Conjugated Polymer Nanoparticles for Label‐Free and Bioconjugate‐Recognized DNA Sensing in Serum

Hybridbio/‐synthetic sensory conjugated polymer nanoparticles (CPNs) are developed for selective label‐free detection of target ssDNA in serum. Carboxylic acid‐functionalized anionic polyfluorene nanoparticles are rationally designed as signal amplifying unit to bioconjugate with amine functionalize...

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Detalles Bibliográficos
Autores principales: Bao, Biqing, Ma, Mingfeng, Zai, Huafeng, Zhang, Lei, Fu, Nina, Huang, Wei, Wang, Lianhui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2015
Materias:
Full Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5024028/
https://www.ncbi.nlm.nih.gov/pubmed/27668149
http://dx.doi.org/10.1002/advs.201400009
Descripción
Sumario:Hybridbio/‐synthetic sensory conjugated polymer nanoparticles (CPNs) are developed for selective label‐free detection of target ssDNA in serum. Carboxylic acid‐functionalized anionic polyfluorene nanoparticles are rationally designed as signal amplifying unit to bioconjugate with amine functionalized single stranded oligonucleotides as a receptor. The covalent DNA coating can significantly improve the photostability of the DNA‐bioconjugated CPNs over a wide range of buffer conditions. Better ssDNA discrimination for the DNA‐bioconjugated CPNs sensor is achieved owing to increased interchain interactions and more efficient exciton transport in nanoparticles. The distinguishable fluorescent color for DNA‐bioconjugated CPNs in the presence of target ssDNA allows naked‐eye detection of ssDNA under UV irradiation.

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