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Turning the fate of reprogramming cells from retinal disorder to regeneration by Pax6 in newts
The newt, a urodele amphibian, has an outstanding ability– even as an adult –to regenerate a functional retina through reprogramming and proliferation of the retinal pigment epithelium (RPE) cells, even though the neural retina is completely removed from the eye by surgery. It remains unknown how th...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027390/ https://www.ncbi.nlm.nih.gov/pubmed/27640672 http://dx.doi.org/10.1038/srep33761 |
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author | Casco-Robles, Martin Miguel Islam, Md Rafiqul Inami, Wataru Tanaka, Hibiki Vincent Kunahong, Ailidana Yasumuro, Hirofumi Hanzawa, Shiori Casco-Robles, Roman Martin Toyama, Fubito Maruo, Fumiaki Chiba, Chikafumi |
author_facet | Casco-Robles, Martin Miguel Islam, Md Rafiqul Inami, Wataru Tanaka, Hibiki Vincent Kunahong, Ailidana Yasumuro, Hirofumi Hanzawa, Shiori Casco-Robles, Roman Martin Toyama, Fubito Maruo, Fumiaki Chiba, Chikafumi |
author_sort | Casco-Robles, Martin Miguel |
collection | PubMed |
description | The newt, a urodele amphibian, has an outstanding ability– even as an adult –to regenerate a functional retina through reprogramming and proliferation of the retinal pigment epithelium (RPE) cells, even though the neural retina is completely removed from the eye by surgery. It remains unknown how the newt invented such a superior mechanism. Here we show that disability of RPE cells to regenerate the retina brings about a symptom of proliferative vitreoretinopathy (PVR), even in the newt. When Pax6, a transcription factor that is re-expressed in reprogramming RPE cells, is knocked down in transgenic juvenile newts, these cells proliferate but eventually give rise to cell aggregates that uniformly express alpha smooth muscle actin, Vimentin and N-cadherin, the markers of myofibroblasts which are a major component of the sub-/epi-retinal membranes in PVR. Our current study demonstrates that Pax6 is an essential factor that directs the fate of reprogramming RPE cells toward the retinal regeneration. The newt may have evolved the ability of retinal regeneration by modifying a mechanism that underlies the RPE-mediated retinal disorders. |
format | Online Article Text |
id | pubmed-5027390 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50273902016-09-22 Turning the fate of reprogramming cells from retinal disorder to regeneration by Pax6 in newts Casco-Robles, Martin Miguel Islam, Md Rafiqul Inami, Wataru Tanaka, Hibiki Vincent Kunahong, Ailidana Yasumuro, Hirofumi Hanzawa, Shiori Casco-Robles, Roman Martin Toyama, Fubito Maruo, Fumiaki Chiba, Chikafumi Sci Rep Article The newt, a urodele amphibian, has an outstanding ability– even as an adult –to regenerate a functional retina through reprogramming and proliferation of the retinal pigment epithelium (RPE) cells, even though the neural retina is completely removed from the eye by surgery. It remains unknown how the newt invented such a superior mechanism. Here we show that disability of RPE cells to regenerate the retina brings about a symptom of proliferative vitreoretinopathy (PVR), even in the newt. When Pax6, a transcription factor that is re-expressed in reprogramming RPE cells, is knocked down in transgenic juvenile newts, these cells proliferate but eventually give rise to cell aggregates that uniformly express alpha smooth muscle actin, Vimentin and N-cadherin, the markers of myofibroblasts which are a major component of the sub-/epi-retinal membranes in PVR. Our current study demonstrates that Pax6 is an essential factor that directs the fate of reprogramming RPE cells toward the retinal regeneration. The newt may have evolved the ability of retinal regeneration by modifying a mechanism that underlies the RPE-mediated retinal disorders. Nature Publishing Group 2016-09-19 /pmc/articles/PMC5027390/ /pubmed/27640672 http://dx.doi.org/10.1038/srep33761 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Casco-Robles, Martin Miguel Islam, Md Rafiqul Inami, Wataru Tanaka, Hibiki Vincent Kunahong, Ailidana Yasumuro, Hirofumi Hanzawa, Shiori Casco-Robles, Roman Martin Toyama, Fubito Maruo, Fumiaki Chiba, Chikafumi Turning the fate of reprogramming cells from retinal disorder to regeneration by Pax6 in newts |
title | Turning the fate of reprogramming cells from retinal disorder to regeneration by Pax6 in newts |
title_full | Turning the fate of reprogramming cells from retinal disorder to regeneration by Pax6 in newts |
title_fullStr | Turning the fate of reprogramming cells from retinal disorder to regeneration by Pax6 in newts |
title_full_unstemmed | Turning the fate of reprogramming cells from retinal disorder to regeneration by Pax6 in newts |
title_short | Turning the fate of reprogramming cells from retinal disorder to regeneration by Pax6 in newts |
title_sort | turning the fate of reprogramming cells from retinal disorder to regeneration by pax6 in newts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027390/ https://www.ncbi.nlm.nih.gov/pubmed/27640672 http://dx.doi.org/10.1038/srep33761 |
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