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Angiogenic Effects of Collagen/Mesoporous Nanoparticle Composite Scaffold Delivering VEGF(165)

Vascularization is a key issue for the success of tissue engineering to repair damaged tissue. In this study, we report a composite scaffold delivering angiogenic factor for this purpose. Vascular endothelial growth factor (VEGF) was loaded on mesoporous silica nanoparticle (MSN), which was then inc...

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Detalles Bibliográficos
Autores principales: Kim, Joong-Hyun, Kim, Tae-Hyun, Kang, Min Sil, Kim, Hae-Won
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027316/
https://www.ncbi.nlm.nih.gov/pubmed/27689093
http://dx.doi.org/10.1155/2016/9676934
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author Kim, Joong-Hyun
Kim, Tae-Hyun
Kang, Min Sil
Kim, Hae-Won
author_facet Kim, Joong-Hyun
Kim, Tae-Hyun
Kang, Min Sil
Kim, Hae-Won
author_sort Kim, Joong-Hyun
collection PubMed
description Vascularization is a key issue for the success of tissue engineering to repair damaged tissue. In this study, we report a composite scaffold delivering angiogenic factor for this purpose. Vascular endothelial growth factor (VEGF) was loaded on mesoporous silica nanoparticle (MSN), which was then incorporated within a type I collagen sponge, to produce collagen/MSN/VEGF (CMV) scaffold. The CMV composite scaffold could release VEGF sustainably over the test period of 28 days. The release of VEGF improved the cell proliferation. Moreover, the in vivo angiogenesis of the scaffold, as studied by the chick chorioallantoic membrane (CAM) model, showed that the VEGF-releasing scaffold induced significantly increased number of blood vessel complexes when compared with VEGF-free scaffold. The composite scaffold showed good biocompatibility, as examined in rat subcutaneous tissue. These results demonstrate that the CMV scaffold with VEGF-releasing capacity can be potentially used to stimulate angiogenesis and tissue repair.
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spelling pubmed-50273162016-09-29 Angiogenic Effects of Collagen/Mesoporous Nanoparticle Composite Scaffold Delivering VEGF(165) Kim, Joong-Hyun Kim, Tae-Hyun Kang, Min Sil Kim, Hae-Won Biomed Res Int Research Article Vascularization is a key issue for the success of tissue engineering to repair damaged tissue. In this study, we report a composite scaffold delivering angiogenic factor for this purpose. Vascular endothelial growth factor (VEGF) was loaded on mesoporous silica nanoparticle (MSN), which was then incorporated within a type I collagen sponge, to produce collagen/MSN/VEGF (CMV) scaffold. The CMV composite scaffold could release VEGF sustainably over the test period of 28 days. The release of VEGF improved the cell proliferation. Moreover, the in vivo angiogenesis of the scaffold, as studied by the chick chorioallantoic membrane (CAM) model, showed that the VEGF-releasing scaffold induced significantly increased number of blood vessel complexes when compared with VEGF-free scaffold. The composite scaffold showed good biocompatibility, as examined in rat subcutaneous tissue. These results demonstrate that the CMV scaffold with VEGF-releasing capacity can be potentially used to stimulate angiogenesis and tissue repair. Hindawi Publishing Corporation 2016 2016-09-05 /pmc/articles/PMC5027316/ /pubmed/27689093 http://dx.doi.org/10.1155/2016/9676934 Text en Copyright © 2016 Joong-Hyun Kim et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kim, Joong-Hyun
Kim, Tae-Hyun
Kang, Min Sil
Kim, Hae-Won
Angiogenic Effects of Collagen/Mesoporous Nanoparticle Composite Scaffold Delivering VEGF(165)
title Angiogenic Effects of Collagen/Mesoporous Nanoparticle Composite Scaffold Delivering VEGF(165)
title_full Angiogenic Effects of Collagen/Mesoporous Nanoparticle Composite Scaffold Delivering VEGF(165)
title_fullStr Angiogenic Effects of Collagen/Mesoporous Nanoparticle Composite Scaffold Delivering VEGF(165)
title_full_unstemmed Angiogenic Effects of Collagen/Mesoporous Nanoparticle Composite Scaffold Delivering VEGF(165)
title_short Angiogenic Effects of Collagen/Mesoporous Nanoparticle Composite Scaffold Delivering VEGF(165)
title_sort angiogenic effects of collagen/mesoporous nanoparticle composite scaffold delivering vegf(165)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027316/
https://www.ncbi.nlm.nih.gov/pubmed/27689093
http://dx.doi.org/10.1155/2016/9676934
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