Cargando…
Effects of Methotrexate in a Rabbit Model of In-Stent Neoatherosclerosis: An Optical Coherence Tomography Study
This study used optical coherence tomography (OCT) to investigate the effects of systemic methotrexate, in combination with a drug-eluting stent, on in-stent neoatherosclerosis in a rabbit model. Sirolimus-eluting stents were surgically implanted in the right common carotid arteries of 200 male New...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5028880/ https://www.ncbi.nlm.nih.gov/pubmed/27644847 http://dx.doi.org/10.1038/srep33657 |
_version_ | 1782454418494455808 |
---|---|
author | Zhang, Ruoxi Chen, Shuyuan Zhang, Hui Liu, Qi Xing, Jianpang Zhao, Qi Wang, Yu Yu, Bo Hou, Jingbo |
author_facet | Zhang, Ruoxi Chen, Shuyuan Zhang, Hui Liu, Qi Xing, Jianpang Zhao, Qi Wang, Yu Yu, Bo Hou, Jingbo |
author_sort | Zhang, Ruoxi |
collection | PubMed |
description | This study used optical coherence tomography (OCT) to investigate the effects of systemic methotrexate, in combination with a drug-eluting stent, on in-stent neoatherosclerosis in a rabbit model. Sirolimus-eluting stents were surgically implanted in the right common carotid arteries of 200 male New Zealand White rabbits; the animals received a high-fat diet, beginning one week before stent implantation. Each animal was randomly assigned to 1 of 4 groups, receiving intravenous injections of either methotrexate (0.4 mg/kg) or placebo weekly for 4 or 12 weeks. Stented arterial segments were harvested after stenting for 4 or 12 weeks, and processed for OCT and histological analysis. Prior to harvesting the arterial segments, blood was collected for the determinations of cytokine levels. Compared with the control animals, the methotrexate-treated animals showed lower rates of lipid-rich intima and per-strut low-signal intensity layers, smaller neointimal areas, and reduced neointimal thickness; larger fibrous cap thicknesses and smaller lumen areas were also seen in the animals receiving methotrexate. The levels of serum interleukin, adhesion molecules, and nuclear factor-κB p65 decreased and IL-10 level increased in the methotrexate-treated animals. Targeting the pro-inflammatory pathways may be an effective way to prevent restenosis without the long-term risk of late thrombosis. |
format | Online Article Text |
id | pubmed-5028880 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50288802016-09-26 Effects of Methotrexate in a Rabbit Model of In-Stent Neoatherosclerosis: An Optical Coherence Tomography Study Zhang, Ruoxi Chen, Shuyuan Zhang, Hui Liu, Qi Xing, Jianpang Zhao, Qi Wang, Yu Yu, Bo Hou, Jingbo Sci Rep Article This study used optical coherence tomography (OCT) to investigate the effects of systemic methotrexate, in combination with a drug-eluting stent, on in-stent neoatherosclerosis in a rabbit model. Sirolimus-eluting stents were surgically implanted in the right common carotid arteries of 200 male New Zealand White rabbits; the animals received a high-fat diet, beginning one week before stent implantation. Each animal was randomly assigned to 1 of 4 groups, receiving intravenous injections of either methotrexate (0.4 mg/kg) or placebo weekly for 4 or 12 weeks. Stented arterial segments were harvested after stenting for 4 or 12 weeks, and processed for OCT and histological analysis. Prior to harvesting the arterial segments, blood was collected for the determinations of cytokine levels. Compared with the control animals, the methotrexate-treated animals showed lower rates of lipid-rich intima and per-strut low-signal intensity layers, smaller neointimal areas, and reduced neointimal thickness; larger fibrous cap thicknesses and smaller lumen areas were also seen in the animals receiving methotrexate. The levels of serum interleukin, adhesion molecules, and nuclear factor-κB p65 decreased and IL-10 level increased in the methotrexate-treated animals. Targeting the pro-inflammatory pathways may be an effective way to prevent restenosis without the long-term risk of late thrombosis. Nature Publishing Group 2016-09-20 /pmc/articles/PMC5028880/ /pubmed/27644847 http://dx.doi.org/10.1038/srep33657 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Zhang, Ruoxi Chen, Shuyuan Zhang, Hui Liu, Qi Xing, Jianpang Zhao, Qi Wang, Yu Yu, Bo Hou, Jingbo Effects of Methotrexate in a Rabbit Model of In-Stent Neoatherosclerosis: An Optical Coherence Tomography Study |
title | Effects of Methotrexate in a Rabbit Model of In-Stent Neoatherosclerosis: An Optical Coherence Tomography Study |
title_full | Effects of Methotrexate in a Rabbit Model of In-Stent Neoatherosclerosis: An Optical Coherence Tomography Study |
title_fullStr | Effects of Methotrexate in a Rabbit Model of In-Stent Neoatherosclerosis: An Optical Coherence Tomography Study |
title_full_unstemmed | Effects of Methotrexate in a Rabbit Model of In-Stent Neoatherosclerosis: An Optical Coherence Tomography Study |
title_short | Effects of Methotrexate in a Rabbit Model of In-Stent Neoatherosclerosis: An Optical Coherence Tomography Study |
title_sort | effects of methotrexate in a rabbit model of in-stent neoatherosclerosis: an optical coherence tomography study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5028880/ https://www.ncbi.nlm.nih.gov/pubmed/27644847 http://dx.doi.org/10.1038/srep33657 |
work_keys_str_mv | AT zhangruoxi effectsofmethotrexateinarabbitmodelofinstentneoatherosclerosisanopticalcoherencetomographystudy AT chenshuyuan effectsofmethotrexateinarabbitmodelofinstentneoatherosclerosisanopticalcoherencetomographystudy AT zhanghui effectsofmethotrexateinarabbitmodelofinstentneoatherosclerosisanopticalcoherencetomographystudy AT liuqi effectsofmethotrexateinarabbitmodelofinstentneoatherosclerosisanopticalcoherencetomographystudy AT xingjianpang effectsofmethotrexateinarabbitmodelofinstentneoatherosclerosisanopticalcoherencetomographystudy AT zhaoqi effectsofmethotrexateinarabbitmodelofinstentneoatherosclerosisanopticalcoherencetomographystudy AT wangyu effectsofmethotrexateinarabbitmodelofinstentneoatherosclerosisanopticalcoherencetomographystudy AT yubo effectsofmethotrexateinarabbitmodelofinstentneoatherosclerosisanopticalcoherencetomographystudy AT houjingbo effectsofmethotrexateinarabbitmodelofinstentneoatherosclerosisanopticalcoherencetomographystudy |