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Genomics of Methylotrophy in Gram-Positive Methylamine-Utilizing Bacteria

Gram-positive methylotrophic bacteria have been known for a long period of time, some serving as model organisms for characterizing the specific details of methylotrophy pathways/enzymes within this group. However, genome-based knowledge of methylotrophy within this group has been so far limited to...

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Autores principales: McTaggart, Tami L., Beck, David A. C., Setboonsarng, Usanisa, Shapiro, Nicole, Woyke, Tanja, Lidstrom, Mary E., Kalyuzhnaya, Marina G., Chistoserdova, Ludmila
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5023230/
https://www.ncbi.nlm.nih.gov/pubmed/27682081
http://dx.doi.org/10.3390/microorganisms3010094
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author McTaggart, Tami L.
Beck, David A. C.
Setboonsarng, Usanisa
Shapiro, Nicole
Woyke, Tanja
Lidstrom, Mary E.
Kalyuzhnaya, Marina G.
Chistoserdova, Ludmila
author_facet McTaggart, Tami L.
Beck, David A. C.
Setboonsarng, Usanisa
Shapiro, Nicole
Woyke, Tanja
Lidstrom, Mary E.
Kalyuzhnaya, Marina G.
Chistoserdova, Ludmila
author_sort McTaggart, Tami L.
collection PubMed
description Gram-positive methylotrophic bacteria have been known for a long period of time, some serving as model organisms for characterizing the specific details of methylotrophy pathways/enzymes within this group. However, genome-based knowledge of methylotrophy within this group has been so far limited to a single species, Bacillus methanolicus (Firmicutes). The paucity of whole-genome data for Gram-positive methylotrophs limits our global understanding of methylotrophy within this group, including their roles in specific biogeochemical cycles, as well as their biotechnological potential. Here, we describe the isolation of seven novel strains of Gram-positive methylotrophs that include two strains of Bacillus and five representatives of Actinobacteria classified within two genera, Arthrobacter and Mycobacterium. We report whole-genome sequences for these isolates and present comparative analysis of the methylotrophy functional modules within these genomes. The genomic sequences of these seven novel organisms, all capable of growth on methylated amines, present an important reference dataset for understanding the genomic basis of methylotrophy in Gram-positive methylotrophic bacteria. This study is a major contribution to the field of methylotrophy, aimed at closing the gap in the genomic knowledge of methylotrophy within this diverse group of bacteria.
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spelling pubmed-50232302016-09-28 Genomics of Methylotrophy in Gram-Positive Methylamine-Utilizing Bacteria McTaggart, Tami L. Beck, David A. C. Setboonsarng, Usanisa Shapiro, Nicole Woyke, Tanja Lidstrom, Mary E. Kalyuzhnaya, Marina G. Chistoserdova, Ludmila Microorganisms Article Gram-positive methylotrophic bacteria have been known for a long period of time, some serving as model organisms for characterizing the specific details of methylotrophy pathways/enzymes within this group. However, genome-based knowledge of methylotrophy within this group has been so far limited to a single species, Bacillus methanolicus (Firmicutes). The paucity of whole-genome data for Gram-positive methylotrophs limits our global understanding of methylotrophy within this group, including their roles in specific biogeochemical cycles, as well as their biotechnological potential. Here, we describe the isolation of seven novel strains of Gram-positive methylotrophs that include two strains of Bacillus and five representatives of Actinobacteria classified within two genera, Arthrobacter and Mycobacterium. We report whole-genome sequences for these isolates and present comparative analysis of the methylotrophy functional modules within these genomes. The genomic sequences of these seven novel organisms, all capable of growth on methylated amines, present an important reference dataset for understanding the genomic basis of methylotrophy in Gram-positive methylotrophic bacteria. This study is a major contribution to the field of methylotrophy, aimed at closing the gap in the genomic knowledge of methylotrophy within this diverse group of bacteria. MDPI 2015-03-20 /pmc/articles/PMC5023230/ /pubmed/27682081 http://dx.doi.org/10.3390/microorganisms3010094 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
McTaggart, Tami L.
Beck, David A. C.
Setboonsarng, Usanisa
Shapiro, Nicole
Woyke, Tanja
Lidstrom, Mary E.
Kalyuzhnaya, Marina G.
Chistoserdova, Ludmila
Genomics of Methylotrophy in Gram-Positive Methylamine-Utilizing Bacteria
title Genomics of Methylotrophy in Gram-Positive Methylamine-Utilizing Bacteria
title_full Genomics of Methylotrophy in Gram-Positive Methylamine-Utilizing Bacteria
title_fullStr Genomics of Methylotrophy in Gram-Positive Methylamine-Utilizing Bacteria
title_full_unstemmed Genomics of Methylotrophy in Gram-Positive Methylamine-Utilizing Bacteria
title_short Genomics of Methylotrophy in Gram-Positive Methylamine-Utilizing Bacteria
title_sort genomics of methylotrophy in gram-positive methylamine-utilizing bacteria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5023230/
https://www.ncbi.nlm.nih.gov/pubmed/27682081
http://dx.doi.org/10.3390/microorganisms3010094
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