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Regulatory SNPs Alter the Gene Expression of Diabetic Retinopathy Associated Secretary Factors

Objectives: Diabetic retinopathy (DR) is a common microvascular complication in both type I and type II diabetes. Several previous reports indicated the serum centration of some secretary factors were highly associated with DR. Therefore, we hypothesis regulatory SNPs (rSNPs) genotype in secretary f...

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Autores principales: Chen, Chian-Feng, Liou, Shiow-Wen, Wu, Hsin-Han, Lin, Chin-Hui, Huang, Li-Shan, Woung, Lin-Chung, Tsai, Ching-Yao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027191/
https://www.ncbi.nlm.nih.gov/pubmed/27648002
http://dx.doi.org/10.7150/ijms.16345
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author Chen, Chian-Feng
Liou, Shiow-Wen
Wu, Hsin-Han
Lin, Chin-Hui
Huang, Li-Shan
Woung, Lin-Chung
Tsai, Ching-Yao
author_facet Chen, Chian-Feng
Liou, Shiow-Wen
Wu, Hsin-Han
Lin, Chin-Hui
Huang, Li-Shan
Woung, Lin-Chung
Tsai, Ching-Yao
author_sort Chen, Chian-Feng
collection PubMed
description Objectives: Diabetic retinopathy (DR) is a common microvascular complication in both type I and type II diabetes. Several previous reports indicated the serum centration of some secretary factors were highly associated with DR. Therefore, we hypothesis regulatory SNPs (rSNPs) genotype in secretary factors may alter these gene expression and lead to DR. Methods: At first, pyrosequencing were applying to screen the SNPs which present allele frequency different in DR and DNR. Then individual genotyping was processed by Taqman assays in Taiwanese DR and DNR patients. To evaluate the effect of SNP allele on transcriptional activity, we measured promoter activity using luciferase reporter constructs. Results: We found the frequencies of the CC, CG, and GG genotype of the rs2010963 polymorphism were 15.09%, 47.14%, and 37.74% in DR and 12.90%, 19.35%, and 67.74% in DNR, respectively (p = 0.0205). The prevalence of DR was higher (p = 0.00793) in patients with the CC or CG genotype (62.26% and 32.26% for DR and DNR, respectively) compared with the patients with the GG genotype. To evaluate the effect of rs2010963-C allele on transcriptional activity, we measured promoter activity using luciferase reporter constructs. The rs2010963-C reporter showed 1.6 to 2-fold higher luciferase activity than rs2010963-G in 3 cell lines. Conclusion: Our data proposed rs2010963-C altered the expression level of VEGFA in different tissues. We suggested small increase but long term exposure to VEGFA may lead to DR finally.
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spelling pubmed-50271912016-09-19 Regulatory SNPs Alter the Gene Expression of Diabetic Retinopathy Associated Secretary Factors Chen, Chian-Feng Liou, Shiow-Wen Wu, Hsin-Han Lin, Chin-Hui Huang, Li-Shan Woung, Lin-Chung Tsai, Ching-Yao Int J Med Sci Research Paper Objectives: Diabetic retinopathy (DR) is a common microvascular complication in both type I and type II diabetes. Several previous reports indicated the serum centration of some secretary factors were highly associated with DR. Therefore, we hypothesis regulatory SNPs (rSNPs) genotype in secretary factors may alter these gene expression and lead to DR. Methods: At first, pyrosequencing were applying to screen the SNPs which present allele frequency different in DR and DNR. Then individual genotyping was processed by Taqman assays in Taiwanese DR and DNR patients. To evaluate the effect of SNP allele on transcriptional activity, we measured promoter activity using luciferase reporter constructs. Results: We found the frequencies of the CC, CG, and GG genotype of the rs2010963 polymorphism were 15.09%, 47.14%, and 37.74% in DR and 12.90%, 19.35%, and 67.74% in DNR, respectively (p = 0.0205). The prevalence of DR was higher (p = 0.00793) in patients with the CC or CG genotype (62.26% and 32.26% for DR and DNR, respectively) compared with the patients with the GG genotype. To evaluate the effect of rs2010963-C allele on transcriptional activity, we measured promoter activity using luciferase reporter constructs. The rs2010963-C reporter showed 1.6 to 2-fold higher luciferase activity than rs2010963-G in 3 cell lines. Conclusion: Our data proposed rs2010963-C altered the expression level of VEGFA in different tissues. We suggested small increase but long term exposure to VEGFA may lead to DR finally. Ivyspring International Publisher 2016-09-12 /pmc/articles/PMC5027191/ /pubmed/27648002 http://dx.doi.org/10.7150/ijms.16345 Text en © Ivyspring International Publisher. Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. See http://ivyspring.com/terms for terms and conditions.
spellingShingle Research Paper
Chen, Chian-Feng
Liou, Shiow-Wen
Wu, Hsin-Han
Lin, Chin-Hui
Huang, Li-Shan
Woung, Lin-Chung
Tsai, Ching-Yao
Regulatory SNPs Alter the Gene Expression of Diabetic Retinopathy Associated Secretary Factors
title Regulatory SNPs Alter the Gene Expression of Diabetic Retinopathy Associated Secretary Factors
title_full Regulatory SNPs Alter the Gene Expression of Diabetic Retinopathy Associated Secretary Factors
title_fullStr Regulatory SNPs Alter the Gene Expression of Diabetic Retinopathy Associated Secretary Factors
title_full_unstemmed Regulatory SNPs Alter the Gene Expression of Diabetic Retinopathy Associated Secretary Factors
title_short Regulatory SNPs Alter the Gene Expression of Diabetic Retinopathy Associated Secretary Factors
title_sort regulatory snps alter the gene expression of diabetic retinopathy associated secretary factors
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027191/
https://www.ncbi.nlm.nih.gov/pubmed/27648002
http://dx.doi.org/10.7150/ijms.16345
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