invasive carcinoma. Estrogen receptor α (ERα) has been widely accepted as a prognostic marker and a predictor for endocrine therapy response of breast cancer. To investigate the ERα expression and methylation in breast intraductal proliferative lesions, we analyzed ERα expression in breast intraductal proliferative lesions including pure UDH (N=98), ADH without DCIS (N=160), DCIS without invasive breast cancer (N=149) by immunohistochemistry. Furthermore, the methylation status of ERα by methylation-specific PCR (MSP) was defined in 217 cases of breast intraductal proliferative lesions. Immunohistochemistry showed that 98/98 (100%) of the UDH cases were positive for ERα expression. ERα protein expression in ADH (132/160) (92.5%) was higher than in DCIS (101/149) (67.8%). But the ERα expression pattern was different with histological diversity of breast intraductal proliferative lesions. The average percent cells staining positive for ERα was 35.33% in UDH, 87.75% in ADH and 71.45% in DCIS. ERα methylation in 32/60 (53.3%) UDH, 11/77 (10.2%) ADH and 32/80 (40.0%) DCIS. Our results demonstrated a strong negative correlation between the percent of cells staining positive for ERα and ERα methylation (r=−0.831, p<0.001). Taken together, our results underlined that ERα expression or methylation may be involved in the breast carcinogenesis and advancement, thus it is not parallel to breast cancer risk in breast intraductal proliferative lesions. No obvious watershed between ERα-positive and -negative breast carcinogenesis was established. Estrogen receptor (ER) methylation or expression is a reversible signal in breast carcinogenesis which affected biological behavior of cells. D.A. Spandidos 2016-10 2016-08-01 /pmc/articles/PMC5022874/ /pubmed/27498697 http://dx.doi.org/10.3892/or.2016.4988 Text en Copyright: © Mao et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Mao, Xiaoyun Qiao, Zhen Fan, Chuifeng Guo, Ayao Yu, Xinmiao Jin, Feng Expression pattern and methylation of estrogen receptor α in breast intraductal proliferative lesions |
title | Expression pattern and methylation of estrogen receptor α in breast intraductal proliferative lesions |
title_full | Expression pattern and methylation of estrogen receptor α in breast intraductal proliferative lesions |
title_fullStr | Expression pattern and methylation of estrogen receptor α in breast intraductal proliferative lesions |
title_full_unstemmed | Expression pattern and methylation of estrogen receptor α in breast intraductal proliferative lesions |
title_short | Expression pattern and methylation of estrogen receptor α in breast intraductal proliferative lesions |
title_sort | expression pattern and methylation of estrogen receptor α in breast intraductal proliferative lesions |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5022874/ https://www.ncbi.nlm.nih.gov/pubmed/27498697 http://dx.doi.org/10.3892/or.2016.4988 |
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