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Novel Methanotrophs of the Family Methylococcaceae from Different Geographical Regions and Habitats
Terrestrial methane seeps and rice paddy fields are important ecosystems in the methane cycle. Methanotrophic bacteria in these ecosystems play a key role in reducing methane emission into the atmosphere. Here, we describe three novel methanotrophs, designated BRS-K6, GFS-K6 and AK-K6, which were re...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5023254/ https://www.ncbi.nlm.nih.gov/pubmed/27682101 http://dx.doi.org/10.3390/microorganisms3030484 |
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author | Islam, Tajul Larsen, Øivind Torsvik, Vigdis Øvreås, Lise Panosyan, Hovik Murrell, J. Colin Birkeland, Nils-Kåre Bodrossy, Levente |
author_facet | Islam, Tajul Larsen, Øivind Torsvik, Vigdis Øvreås, Lise Panosyan, Hovik Murrell, J. Colin Birkeland, Nils-Kåre Bodrossy, Levente |
author_sort | Islam, Tajul |
collection | PubMed |
description | Terrestrial methane seeps and rice paddy fields are important ecosystems in the methane cycle. Methanotrophic bacteria in these ecosystems play a key role in reducing methane emission into the atmosphere. Here, we describe three novel methanotrophs, designated BRS-K6, GFS-K6 and AK-K6, which were recovered from three different habitats in contrasting geographic regions and ecosystems: waterlogged rice-field soil and methane seep pond sediments from Bangladesh; and warm spring sediments from Armenia. All isolates had a temperature range for growth of 8–35 °C (optimal 25–28 °C) and a pH range of 5.0–7.5 (optimal 6.4–7.0). 16S rRNA gene sequences showed that they were new gammaproteobacterial methanotrophs, which form a separate clade in the family Methylococcaceae. They fell into a cluster with thermotolerant and mesophilic growth tendency, comprising the genera Methylocaldum-Methylococcus-Methyloparacoccus-Methylogaea. So far, growth below 15 °C of methanotrophs from this cluster has not been reported. The strains possessed type I intracytoplasmic membranes. The genes pmoA, mxaF, cbbL, nifH were detected, but no mmoX gene was found. Each strain probably represents a novel species either belonging to the same novel genus or each may even represent separate genera. These isolates extend our knowledge of methanotrophic Gammaproteobacteria and their physiology and adaptation to different ecosystems. |
format | Online Article Text |
id | pubmed-5023254 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-50232542016-09-28 Novel Methanotrophs of the Family Methylococcaceae from Different Geographical Regions and Habitats Islam, Tajul Larsen, Øivind Torsvik, Vigdis Øvreås, Lise Panosyan, Hovik Murrell, J. Colin Birkeland, Nils-Kåre Bodrossy, Levente Microorganisms Article Terrestrial methane seeps and rice paddy fields are important ecosystems in the methane cycle. Methanotrophic bacteria in these ecosystems play a key role in reducing methane emission into the atmosphere. Here, we describe three novel methanotrophs, designated BRS-K6, GFS-K6 and AK-K6, which were recovered from three different habitats in contrasting geographic regions and ecosystems: waterlogged rice-field soil and methane seep pond sediments from Bangladesh; and warm spring sediments from Armenia. All isolates had a temperature range for growth of 8–35 °C (optimal 25–28 °C) and a pH range of 5.0–7.5 (optimal 6.4–7.0). 16S rRNA gene sequences showed that they were new gammaproteobacterial methanotrophs, which form a separate clade in the family Methylococcaceae. They fell into a cluster with thermotolerant and mesophilic growth tendency, comprising the genera Methylocaldum-Methylococcus-Methyloparacoccus-Methylogaea. So far, growth below 15 °C of methanotrophs from this cluster has not been reported. The strains possessed type I intracytoplasmic membranes. The genes pmoA, mxaF, cbbL, nifH were detected, but no mmoX gene was found. Each strain probably represents a novel species either belonging to the same novel genus or each may even represent separate genera. These isolates extend our knowledge of methanotrophic Gammaproteobacteria and their physiology and adaptation to different ecosystems. MDPI 2015-08-21 /pmc/articles/PMC5023254/ /pubmed/27682101 http://dx.doi.org/10.3390/microorganisms3030484 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Islam, Tajul Larsen, Øivind Torsvik, Vigdis Øvreås, Lise Panosyan, Hovik Murrell, J. Colin Birkeland, Nils-Kåre Bodrossy, Levente Novel Methanotrophs of the Family Methylococcaceae from Different Geographical Regions and Habitats |
title | Novel Methanotrophs of the Family Methylococcaceae from Different Geographical Regions and Habitats |
title_full | Novel Methanotrophs of the Family Methylococcaceae from Different Geographical Regions and Habitats |
title_fullStr | Novel Methanotrophs of the Family Methylococcaceae from Different Geographical Regions and Habitats |
title_full_unstemmed | Novel Methanotrophs of the Family Methylococcaceae from Different Geographical Regions and Habitats |
title_short | Novel Methanotrophs of the Family Methylococcaceae from Different Geographical Regions and Habitats |
title_sort | novel methanotrophs of the family methylococcaceae from different geographical regions and habitats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5023254/ https://www.ncbi.nlm.nih.gov/pubmed/27682101 http://dx.doi.org/10.3390/microorganisms3030484 |
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