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Protein oxidation and degradation caused by particulate matter

Particulate matter (PM) modulates the expression of autophagy; however, the role of selective autophagy by PM remains unclear. The objective of this study was to determine the underlying mechanisms in protein oxidation and degradation caused by PM. Human epithelial A549 cells were exposed to diesel...

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Autores principales: Lai, Ching-Huang, Lee, Chun-Nin, Bai, Kuan-Jen, Yang, You-Lan, Chuang, Kai-Jen, Wu, Sheng-Ming, Chuang, Hsiao-Chi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5028717/
https://www.ncbi.nlm.nih.gov/pubmed/27644844
http://dx.doi.org/10.1038/srep33727
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author Lai, Ching-Huang
Lee, Chun-Nin
Bai, Kuan-Jen
Yang, You-Lan
Chuang, Kai-Jen
Wu, Sheng-Ming
Chuang, Hsiao-Chi
author_facet Lai, Ching-Huang
Lee, Chun-Nin
Bai, Kuan-Jen
Yang, You-Lan
Chuang, Kai-Jen
Wu, Sheng-Ming
Chuang, Hsiao-Chi
author_sort Lai, Ching-Huang
collection PubMed
description Particulate matter (PM) modulates the expression of autophagy; however, the role of selective autophagy by PM remains unclear. The objective of this study was to determine the underlying mechanisms in protein oxidation and degradation caused by PM. Human epithelial A549 cells were exposed to diesel exhaust particles (DEPs), urban dust (UD), and carbon black (CB; control particles). Cell survival and proliferation were significantly reduced by DEPs and UD in A549 cells. First, benzo(a)pyrene diolepoxide (BPDE) protein adduct was caused by DEPs at 150 μg/ml. Methionine oxidation (MetO) of human albumin proteins was induced by DEPs, UD, and CB; however, the protein repair mechanism that converts MetO back to methionine by methionine sulfoxide reductases A (MSRA) and B3 (MSRB3) was activated by DEPs and inhibited by UD, suggesting that oxidized protein was accumulating in cells. As to the degradation of oxidized proteins, proteasome and autophagy activation was induced by CB with ubiquitin accumulation, whereas proteasome and autophagy activation was induced by DEPs without ubiquitin accumulation. The results suggest that CB-induced protein degradation may be via an ubiquitin-dependent autophagy pathway, whereas DEP-induced protein degradation may be via an ubiquitin-independent autophagy pathway. A distinct proteotoxic effect may depend on the physicochemistry of PM.
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spelling pubmed-50287172016-09-26 Protein oxidation and degradation caused by particulate matter Lai, Ching-Huang Lee, Chun-Nin Bai, Kuan-Jen Yang, You-Lan Chuang, Kai-Jen Wu, Sheng-Ming Chuang, Hsiao-Chi Sci Rep Article Particulate matter (PM) modulates the expression of autophagy; however, the role of selective autophagy by PM remains unclear. The objective of this study was to determine the underlying mechanisms in protein oxidation and degradation caused by PM. Human epithelial A549 cells were exposed to diesel exhaust particles (DEPs), urban dust (UD), and carbon black (CB; control particles). Cell survival and proliferation were significantly reduced by DEPs and UD in A549 cells. First, benzo(a)pyrene diolepoxide (BPDE) protein adduct was caused by DEPs at 150 μg/ml. Methionine oxidation (MetO) of human albumin proteins was induced by DEPs, UD, and CB; however, the protein repair mechanism that converts MetO back to methionine by methionine sulfoxide reductases A (MSRA) and B3 (MSRB3) was activated by DEPs and inhibited by UD, suggesting that oxidized protein was accumulating in cells. As to the degradation of oxidized proteins, proteasome and autophagy activation was induced by CB with ubiquitin accumulation, whereas proteasome and autophagy activation was induced by DEPs without ubiquitin accumulation. The results suggest that CB-induced protein degradation may be via an ubiquitin-dependent autophagy pathway, whereas DEP-induced protein degradation may be via an ubiquitin-independent autophagy pathway. A distinct proteotoxic effect may depend on the physicochemistry of PM. Nature Publishing Group 2016-09-20 /pmc/articles/PMC5028717/ /pubmed/27644844 http://dx.doi.org/10.1038/srep33727 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Lai, Ching-Huang
Lee, Chun-Nin
Bai, Kuan-Jen
Yang, You-Lan
Chuang, Kai-Jen
Wu, Sheng-Ming
Chuang, Hsiao-Chi
Protein oxidation and degradation caused by particulate matter
title Protein oxidation and degradation caused by particulate matter
title_full Protein oxidation and degradation caused by particulate matter
title_fullStr Protein oxidation and degradation caused by particulate matter
title_full_unstemmed Protein oxidation and degradation caused by particulate matter
title_short Protein oxidation and degradation caused by particulate matter
title_sort protein oxidation and degradation caused by particulate matter
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5028717/
https://www.ncbi.nlm.nih.gov/pubmed/27644844
http://dx.doi.org/10.1038/srep33727
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