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Dynamics of genome change among Legionella species

Legionella species inhabit freshwater and soil ecosystems where they parasitize protozoa. L. pneumonphila (LP) serogroup-1 (Lp1) is the major cause of Legionnaires’ Disease (LD), a life-threatening pulmonary infection that can spread systemically. The increased global frequency of LD caused by Lp an...

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Autores principales: Joseph, Sandeep J., Cox, Daniel, Wolff, Bernard, Morrison, Shatavia S., Kozak-Muiznieks, Natalia A., Frace, Michael, Didelot, Xavier, Castillo-Ramirez, Santiago, Winchell, Jonas, Read, Timothy D., Dean, Deborah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5025774/
https://www.ncbi.nlm.nih.gov/pubmed/27633769
http://dx.doi.org/10.1038/srep33442
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author Joseph, Sandeep J.
Cox, Daniel
Wolff, Bernard
Morrison, Shatavia S.
Kozak-Muiznieks, Natalia A.
Frace, Michael
Didelot, Xavier
Castillo-Ramirez, Santiago
Winchell, Jonas
Read, Timothy D.
Dean, Deborah
author_facet Joseph, Sandeep J.
Cox, Daniel
Wolff, Bernard
Morrison, Shatavia S.
Kozak-Muiznieks, Natalia A.
Frace, Michael
Didelot, Xavier
Castillo-Ramirez, Santiago
Winchell, Jonas
Read, Timothy D.
Dean, Deborah
author_sort Joseph, Sandeep J.
collection PubMed
description Legionella species inhabit freshwater and soil ecosystems where they parasitize protozoa. L. pneumonphila (LP) serogroup-1 (Lp1) is the major cause of Legionnaires’ Disease (LD), a life-threatening pulmonary infection that can spread systemically. The increased global frequency of LD caused by Lp and non-Lp species underscores the need to expand our knowledge of evolutionary forces underlying disease pathogenesis. Whole genome analyses of 43 strains, including all known Lp serogroups 1–17 and 17 emergent LD-causing Legionella species (of which 33 were sequenced in this study) in addition to 10 publicly available genomes, resolved the strains into four phylogenetic clades along host virulence demarcations. Clade-specific genes were distinct for genetic exchange and signal-transduction, indicating adaptation to specific cellular and/or environmental niches. CRISPR spacer comparisons hinted at larger pools of accessory DNA sequences in Lp than predicted by the pan-genome analyses. While recombination within Lp was frequent and has been reported previously, population structure analysis identified surprisingly few DNA admixture events between species. In summary, diverse Legionella LD–causing species share a conserved core-genome, are genetically isolated from each other, and selectively acquire genes with potential for enhanced virulence.
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spelling pubmed-50257742016-09-22 Dynamics of genome change among Legionella species Joseph, Sandeep J. Cox, Daniel Wolff, Bernard Morrison, Shatavia S. Kozak-Muiznieks, Natalia A. Frace, Michael Didelot, Xavier Castillo-Ramirez, Santiago Winchell, Jonas Read, Timothy D. Dean, Deborah Sci Rep Article Legionella species inhabit freshwater and soil ecosystems where they parasitize protozoa. L. pneumonphila (LP) serogroup-1 (Lp1) is the major cause of Legionnaires’ Disease (LD), a life-threatening pulmonary infection that can spread systemically. The increased global frequency of LD caused by Lp and non-Lp species underscores the need to expand our knowledge of evolutionary forces underlying disease pathogenesis. Whole genome analyses of 43 strains, including all known Lp serogroups 1–17 and 17 emergent LD-causing Legionella species (of which 33 were sequenced in this study) in addition to 10 publicly available genomes, resolved the strains into four phylogenetic clades along host virulence demarcations. Clade-specific genes were distinct for genetic exchange and signal-transduction, indicating adaptation to specific cellular and/or environmental niches. CRISPR spacer comparisons hinted at larger pools of accessory DNA sequences in Lp than predicted by the pan-genome analyses. While recombination within Lp was frequent and has been reported previously, population structure analysis identified surprisingly few DNA admixture events between species. In summary, diverse Legionella LD–causing species share a conserved core-genome, are genetically isolated from each other, and selectively acquire genes with potential for enhanced virulence. Nature Publishing Group 2016-09-16 /pmc/articles/PMC5025774/ /pubmed/27633769 http://dx.doi.org/10.1038/srep33442 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Joseph, Sandeep J.
Cox, Daniel
Wolff, Bernard
Morrison, Shatavia S.
Kozak-Muiznieks, Natalia A.
Frace, Michael
Didelot, Xavier
Castillo-Ramirez, Santiago
Winchell, Jonas
Read, Timothy D.
Dean, Deborah
Dynamics of genome change among Legionella species
title Dynamics of genome change among Legionella species
title_full Dynamics of genome change among Legionella species
title_fullStr Dynamics of genome change among Legionella species
title_full_unstemmed Dynamics of genome change among Legionella species
title_short Dynamics of genome change among Legionella species
title_sort dynamics of genome change among legionella species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5025774/
https://www.ncbi.nlm.nih.gov/pubmed/27633769
http://dx.doi.org/10.1038/srep33442
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