A Rational Engineering Strategy for Designing Protein A-Binding Camelid Single-Domain Antibodies

Staphylococcal protein A (SpA) and streptococcal protein G (SpG) affinity chromatography are the gold standards for purifying monoclonal antibodies (mAbs) in therapeutic applications. However, camelid V(H)H single-domain Abs (sdAbs or V(H)Hs) are not bound by SpG and only sporadically bound by SpA....

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Detalles Bibliográficos
Autores principales: Henry, Kevin A., Sulea, Traian, van Faassen, Henk, Hussack, Greg, Purisima, Enrico O., MacKenzie, C. Roger, Arbabi-Ghahroudi, Mehdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5025174/
https://www.ncbi.nlm.nih.gov/pubmed/27631624
http://dx.doi.org/10.1371/journal.pone.0163113

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5025174/
https://www.ncbi.nlm.nih.gov/pubmed/27631624
http://dx.doi.org/10.1371/journal.pone.0163113

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