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Adapting extracellular matrix proteomics for clinical studies on cardiac remodeling post-myocardial infarction

Following myocardial infarction (MI), the left ventricle (LV) undergoes a series of cardiac wound healing responses that involve stimulation of robust inflammation to clear necrotic myocytes and tissue debris and induction of extracellular matrix (ECM) protein synthesis to generate a scar. Proteomic...

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Autores principales: Lindsey, Merry L., Hall, Michael E., Harmancey, Romain, Ma, Yonggang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5024439/
https://www.ncbi.nlm.nih.gov/pubmed/27651752
http://dx.doi.org/10.1186/s12014-016-9120-2
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author Lindsey, Merry L.
Hall, Michael E.
Harmancey, Romain
Ma, Yonggang
author_facet Lindsey, Merry L.
Hall, Michael E.
Harmancey, Romain
Ma, Yonggang
author_sort Lindsey, Merry L.
collection PubMed
description Following myocardial infarction (MI), the left ventricle (LV) undergoes a series of cardiac wound healing responses that involve stimulation of robust inflammation to clear necrotic myocytes and tissue debris and induction of extracellular matrix (ECM) protein synthesis to generate a scar. Proteomic strategies provide us with a means to index the ECM proteins expressed in the LV, quantify amounts, determine functions, and explore interactions. This review will focus on the efforts taken in the proteomics research field that have expanded our understanding of post-MI LV remodeling, concentrating on the strengths and limitations of different proteomic approaches to glean information that is specific to ECM turnover in the post-MI setting. We will discuss how recent advances in sample preparation and labeling protocols increase our successes at detecting components of the cardiac ECM proteome. We will summarize how proteomic approaches, focusing on the ECM compartment, have progressed over time to current gel-free methods using decellularized fractions or labeling strategies that will be useful for clinical applications. This review will provide an overview of how cardiac ECM proteomics has evolved over the last decade and will provide insight into future directions that will drive forward our understanding of cardiac ECM turnover in the post-MI LV.
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spelling pubmed-50244392016-09-20 Adapting extracellular matrix proteomics for clinical studies on cardiac remodeling post-myocardial infarction Lindsey, Merry L. Hall, Michael E. Harmancey, Romain Ma, Yonggang Clin Proteomics Review Following myocardial infarction (MI), the left ventricle (LV) undergoes a series of cardiac wound healing responses that involve stimulation of robust inflammation to clear necrotic myocytes and tissue debris and induction of extracellular matrix (ECM) protein synthesis to generate a scar. Proteomic strategies provide us with a means to index the ECM proteins expressed in the LV, quantify amounts, determine functions, and explore interactions. This review will focus on the efforts taken in the proteomics research field that have expanded our understanding of post-MI LV remodeling, concentrating on the strengths and limitations of different proteomic approaches to glean information that is specific to ECM turnover in the post-MI setting. We will discuss how recent advances in sample preparation and labeling protocols increase our successes at detecting components of the cardiac ECM proteome. We will summarize how proteomic approaches, focusing on the ECM compartment, have progressed over time to current gel-free methods using decellularized fractions or labeling strategies that will be useful for clinical applications. This review will provide an overview of how cardiac ECM proteomics has evolved over the last decade and will provide insight into future directions that will drive forward our understanding of cardiac ECM turnover in the post-MI LV. BioMed Central 2016-09-15 /pmc/articles/PMC5024439/ /pubmed/27651752 http://dx.doi.org/10.1186/s12014-016-9120-2 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Review
Lindsey, Merry L.
Hall, Michael E.
Harmancey, Romain
Ma, Yonggang
Adapting extracellular matrix proteomics for clinical studies on cardiac remodeling post-myocardial infarction
title Adapting extracellular matrix proteomics for clinical studies on cardiac remodeling post-myocardial infarction
title_full Adapting extracellular matrix proteomics for clinical studies on cardiac remodeling post-myocardial infarction
title_fullStr Adapting extracellular matrix proteomics for clinical studies on cardiac remodeling post-myocardial infarction
title_full_unstemmed Adapting extracellular matrix proteomics for clinical studies on cardiac remodeling post-myocardial infarction
title_short Adapting extracellular matrix proteomics for clinical studies on cardiac remodeling post-myocardial infarction
title_sort adapting extracellular matrix proteomics for clinical studies on cardiac remodeling post-myocardial infarction
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5024439/
https://www.ncbi.nlm.nih.gov/pubmed/27651752
http://dx.doi.org/10.1186/s12014-016-9120-2
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