Cargando…
Unique roles of the unfolded protein response pathway in fungal development and differentiation
Cryptococcus neoformans, a global fungal meningitis pathogen, employs the unfolded protein response pathway. This pathway, which consists of an evolutionarily conserved Ire1 kinase/endoribonuclease and a unique transcription factor (Hxl1), modulates the endoplasmic reticulum stress response and path...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5024300/ https://www.ncbi.nlm.nih.gov/pubmed/27629591 http://dx.doi.org/10.1038/srep33413 |
_version_ | 1782453783407624192 |
---|---|
author | Jung, Kwang-Woo So, Yee-Seul Bahn, Yong-Sun |
author_facet | Jung, Kwang-Woo So, Yee-Seul Bahn, Yong-Sun |
author_sort | Jung, Kwang-Woo |
collection | PubMed |
description | Cryptococcus neoformans, a global fungal meningitis pathogen, employs the unfolded protein response pathway. This pathway, which consists of an evolutionarily conserved Ire1 kinase/endoribonuclease and a unique transcription factor (Hxl1), modulates the endoplasmic reticulum stress response and pathogenicity. Here, we report that the unfolded protein response pathway governs sexual and unisexual differentiation of C. neoformans in an Ire1-dependent but Hxl1-independent manner. The ire1∆ mutants showed defects in sexual mating, with reduced cell fusion and pheromone-mediated formation of the conjugation tube. Unexpectedly, these mating defects did not result from defective pheromone production because expression of the mating pheromone gene (MFα1) was strongly induced in the ire1∆ mutant. Ire1 controls sexual differentiation by modulating the function of the molecular chaperone Kar2 and by regulating mating-induced localisation of mating pheromone transporter (Ste6) and receptor (Ste3/Cprα). Deletion of IRE1, but not HXL1, also caused significant defects in unisexual differentiation in a Kar2-independent manner. Moreover, we showed that Rim101 is a novel downstream factor of Ire1 for production of the capsule, which is a unique structural determinant of C. neoformans virulence. Therefore, Ire1 uniquely regulates fungal development and differentiation in an Hxl1-independent manner. |
format | Online Article Text |
id | pubmed-5024300 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50243002016-09-20 Unique roles of the unfolded protein response pathway in fungal development and differentiation Jung, Kwang-Woo So, Yee-Seul Bahn, Yong-Sun Sci Rep Article Cryptococcus neoformans, a global fungal meningitis pathogen, employs the unfolded protein response pathway. This pathway, which consists of an evolutionarily conserved Ire1 kinase/endoribonuclease and a unique transcription factor (Hxl1), modulates the endoplasmic reticulum stress response and pathogenicity. Here, we report that the unfolded protein response pathway governs sexual and unisexual differentiation of C. neoformans in an Ire1-dependent but Hxl1-independent manner. The ire1∆ mutants showed defects in sexual mating, with reduced cell fusion and pheromone-mediated formation of the conjugation tube. Unexpectedly, these mating defects did not result from defective pheromone production because expression of the mating pheromone gene (MFα1) was strongly induced in the ire1∆ mutant. Ire1 controls sexual differentiation by modulating the function of the molecular chaperone Kar2 and by regulating mating-induced localisation of mating pheromone transporter (Ste6) and receptor (Ste3/Cprα). Deletion of IRE1, but not HXL1, also caused significant defects in unisexual differentiation in a Kar2-independent manner. Moreover, we showed that Rim101 is a novel downstream factor of Ire1 for production of the capsule, which is a unique structural determinant of C. neoformans virulence. Therefore, Ire1 uniquely regulates fungal development and differentiation in an Hxl1-independent manner. Nature Publishing Group 2016-09-15 /pmc/articles/PMC5024300/ /pubmed/27629591 http://dx.doi.org/10.1038/srep33413 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Jung, Kwang-Woo So, Yee-Seul Bahn, Yong-Sun Unique roles of the unfolded protein response pathway in fungal development and differentiation |
title | Unique roles of the unfolded protein response pathway in fungal development and differentiation |
title_full | Unique roles of the unfolded protein response pathway in fungal development and differentiation |
title_fullStr | Unique roles of the unfolded protein response pathway in fungal development and differentiation |
title_full_unstemmed | Unique roles of the unfolded protein response pathway in fungal development and differentiation |
title_short | Unique roles of the unfolded protein response pathway in fungal development and differentiation |
title_sort | unique roles of the unfolded protein response pathway in fungal development and differentiation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5024300/ https://www.ncbi.nlm.nih.gov/pubmed/27629591 http://dx.doi.org/10.1038/srep33413 |
work_keys_str_mv | AT jungkwangwoo uniquerolesoftheunfoldedproteinresponsepathwayinfungaldevelopmentanddifferentiation AT soyeeseul uniquerolesoftheunfoldedproteinresponsepathwayinfungaldevelopmentanddifferentiation AT bahnyongsun uniquerolesoftheunfoldedproteinresponsepathwayinfungaldevelopmentanddifferentiation |