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Analysis of Polymorphic Membrane Protein Expression in Cultured Cells Identifies PmpA and PmpH of Chlamydia psittaci as Candidate Factors in Pathogenesis and Immunity to Infection

The polymorphic membrane protein (Pmp) paralogous families of Chlamydia trachomatis, Chlamydia pneumoniae and Chlamydia abortus are putative targets for Chlamydia vaccine development. To determine whether this is also the case for Pmp family members of C. psittaci, we analyzed transcription levels,...

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Autores principales: Van Lent, Sarah, De Vos, Winnok H., Huot Creasy, Heather, Marques, Patricia X., Ravel, Jacques, Vanrompay, Daisy, Bavoil, Patrik, Hsia, Ru-ching
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5025070/
https://www.ncbi.nlm.nih.gov/pubmed/27631978
http://dx.doi.org/10.1371/journal.pone.0162392
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author Van Lent, Sarah
De Vos, Winnok H.
Huot Creasy, Heather
Marques, Patricia X.
Ravel, Jacques
Vanrompay, Daisy
Bavoil, Patrik
Hsia, Ru-ching
author_facet Van Lent, Sarah
De Vos, Winnok H.
Huot Creasy, Heather
Marques, Patricia X.
Ravel, Jacques
Vanrompay, Daisy
Bavoil, Patrik
Hsia, Ru-ching
author_sort Van Lent, Sarah
collection PubMed
description The polymorphic membrane protein (Pmp) paralogous families of Chlamydia trachomatis, Chlamydia pneumoniae and Chlamydia abortus are putative targets for Chlamydia vaccine development. To determine whether this is also the case for Pmp family members of C. psittaci, we analyzed transcription levels, protein production and localization of several Pmps of C. psittaci. Pmp expression profiles were characterized using quantitative real-time PCR (RT-qPCR), immunofluorescence (IF) and immuno-electron microscopy (IEM) under normal and stress conditions. We found that PmpA was highly produced in all inclusions as early as 12 hpi in all biological replicates. In addition, PmpA and PmpH appeared to be unusually accessible to antibody as determined by both immunofluorescence and immuno-electron microscopy. Our results suggest an important role for these Pmps in the pathogenesis of C. psittaci, and make them promising candidates in vaccine development.
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spelling pubmed-50250702016-09-27 Analysis of Polymorphic Membrane Protein Expression in Cultured Cells Identifies PmpA and PmpH of Chlamydia psittaci as Candidate Factors in Pathogenesis and Immunity to Infection Van Lent, Sarah De Vos, Winnok H. Huot Creasy, Heather Marques, Patricia X. Ravel, Jacques Vanrompay, Daisy Bavoil, Patrik Hsia, Ru-ching PLoS One Research Article The polymorphic membrane protein (Pmp) paralogous families of Chlamydia trachomatis, Chlamydia pneumoniae and Chlamydia abortus are putative targets for Chlamydia vaccine development. To determine whether this is also the case for Pmp family members of C. psittaci, we analyzed transcription levels, protein production and localization of several Pmps of C. psittaci. Pmp expression profiles were characterized using quantitative real-time PCR (RT-qPCR), immunofluorescence (IF) and immuno-electron microscopy (IEM) under normal and stress conditions. We found that PmpA was highly produced in all inclusions as early as 12 hpi in all biological replicates. In addition, PmpA and PmpH appeared to be unusually accessible to antibody as determined by both immunofluorescence and immuno-electron microscopy. Our results suggest an important role for these Pmps in the pathogenesis of C. psittaci, and make them promising candidates in vaccine development. Public Library of Science 2016-09-15 /pmc/articles/PMC5025070/ /pubmed/27631978 http://dx.doi.org/10.1371/journal.pone.0162392 Text en © 2016 Van Lent et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Van Lent, Sarah
De Vos, Winnok H.
Huot Creasy, Heather
Marques, Patricia X.
Ravel, Jacques
Vanrompay, Daisy
Bavoil, Patrik
Hsia, Ru-ching
Analysis of Polymorphic Membrane Protein Expression in Cultured Cells Identifies PmpA and PmpH of Chlamydia psittaci as Candidate Factors in Pathogenesis and Immunity to Infection
title Analysis of Polymorphic Membrane Protein Expression in Cultured Cells Identifies PmpA and PmpH of Chlamydia psittaci as Candidate Factors in Pathogenesis and Immunity to Infection
title_full Analysis of Polymorphic Membrane Protein Expression in Cultured Cells Identifies PmpA and PmpH of Chlamydia psittaci as Candidate Factors in Pathogenesis and Immunity to Infection
title_fullStr Analysis of Polymorphic Membrane Protein Expression in Cultured Cells Identifies PmpA and PmpH of Chlamydia psittaci as Candidate Factors in Pathogenesis and Immunity to Infection
title_full_unstemmed Analysis of Polymorphic Membrane Protein Expression in Cultured Cells Identifies PmpA and PmpH of Chlamydia psittaci as Candidate Factors in Pathogenesis and Immunity to Infection
title_short Analysis of Polymorphic Membrane Protein Expression in Cultured Cells Identifies PmpA and PmpH of Chlamydia psittaci as Candidate Factors in Pathogenesis and Immunity to Infection
title_sort analysis of polymorphic membrane protein expression in cultured cells identifies pmpa and pmph of chlamydia psittaci as candidate factors in pathogenesis and immunity to infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5025070/
https://www.ncbi.nlm.nih.gov/pubmed/27631978
http://dx.doi.org/10.1371/journal.pone.0162392
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