Cargando…
Methane Oxidation and Molecular Characterization of Methanotrophs from a Former Mercury Mine Impoundment
The Herman Pit, once a mercury mine, is an impoundment located in an active geothermal area. Its acidic waters are permeated by hundreds of gas seeps. One seep was sampled and found to be composed of mostly CO(2) with some CH(4) present. The δ(13)CH(4) value suggested a complex origin for the methan...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5023233/ https://www.ncbi.nlm.nih.gov/pubmed/27682090 http://dx.doi.org/10.3390/microorganisms3020290 |
_version_ | 1782453639105740800 |
---|---|
author | Baesman, Shaun M. Miller, Laurence G. Wei, Jeremy H. Cho, Yirang Matys, Emily D. Summons, Roger E. Welander, Paula V. Oremland, Ronald S. |
author_facet | Baesman, Shaun M. Miller, Laurence G. Wei, Jeremy H. Cho, Yirang Matys, Emily D. Summons, Roger E. Welander, Paula V. Oremland, Ronald S. |
author_sort | Baesman, Shaun M. |
collection | PubMed |
description | The Herman Pit, once a mercury mine, is an impoundment located in an active geothermal area. Its acidic waters are permeated by hundreds of gas seeps. One seep was sampled and found to be composed of mostly CO(2) with some CH(4) present. The δ(13)CH(4) value suggested a complex origin for the methane: i.e., a thermogenic component plus a biological methanogenic portion. The relatively (12)C-enriched CO(2) suggested a reworking of the ebullitive methane by methanotrophic bacteria. Therefore, we tested bottom sediments for their ability to consume methane by conducting aerobic incubations of slurried materials. Methane was removed from the headspace of live slurries, and subsequent additions of methane resulted in faster removal rates. This activity could be transferred to an artificial, acidic medium, indicating the presence of acidophilic or acid-tolerant methanotrophs, the latter reinforced by the observation of maximum activity at pH = 4.5 with incubated slurries. A successful extraction of sterol and hopanoid lipids characteristic of methanotrophs was achieved, and their abundances greatly increased with increased sediment methane consumption. DNA extracted from methane-oxidizing enrichment cultures was amplified and sequenced for pmoA genes that aligned with methanotrophic members of the Gammaproteobacteria. An enrichment culture was established that grew in an acidic (pH 4.5) medium via methane oxidation. |
format | Online Article Text |
id | pubmed-5023233 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-50232332016-09-28 Methane Oxidation and Molecular Characterization of Methanotrophs from a Former Mercury Mine Impoundment Baesman, Shaun M. Miller, Laurence G. Wei, Jeremy H. Cho, Yirang Matys, Emily D. Summons, Roger E. Welander, Paula V. Oremland, Ronald S. Microorganisms Article The Herman Pit, once a mercury mine, is an impoundment located in an active geothermal area. Its acidic waters are permeated by hundreds of gas seeps. One seep was sampled and found to be composed of mostly CO(2) with some CH(4) present. The δ(13)CH(4) value suggested a complex origin for the methane: i.e., a thermogenic component plus a biological methanogenic portion. The relatively (12)C-enriched CO(2) suggested a reworking of the ebullitive methane by methanotrophic bacteria. Therefore, we tested bottom sediments for their ability to consume methane by conducting aerobic incubations of slurried materials. Methane was removed from the headspace of live slurries, and subsequent additions of methane resulted in faster removal rates. This activity could be transferred to an artificial, acidic medium, indicating the presence of acidophilic or acid-tolerant methanotrophs, the latter reinforced by the observation of maximum activity at pH = 4.5 with incubated slurries. A successful extraction of sterol and hopanoid lipids characteristic of methanotrophs was achieved, and their abundances greatly increased with increased sediment methane consumption. DNA extracted from methane-oxidizing enrichment cultures was amplified and sequenced for pmoA genes that aligned with methanotrophic members of the Gammaproteobacteria. An enrichment culture was established that grew in an acidic (pH 4.5) medium via methane oxidation. MDPI 2015-06-23 /pmc/articles/PMC5023233/ /pubmed/27682090 http://dx.doi.org/10.3390/microorganisms3020290 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Baesman, Shaun M. Miller, Laurence G. Wei, Jeremy H. Cho, Yirang Matys, Emily D. Summons, Roger E. Welander, Paula V. Oremland, Ronald S. Methane Oxidation and Molecular Characterization of Methanotrophs from a Former Mercury Mine Impoundment |
title | Methane Oxidation and Molecular Characterization of Methanotrophs from a Former Mercury Mine Impoundment |
title_full | Methane Oxidation and Molecular Characterization of Methanotrophs from a Former Mercury Mine Impoundment |
title_fullStr | Methane Oxidation and Molecular Characterization of Methanotrophs from a Former Mercury Mine Impoundment |
title_full_unstemmed | Methane Oxidation and Molecular Characterization of Methanotrophs from a Former Mercury Mine Impoundment |
title_short | Methane Oxidation and Molecular Characterization of Methanotrophs from a Former Mercury Mine Impoundment |
title_sort | methane oxidation and molecular characterization of methanotrophs from a former mercury mine impoundment |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5023233/ https://www.ncbi.nlm.nih.gov/pubmed/27682090 http://dx.doi.org/10.3390/microorganisms3020290 |
work_keys_str_mv | AT baesmanshaunm methaneoxidationandmolecularcharacterizationofmethanotrophsfromaformermercurymineimpoundment AT millerlaurenceg methaneoxidationandmolecularcharacterizationofmethanotrophsfromaformermercurymineimpoundment AT weijeremyh methaneoxidationandmolecularcharacterizationofmethanotrophsfromaformermercurymineimpoundment AT choyirang methaneoxidationandmolecularcharacterizationofmethanotrophsfromaformermercurymineimpoundment AT matysemilyd methaneoxidationandmolecularcharacterizationofmethanotrophsfromaformermercurymineimpoundment AT summonsrogere methaneoxidationandmolecularcharacterizationofmethanotrophsfromaformermercurymineimpoundment AT welanderpaulav methaneoxidationandmolecularcharacterizationofmethanotrophsfromaformermercurymineimpoundment AT oremlandronalds methaneoxidationandmolecularcharacterizationofmethanotrophsfromaformermercurymineimpoundment |