The coming paradigm shift: A transition from manual to automated microscopy

The field of pathology has used light microscopy (LM) extensively since the mid-19(th) century for examination of histological tissue preparations. This technology has remained the foremost tool in use by pathologists even as other fields have undergone a great change in recent years through new tec...

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Autores principales: Farahani, Navid, Monteith, Corey E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027735/
https://www.ncbi.nlm.nih.gov/pubmed/27688926
http://dx.doi.org/10.4103/2153-3539.189698
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author Farahani, Navid
Monteith, Corey E.
author_facet Farahani, Navid
Monteith, Corey E.
author_sort Farahani, Navid
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description The field of pathology has used light microscopy (LM) extensively since the mid-19(th) century for examination of histological tissue preparations. This technology has remained the foremost tool in use by pathologists even as other fields have undergone a great change in recent years through new technologies. However, as new microscopy techniques are perfected and made available, this reliance on the standard LM will likely begin to change. Advanced imaging involving both diffraction-limited and subdiffraction techniques are bringing nondestructive, high-resolution, molecular-level imaging to pathology. Some of these technologies can produce three-dimensional (3D) datasets from sampled tissues. In addition, block-face/tissue-sectioning techniques are already providing automated, large-scale 3D datasets of whole specimens. These datasets allow pathologists to see an entire sample with all of its spatial information intact, and furthermore allow image analysis such as detection, segmentation, and classification, which are impossible in standard LM. It is likely that these technologies herald a major paradigm shift in the field of pathology.
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spelling pubmed-50277352016-09-29 The coming paradigm shift: A transition from manual to automated microscopy Farahani, Navid Monteith, Corey E. J Pathol Inform Editorial The field of pathology has used light microscopy (LM) extensively since the mid-19(th) century for examination of histological tissue preparations. This technology has remained the foremost tool in use by pathologists even as other fields have undergone a great change in recent years through new technologies. However, as new microscopy techniques are perfected and made available, this reliance on the standard LM will likely begin to change. Advanced imaging involving both diffraction-limited and subdiffraction techniques are bringing nondestructive, high-resolution, molecular-level imaging to pathology. Some of these technologies can produce three-dimensional (3D) datasets from sampled tissues. In addition, block-face/tissue-sectioning techniques are already providing automated, large-scale 3D datasets of whole specimens. These datasets allow pathologists to see an entire sample with all of its spatial information intact, and furthermore allow image analysis such as detection, segmentation, and classification, which are impossible in standard LM. It is likely that these technologies herald a major paradigm shift in the field of pathology. Medknow Publications & Media Pvt Ltd 2016-09-01 /pmc/articles/PMC5027735/ /pubmed/27688926 http://dx.doi.org/10.4103/2153-3539.189698 Text en Copyright: © 2016 Journal of Pathology Informatics http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Editorial
Farahani, Navid
Monteith, Corey E.
The coming paradigm shift: A transition from manual to automated microscopy
title The coming paradigm shift: A transition from manual to automated microscopy
title_full The coming paradigm shift: A transition from manual to automated microscopy
title_fullStr The coming paradigm shift: A transition from manual to automated microscopy
title_full_unstemmed The coming paradigm shift: A transition from manual to automated microscopy
title_short The coming paradigm shift: A transition from manual to automated microscopy
title_sort coming paradigm shift: a transition from manual to automated microscopy
topic Editorial
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027735/
https://www.ncbi.nlm.nih.gov/pubmed/27688926
http://dx.doi.org/10.4103/2153-3539.189698
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