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Saturated high‐fat diet‐induced obesity increases adenylate cyclase of myocardial β‐adrenergic system and does not compromise cardiac function

Obesity is a worldwide pandemic associated with high incidence of cardiovascular disease. The mechanisms by which the obesity leads cardiac dysfunction are not fully elucidated and few studies have evaluated the relationship between obesity and proteins involved in myocardial β‐adrenergic (βA) syste...

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Autores principales: Vileigas, Danielle F., de Deus, Adriana F., da Silva, Danielle C. T., de Tomasi, Loreta C., de Campos, Dijon H. S., Adorni, Caroline S., de Oliveira, Scarlet M., Sant'Ana, Paula G., Okoshi, Katashi, Padovani, Carlos R., Cicogna, Antonio C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027348/
https://www.ncbi.nlm.nih.gov/pubmed/27582064
http://dx.doi.org/10.14814/phy2.12914
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author Vileigas, Danielle F.
de Deus, Adriana F.
da Silva, Danielle C. T.
de Tomasi, Loreta C.
de Campos, Dijon H. S.
Adorni, Caroline S.
de Oliveira, Scarlet M.
Sant'Ana, Paula G.
Okoshi, Katashi
Padovani, Carlos R.
Cicogna, Antonio C.
author_facet Vileigas, Danielle F.
de Deus, Adriana F.
da Silva, Danielle C. T.
de Tomasi, Loreta C.
de Campos, Dijon H. S.
Adorni, Caroline S.
de Oliveira, Scarlet M.
Sant'Ana, Paula G.
Okoshi, Katashi
Padovani, Carlos R.
Cicogna, Antonio C.
author_sort Vileigas, Danielle F.
collection PubMed
description Obesity is a worldwide pandemic associated with high incidence of cardiovascular disease. The mechanisms by which the obesity leads cardiac dysfunction are not fully elucidated and few studies have evaluated the relationship between obesity and proteins involved in myocardial β‐adrenergic (βA) system. The purpose of this study was to evaluate the cardiac function and βA pathway components in myocardium of obese rats. Male Wistar rats were distributed into two groups: control (n = 17; standard diet) and obese (n = 17; saturated high‐fat diet) fed for 33 weeks. Nutritional profile and comorbidities were assessed. Cardiac structure and function was evaluated by macroscopic postmortem, echocardiographic and isolated papillary muscle analyzes. Myocardial protein expression of β (1)‐ and β (2)‐adrenergic receptors, Gαs protein, adenylate cyclase (AC) and protein kinase A (PKA) was performed by Western blot. Cardiac cyclic adenosine monophosphate (cAMP) levels and PKA activity were assessed by ELISA. Obese rats showed increased adiposity index (P < 0.001) and several comorbidities as hypertension, glucose intolerance, insulin resistance, and dyslipidemia compared with control rats. Echocardiographic assessment revealed increased left atrium diameter (C: 4.98 ± 0.38 vs. Ob: 5.47 ± 0.53, P = 0.024) and posterior wall shortening velocity (C: 37.1 ± 3.6 vs. Ob: 41.8 ± 3.8, P = 0.007) in obese group. Papillary muscle evaluation indicated that baseline data and myocardial responsiveness to isoproterenol stimulation were similar between the groups. Protein expression of myocardial AC was higher in obese group than in the control (C: 1.00 ± 0.21 vs. Ob: 1.25 ± 0.10, P = 0.025), whereas the other components were unchanged. These results suggest that saturated high‐fat diet‐induced obesity was not effective in triggering cardiac dysfunction and impair the beta‐adrenergic signaling.
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spelling pubmed-50273482017-03-07 Saturated high‐fat diet‐induced obesity increases adenylate cyclase of myocardial β‐adrenergic system and does not compromise cardiac function Vileigas, Danielle F. de Deus, Adriana F. da Silva, Danielle C. T. de Tomasi, Loreta C. de Campos, Dijon H. S. Adorni, Caroline S. de Oliveira, Scarlet M. Sant'Ana, Paula G. Okoshi, Katashi Padovani, Carlos R. Cicogna, Antonio C. Physiol Rep Original Research Obesity is a worldwide pandemic associated with high incidence of cardiovascular disease. The mechanisms by which the obesity leads cardiac dysfunction are not fully elucidated and few studies have evaluated the relationship between obesity and proteins involved in myocardial β‐adrenergic (βA) system. The purpose of this study was to evaluate the cardiac function and βA pathway components in myocardium of obese rats. Male Wistar rats were distributed into two groups: control (n = 17; standard diet) and obese (n = 17; saturated high‐fat diet) fed for 33 weeks. Nutritional profile and comorbidities were assessed. Cardiac structure and function was evaluated by macroscopic postmortem, echocardiographic and isolated papillary muscle analyzes. Myocardial protein expression of β (1)‐ and β (2)‐adrenergic receptors, Gαs protein, adenylate cyclase (AC) and protein kinase A (PKA) was performed by Western blot. Cardiac cyclic adenosine monophosphate (cAMP) levels and PKA activity were assessed by ELISA. Obese rats showed increased adiposity index (P < 0.001) and several comorbidities as hypertension, glucose intolerance, insulin resistance, and dyslipidemia compared with control rats. Echocardiographic assessment revealed increased left atrium diameter (C: 4.98 ± 0.38 vs. Ob: 5.47 ± 0.53, P = 0.024) and posterior wall shortening velocity (C: 37.1 ± 3.6 vs. Ob: 41.8 ± 3.8, P = 0.007) in obese group. Papillary muscle evaluation indicated that baseline data and myocardial responsiveness to isoproterenol stimulation were similar between the groups. Protein expression of myocardial AC was higher in obese group than in the control (C: 1.00 ± 0.21 vs. Ob: 1.25 ± 0.10, P = 0.025), whereas the other components were unchanged. These results suggest that saturated high‐fat diet‐induced obesity was not effective in triggering cardiac dysfunction and impair the beta‐adrenergic signaling. John Wiley and Sons Inc. 2016-08-31 /pmc/articles/PMC5027348/ /pubmed/27582064 http://dx.doi.org/10.14814/phy2.12914 Text en © 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Vileigas, Danielle F.
de Deus, Adriana F.
da Silva, Danielle C. T.
de Tomasi, Loreta C.
de Campos, Dijon H. S.
Adorni, Caroline S.
de Oliveira, Scarlet M.
Sant'Ana, Paula G.
Okoshi, Katashi
Padovani, Carlos R.
Cicogna, Antonio C.
Saturated high‐fat diet‐induced obesity increases adenylate cyclase of myocardial β‐adrenergic system and does not compromise cardiac function
title Saturated high‐fat diet‐induced obesity increases adenylate cyclase of myocardial β‐adrenergic system and does not compromise cardiac function
title_full Saturated high‐fat diet‐induced obesity increases adenylate cyclase of myocardial β‐adrenergic system and does not compromise cardiac function
title_fullStr Saturated high‐fat diet‐induced obesity increases adenylate cyclase of myocardial β‐adrenergic system and does not compromise cardiac function
title_full_unstemmed Saturated high‐fat diet‐induced obesity increases adenylate cyclase of myocardial β‐adrenergic system and does not compromise cardiac function
title_short Saturated high‐fat diet‐induced obesity increases adenylate cyclase of myocardial β‐adrenergic system and does not compromise cardiac function
title_sort saturated high‐fat diet‐induced obesity increases adenylate cyclase of myocardial β‐adrenergic system and does not compromise cardiac function
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027348/
https://www.ncbi.nlm.nih.gov/pubmed/27582064
http://dx.doi.org/10.14814/phy2.12914
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