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Promoting differentiation of cultured myoblasts using biomimetic surfaces that present alpha-laminin-2 peptides
Traditionally, muscle cell lines are cultured on glass coverslips and differentiated to investigate myoblast fusion and differentiation. Efficient differentiation of myoblasts produces a dense network of myotubes with the correct organisation for contraction. Here we have tested the ability of artif...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5023573/ https://www.ncbi.nlm.nih.gov/pubmed/27507643 http://dx.doi.org/10.1007/s10616-016-0006-y |
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author | Parker, Francine White, Kathryn Phillips, Siȏn Peckham, Michelle |
author_facet | Parker, Francine White, Kathryn Phillips, Siȏn Peckham, Michelle |
author_sort | Parker, Francine |
collection | PubMed |
description | Traditionally, muscle cell lines are cultured on glass coverslips and differentiated to investigate myoblast fusion and differentiation. Efficient differentiation of myoblasts produces a dense network of myotubes with the correct organisation for contraction. Here we have tested the ability of artificially generated, precisely controlled peptide surfaces to enhance the efficiency of myoblast differentiation. We focused on specific short peptides from α-laminin-2 (IKVSV, VQLRNGFPYFSY and GLLFYMARINHA) as well as residues 15–155 from FGF1. We tested if these peptides in isolation, and/or in combination promoted muscle differentiation in culture, by promoting fusion and/or by improving sarcomere organisation. The majority of these peptides promoted fusion and differentiation in two different mouse myogenic cell lines and in primary human myoblasts. The additive effects of all four peptides gave the best results for both mouse cell lines tested, while primary human cell cultures differentiated equally well on most peptide surfaces tested. These data show that a mixture of short biomimetic peptides can reliably promote differentiation in mouse and human myoblasts. |
format | Online Article Text |
id | pubmed-5023573 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-50235732016-09-27 Promoting differentiation of cultured myoblasts using biomimetic surfaces that present alpha-laminin-2 peptides Parker, Francine White, Kathryn Phillips, Siȏn Peckham, Michelle Cytotechnology Short Communication Traditionally, muscle cell lines are cultured on glass coverslips and differentiated to investigate myoblast fusion and differentiation. Efficient differentiation of myoblasts produces a dense network of myotubes with the correct organisation for contraction. Here we have tested the ability of artificially generated, precisely controlled peptide surfaces to enhance the efficiency of myoblast differentiation. We focused on specific short peptides from α-laminin-2 (IKVSV, VQLRNGFPYFSY and GLLFYMARINHA) as well as residues 15–155 from FGF1. We tested if these peptides in isolation, and/or in combination promoted muscle differentiation in culture, by promoting fusion and/or by improving sarcomere organisation. The majority of these peptides promoted fusion and differentiation in two different mouse myogenic cell lines and in primary human myoblasts. The additive effects of all four peptides gave the best results for both mouse cell lines tested, while primary human cell cultures differentiated equally well on most peptide surfaces tested. These data show that a mixture of short biomimetic peptides can reliably promote differentiation in mouse and human myoblasts. Springer Netherlands 2016-08-09 2016-10 /pmc/articles/PMC5023573/ /pubmed/27507643 http://dx.doi.org/10.1007/s10616-016-0006-y Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Short Communication Parker, Francine White, Kathryn Phillips, Siȏn Peckham, Michelle Promoting differentiation of cultured myoblasts using biomimetic surfaces that present alpha-laminin-2 peptides |
title | Promoting differentiation of cultured myoblasts using biomimetic surfaces that present alpha-laminin-2 peptides |
title_full | Promoting differentiation of cultured myoblasts using biomimetic surfaces that present alpha-laminin-2 peptides |
title_fullStr | Promoting differentiation of cultured myoblasts using biomimetic surfaces that present alpha-laminin-2 peptides |
title_full_unstemmed | Promoting differentiation of cultured myoblasts using biomimetic surfaces that present alpha-laminin-2 peptides |
title_short | Promoting differentiation of cultured myoblasts using biomimetic surfaces that present alpha-laminin-2 peptides |
title_sort | promoting differentiation of cultured myoblasts using biomimetic surfaces that present alpha-laminin-2 peptides |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5023573/ https://www.ncbi.nlm.nih.gov/pubmed/27507643 http://dx.doi.org/10.1007/s10616-016-0006-y |
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