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Generation of PHB from Spent Sulfite Liquor Using Halophilic Microorganisms

Halophilic microorganisms thrive at elevated concentrations of sodium chloride up to saturation and are capable of growing on a wide variety of carbon sources like various organic acids, hexose and also pentose sugars. Hence, the biotechnological application of these microorganisms can cover many as...

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Autores principales: Weissgram, Michaela, Gstöttner, Janina, Lorantfy, Bettina, Tenhaken, Raimund, Herwig, Christoph, Weber, Hedda K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5023234/
https://www.ncbi.nlm.nih.gov/pubmed/27682089
http://dx.doi.org/10.3390/microorganisms3020268
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author Weissgram, Michaela
Gstöttner, Janina
Lorantfy, Bettina
Tenhaken, Raimund
Herwig, Christoph
Weber, Hedda K.
author_facet Weissgram, Michaela
Gstöttner, Janina
Lorantfy, Bettina
Tenhaken, Raimund
Herwig, Christoph
Weber, Hedda K.
author_sort Weissgram, Michaela
collection PubMed
description Halophilic microorganisms thrive at elevated concentrations of sodium chloride up to saturation and are capable of growing on a wide variety of carbon sources like various organic acids, hexose and also pentose sugars. Hence, the biotechnological application of these microorganisms can cover many aspects, such as the treatment of hypersaline waste streams of different origin. Due to the fact that the high osmotic pressure of hypersaline environments reduces the risk of contamination, the capacity for cost-effective non-sterile cultivation can make extreme halophilic microorganisms potentially valuable organisms for biotechnological applications. In this contribution, the stepwise use of screening approaches, employing design of experiment (DoE) on model media and subsequently using industrial waste as substrate have been implemented to investigate the applicability of halophiles to generate PHB from the industrial waste stream spent sulfite liquor (SSL). The production of PHB on model media as well as dilutions of industrial substrate in a complex medium has been screened for by fluorescence microscopy using Nile Blue staining. Screening was used to investigate the ability of halophilic microorganisms to withstand the inhibiting substances of the waste stream without negatively affecting PHB production. It could be shown that neither single inhibiting substances nor a mixture thereof inhibited growth in the investigated range, hence, leaving the question on the inhibiting mechanisms open. However, it could be demonstrated that some haloarchaea and halophilic bacteria are able to produce PHB when cultivated on 3.3% w/w dry matter spent sulfite liquor, whereas H. halophila was even able to thrive on 6.6% w/w dry matter spent sulfite liquor and still produce PHB.
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spelling pubmed-50232342016-09-28 Generation of PHB from Spent Sulfite Liquor Using Halophilic Microorganisms Weissgram, Michaela Gstöttner, Janina Lorantfy, Bettina Tenhaken, Raimund Herwig, Christoph Weber, Hedda K. Microorganisms Article Halophilic microorganisms thrive at elevated concentrations of sodium chloride up to saturation and are capable of growing on a wide variety of carbon sources like various organic acids, hexose and also pentose sugars. Hence, the biotechnological application of these microorganisms can cover many aspects, such as the treatment of hypersaline waste streams of different origin. Due to the fact that the high osmotic pressure of hypersaline environments reduces the risk of contamination, the capacity for cost-effective non-sterile cultivation can make extreme halophilic microorganisms potentially valuable organisms for biotechnological applications. In this contribution, the stepwise use of screening approaches, employing design of experiment (DoE) on model media and subsequently using industrial waste as substrate have been implemented to investigate the applicability of halophiles to generate PHB from the industrial waste stream spent sulfite liquor (SSL). The production of PHB on model media as well as dilutions of industrial substrate in a complex medium has been screened for by fluorescence microscopy using Nile Blue staining. Screening was used to investigate the ability of halophilic microorganisms to withstand the inhibiting substances of the waste stream without negatively affecting PHB production. It could be shown that neither single inhibiting substances nor a mixture thereof inhibited growth in the investigated range, hence, leaving the question on the inhibiting mechanisms open. However, it could be demonstrated that some haloarchaea and halophilic bacteria are able to produce PHB when cultivated on 3.3% w/w dry matter spent sulfite liquor, whereas H. halophila was even able to thrive on 6.6% w/w dry matter spent sulfite liquor and still produce PHB. MDPI 2015-06-08 /pmc/articles/PMC5023234/ /pubmed/27682089 http://dx.doi.org/10.3390/microorganisms3020268 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Weissgram, Michaela
Gstöttner, Janina
Lorantfy, Bettina
Tenhaken, Raimund
Herwig, Christoph
Weber, Hedda K.
Generation of PHB from Spent Sulfite Liquor Using Halophilic Microorganisms
title Generation of PHB from Spent Sulfite Liquor Using Halophilic Microorganisms
title_full Generation of PHB from Spent Sulfite Liquor Using Halophilic Microorganisms
title_fullStr Generation of PHB from Spent Sulfite Liquor Using Halophilic Microorganisms
title_full_unstemmed Generation of PHB from Spent Sulfite Liquor Using Halophilic Microorganisms
title_short Generation of PHB from Spent Sulfite Liquor Using Halophilic Microorganisms
title_sort generation of phb from spent sulfite liquor using halophilic microorganisms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5023234/
https://www.ncbi.nlm.nih.gov/pubmed/27682089
http://dx.doi.org/10.3390/microorganisms3020268
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