The coming paradigm shift: A transition from manual to automated microscopy
The field of pathology has used light microscopy (LM) extensively since the mid-19(th) century for examination of histological tissue preparations. This technology has remained the foremost tool in use by pathologists even as other fields have undergone a great change in recent years through new tec...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Medknow Publications & Media Pvt Ltd
2016
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027735/ https://www.ncbi.nlm.nih.gov/pubmed/27688926 http://dx.doi.org/10.4103/2153-3539.189698 |
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author | Farahani, Navid Monteith, Corey E. |
author_facet | Farahani, Navid Monteith, Corey E. |
author_sort | Farahani, Navid |
collection | PubMed |
description | The field of pathology has used light microscopy (LM) extensively since the mid-19(th) century for examination of histological tissue preparations. This technology has remained the foremost tool in use by pathologists even as other fields have undergone a great change in recent years through new technologies. However, as new microscopy techniques are perfected and made available, this reliance on the standard LM will likely begin to change. Advanced imaging involving both diffraction-limited and subdiffraction techniques are bringing nondestructive, high-resolution, molecular-level imaging to pathology. Some of these technologies can produce three-dimensional (3D) datasets from sampled tissues. In addition, block-face/tissue-sectioning techniques are already providing automated, large-scale 3D datasets of whole specimens. These datasets allow pathologists to see an entire sample with all of its spatial information intact, and furthermore allow image analysis such as detection, segmentation, and classification, which are impossible in standard LM. It is likely that these technologies herald a major paradigm shift in the field of pathology. |
format | Online Article Text |
id | pubmed-5027735 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-50277352016-09-29 The coming paradigm shift: A transition from manual to automated microscopy Farahani, Navid Monteith, Corey E. J Pathol Inform Editorial The field of pathology has used light microscopy (LM) extensively since the mid-19(th) century for examination of histological tissue preparations. This technology has remained the foremost tool in use by pathologists even as other fields have undergone a great change in recent years through new technologies. However, as new microscopy techniques are perfected and made available, this reliance on the standard LM will likely begin to change. Advanced imaging involving both diffraction-limited and subdiffraction techniques are bringing nondestructive, high-resolution, molecular-level imaging to pathology. Some of these technologies can produce three-dimensional (3D) datasets from sampled tissues. In addition, block-face/tissue-sectioning techniques are already providing automated, large-scale 3D datasets of whole specimens. These datasets allow pathologists to see an entire sample with all of its spatial information intact, and furthermore allow image analysis such as detection, segmentation, and classification, which are impossible in standard LM. It is likely that these technologies herald a major paradigm shift in the field of pathology. Medknow Publications & Media Pvt Ltd 2016-09-01 /pmc/articles/PMC5027735/ /pubmed/27688926 http://dx.doi.org/10.4103/2153-3539.189698 Text en Copyright: © 2016 Journal of Pathology Informatics http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. |
spellingShingle | Editorial Farahani, Navid Monteith, Corey E. The coming paradigm shift: A transition from manual to automated microscopy |
title | The coming paradigm shift: A transition from manual to automated microscopy |
title_full | The coming paradigm shift: A transition from manual to automated microscopy |
title_fullStr | The coming paradigm shift: A transition from manual to automated microscopy |
title_full_unstemmed | The coming paradigm shift: A transition from manual to automated microscopy |
title_short | The coming paradigm shift: A transition from manual to automated microscopy |
title_sort | coming paradigm shift: a transition from manual to automated microscopy |
topic | Editorial |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027735/ https://www.ncbi.nlm.nih.gov/pubmed/27688926 http://dx.doi.org/10.4103/2153-3539.189698 |
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