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Temporal Study of the Microbial Diversity of the North Arm of Great Salt Lake, Utah, U.S.

We employed a temporal sampling approach to understand how the microbial diversity may shift in the north arm of Great Salt Lake, Utah, U.S. To determine how variations in seasonal environmental factors affect microbial communities, length heterogeneity PCR fingerprinting was performed using consens...

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Detalles Bibliográficos
Autores principales: Almeida-Dalmet, Swati, Sikaroodi, Masoumeh, Gillevet, Patrick M., Litchfield, Carol D., Baxter, Bonnie K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5023243/
https://www.ncbi.nlm.nih.gov/pubmed/27682091
http://dx.doi.org/10.3390/microorganisms3030310
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author Almeida-Dalmet, Swati
Sikaroodi, Masoumeh
Gillevet, Patrick M.
Litchfield, Carol D.
Baxter, Bonnie K.
author_facet Almeida-Dalmet, Swati
Sikaroodi, Masoumeh
Gillevet, Patrick M.
Litchfield, Carol D.
Baxter, Bonnie K.
author_sort Almeida-Dalmet, Swati
collection PubMed
description We employed a temporal sampling approach to understand how the microbial diversity may shift in the north arm of Great Salt Lake, Utah, U.S. To determine how variations in seasonal environmental factors affect microbial communities, length heterogeneity PCR fingerprinting was performed using consensus primers for the domain Bacteria, and the haloarchaea. The archaeal fingerprints showed similarities during 2003 and 2004, but this diversity changed during the remaining two years of the study, 2005 and 2006. We also performed molecular phylogenetic analysis of the 16S rRNA genes of the whole microbial community to characterize the taxa in the samples. Our results indicated that in the domain, Bacteria, the Salinibacter group dominated the populations in all samplings. However, in the case of Archaea, as noted by LIBSHUFF for phylogenetic relatedness analysis, many of the temporal communities were distinct from each other, and changes in community composition did not track with environmental parameters. Around 20–23 different phylotypes, as revealed by rarefaction, predominated at different periods of the year. Some phylotypes, such as Haloquadradum, were present year-round although they changed in their abundance in different samplings, which may indicate that these species are affected by biotic factors, such as nutrients or viruses, that are independent of seasonal temperature dynamics.
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spelling pubmed-50232432016-09-28 Temporal Study of the Microbial Diversity of the North Arm of Great Salt Lake, Utah, U.S. Almeida-Dalmet, Swati Sikaroodi, Masoumeh Gillevet, Patrick M. Litchfield, Carol D. Baxter, Bonnie K. Microorganisms Article We employed a temporal sampling approach to understand how the microbial diversity may shift in the north arm of Great Salt Lake, Utah, U.S. To determine how variations in seasonal environmental factors affect microbial communities, length heterogeneity PCR fingerprinting was performed using consensus primers for the domain Bacteria, and the haloarchaea. The archaeal fingerprints showed similarities during 2003 and 2004, but this diversity changed during the remaining two years of the study, 2005 and 2006. We also performed molecular phylogenetic analysis of the 16S rRNA genes of the whole microbial community to characterize the taxa in the samples. Our results indicated that in the domain, Bacteria, the Salinibacter group dominated the populations in all samplings. However, in the case of Archaea, as noted by LIBSHUFF for phylogenetic relatedness analysis, many of the temporal communities were distinct from each other, and changes in community composition did not track with environmental parameters. Around 20–23 different phylotypes, as revealed by rarefaction, predominated at different periods of the year. Some phylotypes, such as Haloquadradum, were present year-round although they changed in their abundance in different samplings, which may indicate that these species are affected by biotic factors, such as nutrients or viruses, that are independent of seasonal temperature dynamics. MDPI 2015-07-02 /pmc/articles/PMC5023243/ /pubmed/27682091 http://dx.doi.org/10.3390/microorganisms3030310 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Almeida-Dalmet, Swati
Sikaroodi, Masoumeh
Gillevet, Patrick M.
Litchfield, Carol D.
Baxter, Bonnie K.
Temporal Study of the Microbial Diversity of the North Arm of Great Salt Lake, Utah, U.S.
title Temporal Study of the Microbial Diversity of the North Arm of Great Salt Lake, Utah, U.S.
title_full Temporal Study of the Microbial Diversity of the North Arm of Great Salt Lake, Utah, U.S.
title_fullStr Temporal Study of the Microbial Diversity of the North Arm of Great Salt Lake, Utah, U.S.
title_full_unstemmed Temporal Study of the Microbial Diversity of the North Arm of Great Salt Lake, Utah, U.S.
title_short Temporal Study of the Microbial Diversity of the North Arm of Great Salt Lake, Utah, U.S.
title_sort temporal study of the microbial diversity of the north arm of great salt lake, utah, u.s.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5023243/
https://www.ncbi.nlm.nih.gov/pubmed/27682091
http://dx.doi.org/10.3390/microorganisms3030310
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