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Rice NAC transcription factor ONAC095 plays opposite roles in drought and cold stress tolerance

BACKGROUND: The NAC (NAM, ATAF and CUC) transcriptional factors constitute a large family with more than 150 members in rice and some of them have been demonstrated to play crucial roles in plant abiotic stress response. Here, we report the characterization of a rice stress-responsive NAC gene, ONAC...

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Autores principales: Huang, Lei, Hong, Yongbo, Zhang, Huijuan, Li, Dayong, Song, Fengming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5029094/
https://www.ncbi.nlm.nih.gov/pubmed/27646344
http://dx.doi.org/10.1186/s12870-016-0897-y
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author Huang, Lei
Hong, Yongbo
Zhang, Huijuan
Li, Dayong
Song, Fengming
author_facet Huang, Lei
Hong, Yongbo
Zhang, Huijuan
Li, Dayong
Song, Fengming
author_sort Huang, Lei
collection PubMed
description BACKGROUND: The NAC (NAM, ATAF and CUC) transcriptional factors constitute a large family with more than 150 members in rice and some of them have been demonstrated to play crucial roles in plant abiotic stress response. Here, we report the characterization of a rice stress-responsive NAC gene, ONAC095, and the exploration of its function in drought and cold stress tolerance. RESULTS: Expression of ONAC095 was up-regulated by drought stress and abscisic acid (ABA) but down-regulated by cold stress. ONAC095 protein had transactivation activity and the C2 domain in C-terminal was found to be critical for transactivation activity. Transgenic rice lines with overexpression of ONAC095 (ONAC095-OE) and dominant chimeric repressor-mediated suppression of ONAC095 (ONAC095-SRDX) were generated. The ONAC095-OE plants showed comparable phenotype to wild type under drought and cold stress conditions. However, the ONAC095-SRDX plants displayed an improved drought tolerance but exhibited an attenuated cold tolerance. The ONAC095-SRDX plants had decreased water loss rate, increased proline and soluble sugar contents, and up-regulated expression of drought-responsive genes under drought condition, whereas the ONAC095-SRDX plants accumulated excess reactive oxygen species, increased malondialdehyde content and down-regulated expression of cold-responsive genes under cold condition. Furthermore, ONAC095-SRDX plants showed an increased ABA sensitivity, contained an elevated ABA level, and displayed altered expression of ABA biosynthetic and metabolic genes as well as some ABA signaling-related genes. CONCLUSION: Functional analyses through dominant chimeric repressor-mediated suppression of ONAC095 demonstrate that ONAC095 plays opposite roles in drought and cold stress tolerance, acting as a negative regulator of drought response but as a positive regulator of cold response in rice. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-016-0897-y) contains supplementary material, which is available to authorized users.
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spelling pubmed-50290942016-09-27 Rice NAC transcription factor ONAC095 plays opposite roles in drought and cold stress tolerance Huang, Lei Hong, Yongbo Zhang, Huijuan Li, Dayong Song, Fengming BMC Plant Biol Research Article BACKGROUND: The NAC (NAM, ATAF and CUC) transcriptional factors constitute a large family with more than 150 members in rice and some of them have been demonstrated to play crucial roles in plant abiotic stress response. Here, we report the characterization of a rice stress-responsive NAC gene, ONAC095, and the exploration of its function in drought and cold stress tolerance. RESULTS: Expression of ONAC095 was up-regulated by drought stress and abscisic acid (ABA) but down-regulated by cold stress. ONAC095 protein had transactivation activity and the C2 domain in C-terminal was found to be critical for transactivation activity. Transgenic rice lines with overexpression of ONAC095 (ONAC095-OE) and dominant chimeric repressor-mediated suppression of ONAC095 (ONAC095-SRDX) were generated. The ONAC095-OE plants showed comparable phenotype to wild type under drought and cold stress conditions. However, the ONAC095-SRDX plants displayed an improved drought tolerance but exhibited an attenuated cold tolerance. The ONAC095-SRDX plants had decreased water loss rate, increased proline and soluble sugar contents, and up-regulated expression of drought-responsive genes under drought condition, whereas the ONAC095-SRDX plants accumulated excess reactive oxygen species, increased malondialdehyde content and down-regulated expression of cold-responsive genes under cold condition. Furthermore, ONAC095-SRDX plants showed an increased ABA sensitivity, contained an elevated ABA level, and displayed altered expression of ABA biosynthetic and metabolic genes as well as some ABA signaling-related genes. CONCLUSION: Functional analyses through dominant chimeric repressor-mediated suppression of ONAC095 demonstrate that ONAC095 plays opposite roles in drought and cold stress tolerance, acting as a negative regulator of drought response but as a positive regulator of cold response in rice. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-016-0897-y) contains supplementary material, which is available to authorized users. BioMed Central 2016-09-20 /pmc/articles/PMC5029094/ /pubmed/27646344 http://dx.doi.org/10.1186/s12870-016-0897-y Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Huang, Lei
Hong, Yongbo
Zhang, Huijuan
Li, Dayong
Song, Fengming
Rice NAC transcription factor ONAC095 plays opposite roles in drought and cold stress tolerance
title Rice NAC transcription factor ONAC095 plays opposite roles in drought and cold stress tolerance
title_full Rice NAC transcription factor ONAC095 plays opposite roles in drought and cold stress tolerance
title_fullStr Rice NAC transcription factor ONAC095 plays opposite roles in drought and cold stress tolerance
title_full_unstemmed Rice NAC transcription factor ONAC095 plays opposite roles in drought and cold stress tolerance
title_short Rice NAC transcription factor ONAC095 plays opposite roles in drought and cold stress tolerance
title_sort rice nac transcription factor onac095 plays opposite roles in drought and cold stress tolerance
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5029094/
https://www.ncbi.nlm.nih.gov/pubmed/27646344
http://dx.doi.org/10.1186/s12870-016-0897-y
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