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Structural Basis of Host Autophagy-related Protein 8 (ATG8) Binding by the Irish Potato Famine Pathogen Effector Protein PexRD54

Filamentous plant pathogens deliver effector proteins to host cells to promote infection. The Phytophthora infestans RXLR-type effector PexRD54 binds potato ATG8 via its ATG8 family-interacting motif (AIM) and perturbs host-selective autophagy. However, the structural basis of this interaction remai...

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Autores principales: Maqbool, Abbas, Hughes, Richard K., Dagdas, Yasin F., Tregidgo, Nicholas, Zess, Erin, Belhaj, Khaoula, Round, Adam, Bozkurt, Tolga O., Kamoun, Sophien, Banfield, Mark J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5025708/
https://www.ncbi.nlm.nih.gov/pubmed/27458016
http://dx.doi.org/10.1074/jbc.M116.744995
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author Maqbool, Abbas
Hughes, Richard K.
Dagdas, Yasin F.
Tregidgo, Nicholas
Zess, Erin
Belhaj, Khaoula
Round, Adam
Bozkurt, Tolga O.
Kamoun, Sophien
Banfield, Mark J.
author_facet Maqbool, Abbas
Hughes, Richard K.
Dagdas, Yasin F.
Tregidgo, Nicholas
Zess, Erin
Belhaj, Khaoula
Round, Adam
Bozkurt, Tolga O.
Kamoun, Sophien
Banfield, Mark J.
author_sort Maqbool, Abbas
collection PubMed
description Filamentous plant pathogens deliver effector proteins to host cells to promote infection. The Phytophthora infestans RXLR-type effector PexRD54 binds potato ATG8 via its ATG8 family-interacting motif (AIM) and perturbs host-selective autophagy. However, the structural basis of this interaction remains unknown. Here, we define the crystal structure of PexRD54, which includes a modular architecture, including five tandem repeat domains, with the AIM sequence presented at the disordered C terminus. To determine the interface between PexRD54 and ATG8, we solved the crystal structure of potato ATG8CL in complex with a peptide comprising the effector's AIM sequence, and we established a model of the full-length PexRD54-ATG8CL complex using small angle x-ray scattering. Structure-informed deletion of the PexRD54 tandem domains reveals retention of ATG8CL binding in vitro and in planta. This study offers new insights into structure/function relationships of oomycete RXLR effectors and how these proteins engage with host cell targets to promote disease.
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spelling pubmed-50257082016-09-21 Structural Basis of Host Autophagy-related Protein 8 (ATG8) Binding by the Irish Potato Famine Pathogen Effector Protein PexRD54 Maqbool, Abbas Hughes, Richard K. Dagdas, Yasin F. Tregidgo, Nicholas Zess, Erin Belhaj, Khaoula Round, Adam Bozkurt, Tolga O. Kamoun, Sophien Banfield, Mark J. J Biol Chem Papers of the Week Filamentous plant pathogens deliver effector proteins to host cells to promote infection. The Phytophthora infestans RXLR-type effector PexRD54 binds potato ATG8 via its ATG8 family-interacting motif (AIM) and perturbs host-selective autophagy. However, the structural basis of this interaction remains unknown. Here, we define the crystal structure of PexRD54, which includes a modular architecture, including five tandem repeat domains, with the AIM sequence presented at the disordered C terminus. To determine the interface between PexRD54 and ATG8, we solved the crystal structure of potato ATG8CL in complex with a peptide comprising the effector's AIM sequence, and we established a model of the full-length PexRD54-ATG8CL complex using small angle x-ray scattering. Structure-informed deletion of the PexRD54 tandem domains reveals retention of ATG8CL binding in vitro and in planta. This study offers new insights into structure/function relationships of oomycete RXLR effectors and how these proteins engage with host cell targets to promote disease. American Society for Biochemistry and Molecular Biology 2016-09-16 2016-07-25 /pmc/articles/PMC5025708/ /pubmed/27458016 http://dx.doi.org/10.1074/jbc.M116.744995 Text en © 2016 by The American Society for Biochemistry and Molecular Biology, Inc. Author's Choice—Final version free via Creative Commons CC-BY license (http://creativecommons.org/licenses/by/4.0) .
spellingShingle Papers of the Week
Maqbool, Abbas
Hughes, Richard K.
Dagdas, Yasin F.
Tregidgo, Nicholas
Zess, Erin
Belhaj, Khaoula
Round, Adam
Bozkurt, Tolga O.
Kamoun, Sophien
Banfield, Mark J.
Structural Basis of Host Autophagy-related Protein 8 (ATG8) Binding by the Irish Potato Famine Pathogen Effector Protein PexRD54
title Structural Basis of Host Autophagy-related Protein 8 (ATG8) Binding by the Irish Potato Famine Pathogen Effector Protein PexRD54
title_full Structural Basis of Host Autophagy-related Protein 8 (ATG8) Binding by the Irish Potato Famine Pathogen Effector Protein PexRD54
title_fullStr Structural Basis of Host Autophagy-related Protein 8 (ATG8) Binding by the Irish Potato Famine Pathogen Effector Protein PexRD54
title_full_unstemmed Structural Basis of Host Autophagy-related Protein 8 (ATG8) Binding by the Irish Potato Famine Pathogen Effector Protein PexRD54
title_short Structural Basis of Host Autophagy-related Protein 8 (ATG8) Binding by the Irish Potato Famine Pathogen Effector Protein PexRD54
title_sort structural basis of host autophagy-related protein 8 (atg8) binding by the irish potato famine pathogen effector protein pexrd54
topic Papers of the Week
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5025708/
https://www.ncbi.nlm.nih.gov/pubmed/27458016
http://dx.doi.org/10.1074/jbc.M116.744995
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