Cargando…

Evaluation of Genotoxic Pressure along the Sava River

In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The geno...

Descripción completa

Detalles Bibliográficos
Autores principales: Kolarević, Stoimir, Aborgiba, Mustafa, Kračun-Kolarević, Margareta, Kostić, Jovana, Simonović, Predrag, Simić, Vladica, Milošković, Aleksandra, Reischer, Georg, Farnleitner, Andreas, Gačić, Zoran, Milačič, Radmila, Zuliani, Tea, Vidmar, Janja, Pergal, Marija, Piria, Marina, Paunović, Momir, Vuković-Gačić, Branka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5025182/
https://www.ncbi.nlm.nih.gov/pubmed/27631093
http://dx.doi.org/10.1371/journal.pone.0162450
_version_ 1782453915335262208
author Kolarević, Stoimir
Aborgiba, Mustafa
Kračun-Kolarević, Margareta
Kostić, Jovana
Simonović, Predrag
Simić, Vladica
Milošković, Aleksandra
Reischer, Georg
Farnleitner, Andreas
Gačić, Zoran
Milačič, Radmila
Zuliani, Tea
Vidmar, Janja
Pergal, Marija
Piria, Marina
Paunović, Momir
Vuković-Gačić, Branka
author_facet Kolarević, Stoimir
Aborgiba, Mustafa
Kračun-Kolarević, Margareta
Kostić, Jovana
Simonović, Predrag
Simić, Vladica
Milošković, Aleksandra
Reischer, Georg
Farnleitner, Andreas
Gačić, Zoran
Milačič, Radmila
Zuliani, Tea
Vidmar, Janja
Pergal, Marija
Piria, Marina
Paunović, Momir
Vuković-Gačić, Branka
author_sort Kolarević, Stoimir
collection PubMed
description In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg—modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg—modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).
format Online
Article
Text
id pubmed-5025182
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-50251822016-09-27 Evaluation of Genotoxic Pressure along the Sava River Kolarević, Stoimir Aborgiba, Mustafa Kračun-Kolarević, Margareta Kostić, Jovana Simonović, Predrag Simić, Vladica Milošković, Aleksandra Reischer, Georg Farnleitner, Andreas Gačić, Zoran Milačič, Radmila Zuliani, Tea Vidmar, Janja Pergal, Marija Piria, Marina Paunović, Momir Vuković-Gačić, Branka PLoS One Research Article In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg—modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg—modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples). Public Library of Science 2016-09-15 /pmc/articles/PMC5025182/ /pubmed/27631093 http://dx.doi.org/10.1371/journal.pone.0162450 Text en © 2016 Kolarević et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kolarević, Stoimir
Aborgiba, Mustafa
Kračun-Kolarević, Margareta
Kostić, Jovana
Simonović, Predrag
Simić, Vladica
Milošković, Aleksandra
Reischer, Georg
Farnleitner, Andreas
Gačić, Zoran
Milačič, Radmila
Zuliani, Tea
Vidmar, Janja
Pergal, Marija
Piria, Marina
Paunović, Momir
Vuković-Gačić, Branka
Evaluation of Genotoxic Pressure along the Sava River
title Evaluation of Genotoxic Pressure along the Sava River
title_full Evaluation of Genotoxic Pressure along the Sava River
title_fullStr Evaluation of Genotoxic Pressure along the Sava River
title_full_unstemmed Evaluation of Genotoxic Pressure along the Sava River
title_short Evaluation of Genotoxic Pressure along the Sava River
title_sort evaluation of genotoxic pressure along the sava river
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5025182/
https://www.ncbi.nlm.nih.gov/pubmed/27631093
http://dx.doi.org/10.1371/journal.pone.0162450
work_keys_str_mv AT kolarevicstoimir evaluationofgenotoxicpressurealongthesavariver
AT aborgibamustafa evaluationofgenotoxicpressurealongthesavariver
AT kracunkolarevicmargareta evaluationofgenotoxicpressurealongthesavariver
AT kosticjovana evaluationofgenotoxicpressurealongthesavariver
AT simonovicpredrag evaluationofgenotoxicpressurealongthesavariver
AT simicvladica evaluationofgenotoxicpressurealongthesavariver
AT miloskovicaleksandra evaluationofgenotoxicpressurealongthesavariver
AT reischergeorg evaluationofgenotoxicpressurealongthesavariver
AT farnleitnerandreas evaluationofgenotoxicpressurealongthesavariver
AT gaciczoran evaluationofgenotoxicpressurealongthesavariver
AT milacicradmila evaluationofgenotoxicpressurealongthesavariver
AT zulianitea evaluationofgenotoxicpressurealongthesavariver
AT vidmarjanja evaluationofgenotoxicpressurealongthesavariver
AT pergalmarija evaluationofgenotoxicpressurealongthesavariver
AT piriamarina evaluationofgenotoxicpressurealongthesavariver
AT paunovicmomir evaluationofgenotoxicpressurealongthesavariver
AT vukovicgacicbranka evaluationofgenotoxicpressurealongthesavariver