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The prescriptions from Shenghui soup enhanced neurite growth and GAP-43 expression level in PC12 cells

BACKGROUND: Shenghui soup is a traditional Chinese herbal medicine used in clinic for the treatment of forgetfulness. In order to understanding the prescription principle, the effects of “tonifying qi and strengthening spleen” group (TQSS) including Poria cocos (Schw.) Wolf. and Panax ginseng C.A.Me...

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Autores principales: Zhang, Qi, Zhang, Zi-Jian, Wang, Xing-Hua, Ma, Jie, Song, Yue-Han, Liang, Mi, Lin, Sen-Xiang, Zhao, Jie, Zhang, Ao-Zhe, Li, Feng, Hua, Qian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5029060/
https://www.ncbi.nlm.nih.gov/pubmed/27646829
http://dx.doi.org/10.1186/s12906-016-1339-y
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author Zhang, Qi
Zhang, Zi-Jian
Wang, Xing-Hua
Ma, Jie
Song, Yue-Han
Liang, Mi
Lin, Sen-Xiang
Zhao, Jie
Zhang, Ao-Zhe
Li, Feng
Hua, Qian
author_facet Zhang, Qi
Zhang, Zi-Jian
Wang, Xing-Hua
Ma, Jie
Song, Yue-Han
Liang, Mi
Lin, Sen-Xiang
Zhao, Jie
Zhang, Ao-Zhe
Li, Feng
Hua, Qian
author_sort Zhang, Qi
collection PubMed
description BACKGROUND: Shenghui soup is a traditional Chinese herbal medicine used in clinic for the treatment of forgetfulness. In order to understanding the prescription principle, the effects of “tonifying qi and strengthening spleen” group (TQSS) including Poria cocos (Schw.) Wolf. and Panax ginseng C.A.Mey and “eliminating phlegm and strengthening intelligence” group (EPSI) composed of Polygala tenuifolia Willd., Acorus calamus L. and Sinapis alba L from the herb complex on neurite growth in PC12 cells, two disassembled prescriptions derived from Shenghui soup and their molecular mechanisms were investigated. METHODS: Firstly, CCK-8 kit was used to detect the impact of the two prescriptions on PC12 cell viability; and Flow cytometry was performed to measure the cell apoptosis when PC12 cells were treated with these drugs. Secondly, the effect of the two prescriptions on the differentiation of PC12 cells was observed. Finally, the mRNA and protein expression levels of GAP-43 were analyzed by RT-PCR and western blot, respectively. RESULTS: “Tonifying qi and strengthening spleen” prescription decreased cell viability in a dose-dependent manner, but had no significant effect on cell apoptosis. Meanwhile, it could improve neurite growth and elevate the mRNA and protein expression level of GAP-43. “Eliminating phlegm and strengthening intelligence” prescription also exerted the similar effects on cell viability and apoptosis. Furthermore, it could also enhance cell neurite growth, with a higher expression level of GAP-43 mRNA and protein. CONCLUSION: “Tonifying qi and strengthening spleen” and “eliminating phlegm and strengthening intelligence” prescriptions from Shenghui soup have a positive effect on neurite growth. Their effects are related to the up-regulating expression of GAP-43. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12906-016-1339-y) contains supplementary material, which is available to authorized users.
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spelling pubmed-50290602016-09-22 The prescriptions from Shenghui soup enhanced neurite growth and GAP-43 expression level in PC12 cells Zhang, Qi Zhang, Zi-Jian Wang, Xing-Hua Ma, Jie Song, Yue-Han Liang, Mi Lin, Sen-Xiang Zhao, Jie Zhang, Ao-Zhe Li, Feng Hua, Qian BMC Complement Altern Med Research Article BACKGROUND: Shenghui soup is a traditional Chinese herbal medicine used in clinic for the treatment of forgetfulness. In order to understanding the prescription principle, the effects of “tonifying qi and strengthening spleen” group (TQSS) including Poria cocos (Schw.) Wolf. and Panax ginseng C.A.Mey and “eliminating phlegm and strengthening intelligence” group (EPSI) composed of Polygala tenuifolia Willd., Acorus calamus L. and Sinapis alba L from the herb complex on neurite growth in PC12 cells, two disassembled prescriptions derived from Shenghui soup and their molecular mechanisms were investigated. METHODS: Firstly, CCK-8 kit was used to detect the impact of the two prescriptions on PC12 cell viability; and Flow cytometry was performed to measure the cell apoptosis when PC12 cells were treated with these drugs. Secondly, the effect of the two prescriptions on the differentiation of PC12 cells was observed. Finally, the mRNA and protein expression levels of GAP-43 were analyzed by RT-PCR and western blot, respectively. RESULTS: “Tonifying qi and strengthening spleen” prescription decreased cell viability in a dose-dependent manner, but had no significant effect on cell apoptosis. Meanwhile, it could improve neurite growth and elevate the mRNA and protein expression level of GAP-43. “Eliminating phlegm and strengthening intelligence” prescription also exerted the similar effects on cell viability and apoptosis. Furthermore, it could also enhance cell neurite growth, with a higher expression level of GAP-43 mRNA and protein. CONCLUSION: “Tonifying qi and strengthening spleen” and “eliminating phlegm and strengthening intelligence” prescriptions from Shenghui soup have a positive effect on neurite growth. Their effects are related to the up-regulating expression of GAP-43. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12906-016-1339-y) contains supplementary material, which is available to authorized users. BioMed Central 2016-09-20 /pmc/articles/PMC5029060/ /pubmed/27646829 http://dx.doi.org/10.1186/s12906-016-1339-y Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Zhang, Qi
Zhang, Zi-Jian
Wang, Xing-Hua
Ma, Jie
Song, Yue-Han
Liang, Mi
Lin, Sen-Xiang
Zhao, Jie
Zhang, Ao-Zhe
Li, Feng
Hua, Qian
The prescriptions from Shenghui soup enhanced neurite growth and GAP-43 expression level in PC12 cells
title The prescriptions from Shenghui soup enhanced neurite growth and GAP-43 expression level in PC12 cells
title_full The prescriptions from Shenghui soup enhanced neurite growth and GAP-43 expression level in PC12 cells
title_fullStr The prescriptions from Shenghui soup enhanced neurite growth and GAP-43 expression level in PC12 cells
title_full_unstemmed The prescriptions from Shenghui soup enhanced neurite growth and GAP-43 expression level in PC12 cells
title_short The prescriptions from Shenghui soup enhanced neurite growth and GAP-43 expression level in PC12 cells
title_sort prescriptions from shenghui soup enhanced neurite growth and gap-43 expression level in pc12 cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5029060/
https://www.ncbi.nlm.nih.gov/pubmed/27646829
http://dx.doi.org/10.1186/s12906-016-1339-y
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