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Plasma Epstein–Barr virus and Hepatitis B virus in non-Hodgkin lymphomas: Two lymphotropic, potentially oncogenic, latently occurring DNA viruses
CONTEXT: There is a need to study potential infective etiologies in lymphomas. Lymphocyte-transforming viruses can directly infect lymphocytes, disrupt normal cell functions, and promote cell division. Epstein–Barr virus (EBV) is known to be associated with several lymphomas, especially Hodgkin lymp...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027786/ https://www.ncbi.nlm.nih.gov/pubmed/27688607 http://dx.doi.org/10.4103/0971-5851.190353 |
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author | Sinha, Mahua Rao, Clementina Rama Premalata, C. S. Shafiulla, Mohammed Lakshmaiah, K. C. Jacob, Linu Abraham Babu, Govind K. Viveka, B. K. Appaji, L. Subramanyam, Jayshree R. |
author_facet | Sinha, Mahua Rao, Clementina Rama Premalata, C. S. Shafiulla, Mohammed Lakshmaiah, K. C. Jacob, Linu Abraham Babu, Govind K. Viveka, B. K. Appaji, L. Subramanyam, Jayshree R. |
author_sort | Sinha, Mahua |
collection | PubMed |
description | CONTEXT: There is a need to study potential infective etiologies in lymphomas. Lymphocyte-transforming viruses can directly infect lymphocytes, disrupt normal cell functions, and promote cell division. Epstein–Barr virus (EBV) is known to be associated with several lymphomas, especially Hodgkin lymphomas (HLs). And recently, the lymphocyte-transforming role of hepatitis B virus (HBV) has been emphasized. AIMS: The aim of this study was to elucidate the association of two potentially oncogenic, widely prevalent latent DNA viruses, EBV and HBV, in non-HL (NHL). SETTINGS AND DESIGN: In this prospective study, we estimated plasma EBV and HBV DNA in NHL patients. MATERIALS AND METHODS: Peripheral blood was obtained from newly diagnosed, treatment na ïve, histologically confirmed NHL patients. Plasma EBV DNA was quantified by real-time polymerase chain reaction (PCR) targeting Epstein–Barr Nucleic acid 1 while the plasma HBV DNA was detected using nested PCR targeting HBX gene. In a small subset of patients, follow-up plasma samples post-anticancer chemotherapy were available and retested for viral DNA. RESULTS: Of the 110 NHL patients, ~79% were B-cell NHL and ~21% were T-cell NHL. Plasma EBV-DNA was detected in 10% NHLs with a higher EBV association in Burkitt lymphoma (33.3%) than other subtypes. Pretherapy HBV DNA was detected in 21% NHLs; most of them being diffuse large B-cell lymphoma (DLBCL). Moreover, 42% of DLBCL patients had HBV DNA in plasma. Since all patients were HBV surface antigen seronegative at diagnosis, baseline plasma HBV-DNAemia before chemotherapy was indicative of occult hepatitis B infection. CONCLUSIONS: Our findings indicate a significant association of HBV with newly diagnosed DLBCL. |
format | Online Article Text |
id | pubmed-5027786 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-50277862016-09-29 Plasma Epstein–Barr virus and Hepatitis B virus in non-Hodgkin lymphomas: Two lymphotropic, potentially oncogenic, latently occurring DNA viruses Sinha, Mahua Rao, Clementina Rama Premalata, C. S. Shafiulla, Mohammed Lakshmaiah, K. C. Jacob, Linu Abraham Babu, Govind K. Viveka, B. K. Appaji, L. Subramanyam, Jayshree R. Indian J Med Paediatr Oncol Original Article CONTEXT: There is a need to study potential infective etiologies in lymphomas. Lymphocyte-transforming viruses can directly infect lymphocytes, disrupt normal cell functions, and promote cell division. Epstein–Barr virus (EBV) is known to be associated with several lymphomas, especially Hodgkin lymphomas (HLs). And recently, the lymphocyte-transforming role of hepatitis B virus (HBV) has been emphasized. AIMS: The aim of this study was to elucidate the association of two potentially oncogenic, widely prevalent latent DNA viruses, EBV and HBV, in non-HL (NHL). SETTINGS AND DESIGN: In this prospective study, we estimated plasma EBV and HBV DNA in NHL patients. MATERIALS AND METHODS: Peripheral blood was obtained from newly diagnosed, treatment na ïve, histologically confirmed NHL patients. Plasma EBV DNA was quantified by real-time polymerase chain reaction (PCR) targeting Epstein–Barr Nucleic acid 1 while the plasma HBV DNA was detected using nested PCR targeting HBX gene. In a small subset of patients, follow-up plasma samples post-anticancer chemotherapy were available and retested for viral DNA. RESULTS: Of the 110 NHL patients, ~79% were B-cell NHL and ~21% were T-cell NHL. Plasma EBV-DNA was detected in 10% NHLs with a higher EBV association in Burkitt lymphoma (33.3%) than other subtypes. Pretherapy HBV DNA was detected in 21% NHLs; most of them being diffuse large B-cell lymphoma (DLBCL). Moreover, 42% of DLBCL patients had HBV DNA in plasma. Since all patients were HBV surface antigen seronegative at diagnosis, baseline plasma HBV-DNAemia before chemotherapy was indicative of occult hepatitis B infection. CONCLUSIONS: Our findings indicate a significant association of HBV with newly diagnosed DLBCL. Medknow Publications & Media Pvt Ltd 2016 /pmc/articles/PMC5027786/ /pubmed/27688607 http://dx.doi.org/10.4103/0971-5851.190353 Text en Copyright: © Indian Journal of Medical and Paediatric Oncology http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Sinha, Mahua Rao, Clementina Rama Premalata, C. S. Shafiulla, Mohammed Lakshmaiah, K. C. Jacob, Linu Abraham Babu, Govind K. Viveka, B. K. Appaji, L. Subramanyam, Jayshree R. Plasma Epstein–Barr virus and Hepatitis B virus in non-Hodgkin lymphomas: Two lymphotropic, potentially oncogenic, latently occurring DNA viruses |
title | Plasma Epstein–Barr virus and Hepatitis B virus in non-Hodgkin lymphomas: Two lymphotropic, potentially oncogenic, latently occurring DNA viruses |
title_full | Plasma Epstein–Barr virus and Hepatitis B virus in non-Hodgkin lymphomas: Two lymphotropic, potentially oncogenic, latently occurring DNA viruses |
title_fullStr | Plasma Epstein–Barr virus and Hepatitis B virus in non-Hodgkin lymphomas: Two lymphotropic, potentially oncogenic, latently occurring DNA viruses |
title_full_unstemmed | Plasma Epstein–Barr virus and Hepatitis B virus in non-Hodgkin lymphomas: Two lymphotropic, potentially oncogenic, latently occurring DNA viruses |
title_short | Plasma Epstein–Barr virus and Hepatitis B virus in non-Hodgkin lymphomas: Two lymphotropic, potentially oncogenic, latently occurring DNA viruses |
title_sort | plasma epstein–barr virus and hepatitis b virus in non-hodgkin lymphomas: two lymphotropic, potentially oncogenic, latently occurring dna viruses |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027786/ https://www.ncbi.nlm.nih.gov/pubmed/27688607 http://dx.doi.org/10.4103/0971-5851.190353 |
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