Transition from physical activity to inactivity increases skeletal muscle miR‐148b content and triggers insulin resistance

This study investigated miR‐148b as a potential physiological actor of physical inactivity‐induced effects in skeletal muscle. By using animal and human protocols, we demonstrated that the early phase of transition toward inactivity was associated with an increase in muscle miR‐148b content, which triggered the downregulation of NRAS and ROCK1 target genes. Using human myotubes, we demonstrated that overexpression of miR‐148b decreased NRAS and ROCK1 protein levels, and PKB phosphorylation and glucose uptake in response to insulin. Increase in muscle miR‐148b content might thus participate in the decrease in insulin sensitivity at the whole body level during the transition toward physical inactivity.