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Mice produced by mitotic reprogramming of sperm injected into haploid parthenogenotes

Sperm are highly differentiated and the activities that reprogram them for embryonic development during fertilization have historically been considered unique to the oocyte. We here challenge this view and demonstrate that mouse embryos in the mitotic cell cycle can also directly reprogram sperm for...

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Autores principales: Suzuki, Toru, Asami, Maki, Hoffmann, Martin, Lu, Xin, Gužvić, Miodrag, Klein, Christoph A., Perry, Anthony C. F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027272/
https://www.ncbi.nlm.nih.gov/pubmed/27623537
http://dx.doi.org/10.1038/ncomms12676
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author Suzuki, Toru
Asami, Maki
Hoffmann, Martin
Lu, Xin
Gužvić, Miodrag
Klein, Christoph A.
Perry, Anthony C. F.
author_facet Suzuki, Toru
Asami, Maki
Hoffmann, Martin
Lu, Xin
Gužvić, Miodrag
Klein, Christoph A.
Perry, Anthony C. F.
author_sort Suzuki, Toru
collection PubMed
description Sperm are highly differentiated and the activities that reprogram them for embryonic development during fertilization have historically been considered unique to the oocyte. We here challenge this view and demonstrate that mouse embryos in the mitotic cell cycle can also directly reprogram sperm for full-term development. Developmentally incompetent haploid embryos (parthenogenotes) injected with sperm developed to produce healthy offspring at up to 24% of control rates, depending when in the embryonic cell cycle injection took place. This implies that most of the first embryonic cell cycle can be bypassed in sperm genome reprogramming for full development. Remodelling of histones and genomic 5′-methylcytosine and 5′-hydroxymethylcytosine following embryo injection were distinct from remodelling in fertilization and the resulting 2-cell embryos consistently possessed abnormal transcriptomes. These studies demonstrate plasticity in the reprogramming of terminally differentiated sperm nuclei and suggest that different epigenetic pathways or kinetics can establish totipotency.
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spelling pubmed-50272722016-09-23 Mice produced by mitotic reprogramming of sperm injected into haploid parthenogenotes Suzuki, Toru Asami, Maki Hoffmann, Martin Lu, Xin Gužvić, Miodrag Klein, Christoph A. Perry, Anthony C. F. Nat Commun Article Sperm are highly differentiated and the activities that reprogram them for embryonic development during fertilization have historically been considered unique to the oocyte. We here challenge this view and demonstrate that mouse embryos in the mitotic cell cycle can also directly reprogram sperm for full-term development. Developmentally incompetent haploid embryos (parthenogenotes) injected with sperm developed to produce healthy offspring at up to 24% of control rates, depending when in the embryonic cell cycle injection took place. This implies that most of the first embryonic cell cycle can be bypassed in sperm genome reprogramming for full development. Remodelling of histones and genomic 5′-methylcytosine and 5′-hydroxymethylcytosine following embryo injection were distinct from remodelling in fertilization and the resulting 2-cell embryos consistently possessed abnormal transcriptomes. These studies demonstrate plasticity in the reprogramming of terminally differentiated sperm nuclei and suggest that different epigenetic pathways or kinetics can establish totipotency. Nature Publishing Group 2016-09-13 /pmc/articles/PMC5027272/ /pubmed/27623537 http://dx.doi.org/10.1038/ncomms12676 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Suzuki, Toru
Asami, Maki
Hoffmann, Martin
Lu, Xin
Gužvić, Miodrag
Klein, Christoph A.
Perry, Anthony C. F.
Mice produced by mitotic reprogramming of sperm injected into haploid parthenogenotes
title Mice produced by mitotic reprogramming of sperm injected into haploid parthenogenotes
title_full Mice produced by mitotic reprogramming of sperm injected into haploid parthenogenotes
title_fullStr Mice produced by mitotic reprogramming of sperm injected into haploid parthenogenotes
title_full_unstemmed Mice produced by mitotic reprogramming of sperm injected into haploid parthenogenotes
title_short Mice produced by mitotic reprogramming of sperm injected into haploid parthenogenotes
title_sort mice produced by mitotic reprogramming of sperm injected into haploid parthenogenotes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027272/
https://www.ncbi.nlm.nih.gov/pubmed/27623537
http://dx.doi.org/10.1038/ncomms12676
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