Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9

Binding specificity of Cas9–guide RNA complexes to DNA is important for genome-engineering applications; however, how mismatches influence target recognition/rejection kinetics is not well understood. Here we used single-molecule FRET to probe real-time interactions between Cas9–RNA and DNA targets....

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Detalles Bibliográficos
Autores principales: Singh, Digvijay, Sternberg, Samuel H., Fei, Jingyi, Doudna, Jennifer A., Ha, Taekjip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027287/
https://www.ncbi.nlm.nih.gov/pubmed/27624851
http://dx.doi.org/10.1038/ncomms12778

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