Cargando…

A GeXP-Based Assay for Simultaneous Detection of Multiple Viruses in Hospitalized Children with Community Acquired Pneumonia

The GeXP-based assay has recently been developed for simultaneous detection of multiple pathogens. So far, the application of the GeXP assay to test larger clinical samples has hardly been reported. Community-acquired pneumonia (CAP) is the leading cause of death in children worldwide and a substant...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Le, Zhao, Mengchuan, Shi, Zhongren, Feng, Zhishan, Guo, Weiwei, Yang, Shuo, Liu, Lanping, Li, Guixia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5023126/
https://www.ncbi.nlm.nih.gov/pubmed/27627439
http://dx.doi.org/10.1371/journal.pone.0162411
_version_ 1782453623665459200
author Wang, Le
Zhao, Mengchuan
Shi, Zhongren
Feng, Zhishan
Guo, Weiwei
Yang, Shuo
Liu, Lanping
Li, Guixia
author_facet Wang, Le
Zhao, Mengchuan
Shi, Zhongren
Feng, Zhishan
Guo, Weiwei
Yang, Shuo
Liu, Lanping
Li, Guixia
author_sort Wang, Le
collection PubMed
description The GeXP-based assay has recently been developed for simultaneous detection of multiple pathogens. So far, the application of the GeXP assay to test larger clinical samples has hardly been reported. Community-acquired pneumonia (CAP) is the leading cause of death in children worldwide and a substantial proportion of childhood CAP is caused by viruses. Rapid and accurate diagnosis of virus infection is important for the clinical management of CAP. In this study, we explored the GeXP assay for simultaneous detection of 20 types/subtypes of viruses in hospitalized children with CAP. A total of 1699 nasopharyngeal swabs were prospectively collected and viral nucleic acid was extracted and assayed. Using viral genomic DNA or RNA as template, we showed that at the concentration of 10(4) copies of DNA or RNA of each virus/μl, all 20 target viruses were simultaneously identified by the GeXP assay. Fifteen control microorganisms, in contrast, failed to be amplified by the assay. About 65% of cases tested in this study had viral infection, with patients aged <3 years having a 70% positive rate, significantly higher than that in patients aged > 3 years (40%). The most frequently detected virus was RSV followed by PIV3, HRV, ADV and HBoV. Seasonal distribution analysis revealed that RSV was the most predominant in autumn and winter, while in spring and summer PIV3 and RSV were the most frequently identified with similar positive percentages. One hundred twenty randomly-chosen samples tested by the GeXP assay were re-evaluated by mono-RT-PCR, the results showed 97.5% diagnosis agreement between these 2 methods. Our findings suggest that the GeXP assay could be a valuable diagnostic tool for virus infection in pediatric patients with CAP.
format Online
Article
Text
id pubmed-5023126
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-50231262016-09-27 A GeXP-Based Assay for Simultaneous Detection of Multiple Viruses in Hospitalized Children with Community Acquired Pneumonia Wang, Le Zhao, Mengchuan Shi, Zhongren Feng, Zhishan Guo, Weiwei Yang, Shuo Liu, Lanping Li, Guixia PLoS One Research Article The GeXP-based assay has recently been developed for simultaneous detection of multiple pathogens. So far, the application of the GeXP assay to test larger clinical samples has hardly been reported. Community-acquired pneumonia (CAP) is the leading cause of death in children worldwide and a substantial proportion of childhood CAP is caused by viruses. Rapid and accurate diagnosis of virus infection is important for the clinical management of CAP. In this study, we explored the GeXP assay for simultaneous detection of 20 types/subtypes of viruses in hospitalized children with CAP. A total of 1699 nasopharyngeal swabs were prospectively collected and viral nucleic acid was extracted and assayed. Using viral genomic DNA or RNA as template, we showed that at the concentration of 10(4) copies of DNA or RNA of each virus/μl, all 20 target viruses were simultaneously identified by the GeXP assay. Fifteen control microorganisms, in contrast, failed to be amplified by the assay. About 65% of cases tested in this study had viral infection, with patients aged <3 years having a 70% positive rate, significantly higher than that in patients aged > 3 years (40%). The most frequently detected virus was RSV followed by PIV3, HRV, ADV and HBoV. Seasonal distribution analysis revealed that RSV was the most predominant in autumn and winter, while in spring and summer PIV3 and RSV were the most frequently identified with similar positive percentages. One hundred twenty randomly-chosen samples tested by the GeXP assay were re-evaluated by mono-RT-PCR, the results showed 97.5% diagnosis agreement between these 2 methods. Our findings suggest that the GeXP assay could be a valuable diagnostic tool for virus infection in pediatric patients with CAP. Public Library of Science 2016-09-14 /pmc/articles/PMC5023126/ /pubmed/27627439 http://dx.doi.org/10.1371/journal.pone.0162411 Text en © 2016 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wang, Le
Zhao, Mengchuan
Shi, Zhongren
Feng, Zhishan
Guo, Weiwei
Yang, Shuo
Liu, Lanping
Li, Guixia
A GeXP-Based Assay for Simultaneous Detection of Multiple Viruses in Hospitalized Children with Community Acquired Pneumonia
title A GeXP-Based Assay for Simultaneous Detection of Multiple Viruses in Hospitalized Children with Community Acquired Pneumonia
title_full A GeXP-Based Assay for Simultaneous Detection of Multiple Viruses in Hospitalized Children with Community Acquired Pneumonia
title_fullStr A GeXP-Based Assay for Simultaneous Detection of Multiple Viruses in Hospitalized Children with Community Acquired Pneumonia
title_full_unstemmed A GeXP-Based Assay for Simultaneous Detection of Multiple Viruses in Hospitalized Children with Community Acquired Pneumonia
title_short A GeXP-Based Assay for Simultaneous Detection of Multiple Viruses in Hospitalized Children with Community Acquired Pneumonia
title_sort gexp-based assay for simultaneous detection of multiple viruses in hospitalized children with community acquired pneumonia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5023126/
https://www.ncbi.nlm.nih.gov/pubmed/27627439
http://dx.doi.org/10.1371/journal.pone.0162411
work_keys_str_mv AT wangle agexpbasedassayforsimultaneousdetectionofmultiplevirusesinhospitalizedchildrenwithcommunityacquiredpneumonia
AT zhaomengchuan agexpbasedassayforsimultaneousdetectionofmultiplevirusesinhospitalizedchildrenwithcommunityacquiredpneumonia
AT shizhongren agexpbasedassayforsimultaneousdetectionofmultiplevirusesinhospitalizedchildrenwithcommunityacquiredpneumonia
AT fengzhishan agexpbasedassayforsimultaneousdetectionofmultiplevirusesinhospitalizedchildrenwithcommunityacquiredpneumonia
AT guoweiwei agexpbasedassayforsimultaneousdetectionofmultiplevirusesinhospitalizedchildrenwithcommunityacquiredpneumonia
AT yangshuo agexpbasedassayforsimultaneousdetectionofmultiplevirusesinhospitalizedchildrenwithcommunityacquiredpneumonia
AT liulanping agexpbasedassayforsimultaneousdetectionofmultiplevirusesinhospitalizedchildrenwithcommunityacquiredpneumonia
AT liguixia agexpbasedassayforsimultaneousdetectionofmultiplevirusesinhospitalizedchildrenwithcommunityacquiredpneumonia
AT wangle gexpbasedassayforsimultaneousdetectionofmultiplevirusesinhospitalizedchildrenwithcommunityacquiredpneumonia
AT zhaomengchuan gexpbasedassayforsimultaneousdetectionofmultiplevirusesinhospitalizedchildrenwithcommunityacquiredpneumonia
AT shizhongren gexpbasedassayforsimultaneousdetectionofmultiplevirusesinhospitalizedchildrenwithcommunityacquiredpneumonia
AT fengzhishan gexpbasedassayforsimultaneousdetectionofmultiplevirusesinhospitalizedchildrenwithcommunityacquiredpneumonia
AT guoweiwei gexpbasedassayforsimultaneousdetectionofmultiplevirusesinhospitalizedchildrenwithcommunityacquiredpneumonia
AT yangshuo gexpbasedassayforsimultaneousdetectionofmultiplevirusesinhospitalizedchildrenwithcommunityacquiredpneumonia
AT liulanping gexpbasedassayforsimultaneousdetectionofmultiplevirusesinhospitalizedchildrenwithcommunityacquiredpneumonia
AT liguixia gexpbasedassayforsimultaneousdetectionofmultiplevirusesinhospitalizedchildrenwithcommunityacquiredpneumonia