Cargando…
Deceleration of probe beam by stage bias potential improves resolution of serial block-face scanning electron microscopic images
Serial block-face scanning electron microscopy (SBEM) is quickly becoming an important imaging tool to explore three-dimensional biological structure across spatial scales. At probe-beam-electron energies of 2.0 keV or lower, the axial resolution should improve, because there is less primary electro...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5025511/ https://www.ncbi.nlm.nih.gov/pubmed/27695667 http://dx.doi.org/10.1186/s40679-016-0025-y |