Cargando…
Intestinal microbiota in pediatric patients with end stage renal disease: a Midwest Pediatric Nephrology Consortium study
BACKGROUND: End-stage renal disease (ESRD) is associated with uremia and increased systemic inflammation. Alteration of the intestinal microbiota may facilitate translocation of endotoxins into the systemic circulation leading to inflammation. We hypothesized that children with ESRD have an altered...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027112/ https://www.ncbi.nlm.nih.gov/pubmed/27640125 http://dx.doi.org/10.1186/s40168-016-0195-9 |
_version_ | 1782454187960827904 |
---|---|
author | Crespo-Salgado, Janice Vehaskari, V. Matti Stewart, Tyrus Ferris, Michael Zhang, Qiang Wang, Guangdi Blanchard, Eugene E. Taylor, Christopher M. Kallash, Mahmoud Greenbaum, Larry A. Aviles, Diego H. |
author_facet | Crespo-Salgado, Janice Vehaskari, V. Matti Stewart, Tyrus Ferris, Michael Zhang, Qiang Wang, Guangdi Blanchard, Eugene E. Taylor, Christopher M. Kallash, Mahmoud Greenbaum, Larry A. Aviles, Diego H. |
author_sort | Crespo-Salgado, Janice |
collection | PubMed |
description | BACKGROUND: End-stage renal disease (ESRD) is associated with uremia and increased systemic inflammation. Alteration of the intestinal microbiota may facilitate translocation of endotoxins into the systemic circulation leading to inflammation. We hypothesized that children with ESRD have an altered intestinal microbiota and increased serum levels of bacterially derived uremic toxins. METHODS: Four groups of subjects were recruited: peritoneal dialysis (PD), hemodialysis (HD), post-kidney transplant and healthy controls. Stool bacterial composition was assessed by pyrosequencing analysis of 16S rRNA genes. Serum levels of C-reactive protein (CRP), D-lactate, p-cresyl sulfate and indoxyl sulfate were measured. RESULTS: Compared to controls, the relative abundance of Firmicutes (P = 0.0228) and Actinobacteria (P = 0.0040) was decreased in PD patients. The relative abundance of Bacteroidetes was increased in HD patients (P = 0.0462). Compared to HD patients the relative abundance of Proteobacteria (P = 0.0233) was increased in PD patients. At the family level, Enterobacteriaceae was significantly increased in PD patients (P = 0.0020) compared to controls; whereas, Bifidobacteria showed a significant decrease in PD and transplant patients (P = 0.0020) compared to control. Alpha diversity was decreased in PD patients and kidney transplant using both phylogenetic and non-phylogenetic diversity measures (P = 0.0031 and 0.0003, respectively), while beta diversity showed significant separation (R statistic = 0.2656, P = 0.010) between PD patients and controls. ESRD patients had increased serum levels of p-cresyl sulfate and indoxyl sulfate (P < 0.0001 and P < 0.0001, respectively). The data suggests that no significant correlation exists between the alpha diversity of the intestinal microbiota and CRP, D-lactate, or uremic toxins. Oral iron supplementation results in expansion of the phylum Proteobacteria. CONCLUSIONS: Children with ESRD have altered intestinal microbiota and increased bacterially derived serum uremic toxins. |
format | Online Article Text |
id | pubmed-5027112 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-50271122016-09-22 Intestinal microbiota in pediatric patients with end stage renal disease: a Midwest Pediatric Nephrology Consortium study Crespo-Salgado, Janice Vehaskari, V. Matti Stewart, Tyrus Ferris, Michael Zhang, Qiang Wang, Guangdi Blanchard, Eugene E. Taylor, Christopher M. Kallash, Mahmoud Greenbaum, Larry A. Aviles, Diego H. Microbiome Research BACKGROUND: End-stage renal disease (ESRD) is associated with uremia and increased systemic inflammation. Alteration of the intestinal microbiota may facilitate translocation of endotoxins into the systemic circulation leading to inflammation. We hypothesized that children with ESRD have an altered intestinal microbiota and increased serum levels of bacterially derived uremic toxins. METHODS: Four groups of subjects were recruited: peritoneal dialysis (PD), hemodialysis (HD), post-kidney transplant and healthy controls. Stool bacterial composition was assessed by pyrosequencing analysis of 16S rRNA genes. Serum levels of C-reactive protein (CRP), D-lactate, p-cresyl sulfate and indoxyl sulfate were measured. RESULTS: Compared to controls, the relative abundance of Firmicutes (P = 0.0228) and Actinobacteria (P = 0.0040) was decreased in PD patients. The relative abundance of Bacteroidetes was increased in HD patients (P = 0.0462). Compared to HD patients the relative abundance of Proteobacteria (P = 0.0233) was increased in PD patients. At the family level, Enterobacteriaceae was significantly increased in PD patients (P = 0.0020) compared to controls; whereas, Bifidobacteria showed a significant decrease in PD and transplant patients (P = 0.0020) compared to control. Alpha diversity was decreased in PD patients and kidney transplant using both phylogenetic and non-phylogenetic diversity measures (P = 0.0031 and 0.0003, respectively), while beta diversity showed significant separation (R statistic = 0.2656, P = 0.010) between PD patients and controls. ESRD patients had increased serum levels of p-cresyl sulfate and indoxyl sulfate (P < 0.0001 and P < 0.0001, respectively). The data suggests that no significant correlation exists between the alpha diversity of the intestinal microbiota and CRP, D-lactate, or uremic toxins. Oral iron supplementation results in expansion of the phylum Proteobacteria. CONCLUSIONS: Children with ESRD have altered intestinal microbiota and increased bacterially derived serum uremic toxins. BioMed Central 2016-09-17 /pmc/articles/PMC5027112/ /pubmed/27640125 http://dx.doi.org/10.1186/s40168-016-0195-9 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Crespo-Salgado, Janice Vehaskari, V. Matti Stewart, Tyrus Ferris, Michael Zhang, Qiang Wang, Guangdi Blanchard, Eugene E. Taylor, Christopher M. Kallash, Mahmoud Greenbaum, Larry A. Aviles, Diego H. Intestinal microbiota in pediatric patients with end stage renal disease: a Midwest Pediatric Nephrology Consortium study |
title | Intestinal microbiota in pediatric patients with end stage renal disease: a Midwest Pediatric Nephrology Consortium study |
title_full | Intestinal microbiota in pediatric patients with end stage renal disease: a Midwest Pediatric Nephrology Consortium study |
title_fullStr | Intestinal microbiota in pediatric patients with end stage renal disease: a Midwest Pediatric Nephrology Consortium study |
title_full_unstemmed | Intestinal microbiota in pediatric patients with end stage renal disease: a Midwest Pediatric Nephrology Consortium study |
title_short | Intestinal microbiota in pediatric patients with end stage renal disease: a Midwest Pediatric Nephrology Consortium study |
title_sort | intestinal microbiota in pediatric patients with end stage renal disease: a midwest pediatric nephrology consortium study |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027112/ https://www.ncbi.nlm.nih.gov/pubmed/27640125 http://dx.doi.org/10.1186/s40168-016-0195-9 |
work_keys_str_mv | AT cresposalgadojanice intestinalmicrobiotainpediatricpatientswithendstagerenaldiseaseamidwestpediatricnephrologyconsortiumstudy AT vehaskarivmatti intestinalmicrobiotainpediatricpatientswithendstagerenaldiseaseamidwestpediatricnephrologyconsortiumstudy AT stewarttyrus intestinalmicrobiotainpediatricpatientswithendstagerenaldiseaseamidwestpediatricnephrologyconsortiumstudy AT ferrismichael intestinalmicrobiotainpediatricpatientswithendstagerenaldiseaseamidwestpediatricnephrologyconsortiumstudy AT zhangqiang intestinalmicrobiotainpediatricpatientswithendstagerenaldiseaseamidwestpediatricnephrologyconsortiumstudy AT wangguangdi intestinalmicrobiotainpediatricpatientswithendstagerenaldiseaseamidwestpediatricnephrologyconsortiumstudy AT blanchardeugenee intestinalmicrobiotainpediatricpatientswithendstagerenaldiseaseamidwestpediatricnephrologyconsortiumstudy AT taylorchristopherm intestinalmicrobiotainpediatricpatientswithendstagerenaldiseaseamidwestpediatricnephrologyconsortiumstudy AT kallashmahmoud intestinalmicrobiotainpediatricpatientswithendstagerenaldiseaseamidwestpediatricnephrologyconsortiumstudy AT greenbaumlarrya intestinalmicrobiotainpediatricpatientswithendstagerenaldiseaseamidwestpediatricnephrologyconsortiumstudy AT avilesdiegoh intestinalmicrobiotainpediatricpatientswithendstagerenaldiseaseamidwestpediatricnephrologyconsortiumstudy |