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Porcine milk-derived exosomes promote proliferation of intestinal epithelial cells
Milk-derived exosomes were identified as a novel mechanism of mother-to-child transmission of regulatory molecules, but their functions in intestinal tissues of neonates are not well-studied. Here, we characterized potential roles of porcine milk-derived exosomes in the intestinal tract. In vitro, t...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5028765/ https://www.ncbi.nlm.nih.gov/pubmed/27646050 http://dx.doi.org/10.1038/srep33862 |
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author | Chen, Ting Xie, Mei-Ying Sun, Jia-Jie Ye, Rui-Song Cheng, Xiao Sun, Rui-Ping Wei, Li-Min Li, Meng Lin, De-Lin Jiang, Qing-Yan Xi, Qian-Yun Zhang, Yong-Liang |
author_facet | Chen, Ting Xie, Mei-Ying Sun, Jia-Jie Ye, Rui-Song Cheng, Xiao Sun, Rui-Ping Wei, Li-Min Li, Meng Lin, De-Lin Jiang, Qing-Yan Xi, Qian-Yun Zhang, Yong-Liang |
author_sort | Chen, Ting |
collection | PubMed |
description | Milk-derived exosomes were identified as a novel mechanism of mother-to-child transmission of regulatory molecules, but their functions in intestinal tissues of neonates are not well-studied. Here, we characterized potential roles of porcine milk-derived exosomes in the intestinal tract. In vitro, treatment with milk-derived exosomes (27 ± 3 ng and 55 ± 5 ng total RNA) significantly promoted IPEC-J2 cell proliferation by MTT, CCK8, EdU fluorescence and EdU flow cytometry assays. The qRT-PCR and Western blot analyses indicated milk-derived exosomes (0.27 ± 0.03 μg total RNA) significantly promoted expression of CDX2, IGF-1R and PCNA, and inhibited p53 gene expression involved in intestinal proliferation. Additionally, six detected miRNAs were significantly increased in IPEC-J2 cell, while FAS and SERPINE were significantly down-regulated relative to that in control. In vivo, treated groups (0.125 μg and 0.25 μg total RNA) significantly raised mice’ villus height, crypt depth and ratio of villus length to crypt depth of intestinal tissues, significantly increased CDX2, PCNA and IGF-1R’ expression and significantly inhibited p53′ expression. Our study demonstrated that milk-derived exosomes can facilitate intestinal cell proliferation and intestinal tract development, thus giving a new insight for milk nutrition and newborn development and health. |
format | Online Article Text |
id | pubmed-5028765 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50287652016-09-26 Porcine milk-derived exosomes promote proliferation of intestinal epithelial cells Chen, Ting Xie, Mei-Ying Sun, Jia-Jie Ye, Rui-Song Cheng, Xiao Sun, Rui-Ping Wei, Li-Min Li, Meng Lin, De-Lin Jiang, Qing-Yan Xi, Qian-Yun Zhang, Yong-Liang Sci Rep Article Milk-derived exosomes were identified as a novel mechanism of mother-to-child transmission of regulatory molecules, but their functions in intestinal tissues of neonates are not well-studied. Here, we characterized potential roles of porcine milk-derived exosomes in the intestinal tract. In vitro, treatment with milk-derived exosomes (27 ± 3 ng and 55 ± 5 ng total RNA) significantly promoted IPEC-J2 cell proliferation by MTT, CCK8, EdU fluorescence and EdU flow cytometry assays. The qRT-PCR and Western blot analyses indicated milk-derived exosomes (0.27 ± 0.03 μg total RNA) significantly promoted expression of CDX2, IGF-1R and PCNA, and inhibited p53 gene expression involved in intestinal proliferation. Additionally, six detected miRNAs were significantly increased in IPEC-J2 cell, while FAS and SERPINE were significantly down-regulated relative to that in control. In vivo, treated groups (0.125 μg and 0.25 μg total RNA) significantly raised mice’ villus height, crypt depth and ratio of villus length to crypt depth of intestinal tissues, significantly increased CDX2, PCNA and IGF-1R’ expression and significantly inhibited p53′ expression. Our study demonstrated that milk-derived exosomes can facilitate intestinal cell proliferation and intestinal tract development, thus giving a new insight for milk nutrition and newborn development and health. Nature Publishing Group 2016-09-20 /pmc/articles/PMC5028765/ /pubmed/27646050 http://dx.doi.org/10.1038/srep33862 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Chen, Ting Xie, Mei-Ying Sun, Jia-Jie Ye, Rui-Song Cheng, Xiao Sun, Rui-Ping Wei, Li-Min Li, Meng Lin, De-Lin Jiang, Qing-Yan Xi, Qian-Yun Zhang, Yong-Liang Porcine milk-derived exosomes promote proliferation of intestinal epithelial cells |
title | Porcine milk-derived exosomes promote proliferation of intestinal epithelial cells |
title_full | Porcine milk-derived exosomes promote proliferation of intestinal epithelial cells |
title_fullStr | Porcine milk-derived exosomes promote proliferation of intestinal epithelial cells |
title_full_unstemmed | Porcine milk-derived exosomes promote proliferation of intestinal epithelial cells |
title_short | Porcine milk-derived exosomes promote proliferation of intestinal epithelial cells |
title_sort | porcine milk-derived exosomes promote proliferation of intestinal epithelial cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5028765/ https://www.ncbi.nlm.nih.gov/pubmed/27646050 http://dx.doi.org/10.1038/srep33862 |
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