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Ex Vivo Confocal Spectroscopy of Autofluorescence in Age-Related Macular Degeneration
PURPOSE: We investigated the autofluorescence (AF) signature of the microscopic features of retina with age-related macular degeneration (AMD) using 488 nm excitation. METHODS: The globes of four donors with AMD and four age-matched controls were embedded in paraffin and sectioned through the macula...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5024989/ https://www.ncbi.nlm.nih.gov/pubmed/27631087 http://dx.doi.org/10.1371/journal.pone.0162869 |
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author | Kaluzny, Joel Purta, Patryk Poskin, Zach Rogers, Jeremy D. Fawzi, Amani A. |
author_facet | Kaluzny, Joel Purta, Patryk Poskin, Zach Rogers, Jeremy D. Fawzi, Amani A. |
author_sort | Kaluzny, Joel |
collection | PubMed |
description | PURPOSE: We investigated the autofluorescence (AF) signature of the microscopic features of retina with age-related macular degeneration (AMD) using 488 nm excitation. METHODS: The globes of four donors with AMD and four age-matched controls were embedded in paraffin and sectioned through the macula. Sections were excited using a 488 nm argon laser, and the AF emission was captured using a laser scanning confocal microscope (496–610 nm, 6 nm resolution). The data cubes were then analyzed to compare peak emission spectra between the AMD and the controls. Microscopic features, including individual lipofuscin and melanolipofuscin granules, Bruch’s Membrane, as well macroscopic features, were considered. RESULTS: Overall, the AMD eyes showed a trend of blue-shifted emission peaks compared with the controls. These differences were statistically significant when considering the emission of the combined RPE/Bruch’s Membrane across all the tissue cross-sections (p = 0.02). CONCLUSIONS: The AF signatures of ex vivo AMD RPE/BrM show blue-shifted emission spectra (488 nm excitation) compared with the control tissue. The magnitude of these differences is small (~4 nm) and highlights the potential challenges of detecting these subtle spectral differences in vivo. |
format | Online Article Text |
id | pubmed-5024989 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-50249892016-09-27 Ex Vivo Confocal Spectroscopy of Autofluorescence in Age-Related Macular Degeneration Kaluzny, Joel Purta, Patryk Poskin, Zach Rogers, Jeremy D. Fawzi, Amani A. PLoS One Research Article PURPOSE: We investigated the autofluorescence (AF) signature of the microscopic features of retina with age-related macular degeneration (AMD) using 488 nm excitation. METHODS: The globes of four donors with AMD and four age-matched controls were embedded in paraffin and sectioned through the macula. Sections were excited using a 488 nm argon laser, and the AF emission was captured using a laser scanning confocal microscope (496–610 nm, 6 nm resolution). The data cubes were then analyzed to compare peak emission spectra between the AMD and the controls. Microscopic features, including individual lipofuscin and melanolipofuscin granules, Bruch’s Membrane, as well macroscopic features, were considered. RESULTS: Overall, the AMD eyes showed a trend of blue-shifted emission peaks compared with the controls. These differences were statistically significant when considering the emission of the combined RPE/Bruch’s Membrane across all the tissue cross-sections (p = 0.02). CONCLUSIONS: The AF signatures of ex vivo AMD RPE/BrM show blue-shifted emission spectra (488 nm excitation) compared with the control tissue. The magnitude of these differences is small (~4 nm) and highlights the potential challenges of detecting these subtle spectral differences in vivo. Public Library of Science 2016-09-15 /pmc/articles/PMC5024989/ /pubmed/27631087 http://dx.doi.org/10.1371/journal.pone.0162869 Text en © 2016 Kaluzny et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Kaluzny, Joel Purta, Patryk Poskin, Zach Rogers, Jeremy D. Fawzi, Amani A. Ex Vivo Confocal Spectroscopy of Autofluorescence in Age-Related Macular Degeneration |
title | Ex Vivo Confocal Spectroscopy of Autofluorescence in Age-Related Macular Degeneration |
title_full | Ex Vivo Confocal Spectroscopy of Autofluorescence in Age-Related Macular Degeneration |
title_fullStr | Ex Vivo Confocal Spectroscopy of Autofluorescence in Age-Related Macular Degeneration |
title_full_unstemmed | Ex Vivo Confocal Spectroscopy of Autofluorescence in Age-Related Macular Degeneration |
title_short | Ex Vivo Confocal Spectroscopy of Autofluorescence in Age-Related Macular Degeneration |
title_sort | ex vivo confocal spectroscopy of autofluorescence in age-related macular degeneration |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5024989/ https://www.ncbi.nlm.nih.gov/pubmed/27631087 http://dx.doi.org/10.1371/journal.pone.0162869 |
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