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Rapid evolution of microbe-mediated protection against pathogens in a worm host
Microbes can defend their host against virulent infections, but direct evidence for the adaptive origin of microbe-mediated protection is lacking. Using experimental evolution of a novel, tripartite interaction, we demonstrate that mildly pathogenic bacteria (Enterococcus faecalis) living in worms (...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5029159/ https://www.ncbi.nlm.nih.gov/pubmed/26978164 http://dx.doi.org/10.1038/ismej.2015.259 |
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author | King, Kayla C Brockhurst, Michael A Vasieva, Olga Paterson, Steve Betts, Alex Ford, Suzanne A Frost, Crystal L Horsburgh, Malcolm J Haldenby, Sam Hurst, Gregory DD |
author_facet | King, Kayla C Brockhurst, Michael A Vasieva, Olga Paterson, Steve Betts, Alex Ford, Suzanne A Frost, Crystal L Horsburgh, Malcolm J Haldenby, Sam Hurst, Gregory DD |
author_sort | King, Kayla C |
collection | PubMed |
description | Microbes can defend their host against virulent infections, but direct evidence for the adaptive origin of microbe-mediated protection is lacking. Using experimental evolution of a novel, tripartite interaction, we demonstrate that mildly pathogenic bacteria (Enterococcus faecalis) living in worms (Caenorhabditis elegans) rapidly evolved to defend their animal hosts against infection by a more virulent pathogen (Staphylococcus aureus), crossing the parasitism–mutualism continuum. Host protection evolved in all six, independently selected populations in response to within-host bacterial interactions and without direct selection for host health. Microbe-mediated protection was also effective against a broad spectrum of pathogenic S. aureus isolates. Genomic analysis implied that the mechanistic basis for E. faecalis-mediated protection was through increased production of antimicrobial superoxide, which was confirmed by biochemical assays. Our results indicate that microbes living within a host may make the evolutionary transition to mutualism in response to pathogen attack, and that microbiome evolution warrants consideration as a driver of infection outcome. |
format | Online Article Text |
id | pubmed-5029159 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50291592016-09-21 Rapid evolution of microbe-mediated protection against pathogens in a worm host King, Kayla C Brockhurst, Michael A Vasieva, Olga Paterson, Steve Betts, Alex Ford, Suzanne A Frost, Crystal L Horsburgh, Malcolm J Haldenby, Sam Hurst, Gregory DD ISME J Original Article Microbes can defend their host against virulent infections, but direct evidence for the adaptive origin of microbe-mediated protection is lacking. Using experimental evolution of a novel, tripartite interaction, we demonstrate that mildly pathogenic bacteria (Enterococcus faecalis) living in worms (Caenorhabditis elegans) rapidly evolved to defend their animal hosts against infection by a more virulent pathogen (Staphylococcus aureus), crossing the parasitism–mutualism continuum. Host protection evolved in all six, independently selected populations in response to within-host bacterial interactions and without direct selection for host health. Microbe-mediated protection was also effective against a broad spectrum of pathogenic S. aureus isolates. Genomic analysis implied that the mechanistic basis for E. faecalis-mediated protection was through increased production of antimicrobial superoxide, which was confirmed by biochemical assays. Our results indicate that microbes living within a host may make the evolutionary transition to mutualism in response to pathogen attack, and that microbiome evolution warrants consideration as a driver of infection outcome. Nature Publishing Group 2016-08 2016-03-15 /pmc/articles/PMC5029159/ /pubmed/26978164 http://dx.doi.org/10.1038/ismej.2015.259 Text en Copyright © 2016 International Society for Microbial Ecology http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Original Article King, Kayla C Brockhurst, Michael A Vasieva, Olga Paterson, Steve Betts, Alex Ford, Suzanne A Frost, Crystal L Horsburgh, Malcolm J Haldenby, Sam Hurst, Gregory DD Rapid evolution of microbe-mediated protection against pathogens in a worm host |
title | Rapid evolution of microbe-mediated protection against pathogens in a worm host |
title_full | Rapid evolution of microbe-mediated protection against pathogens in a worm host |
title_fullStr | Rapid evolution of microbe-mediated protection against pathogens in a worm host |
title_full_unstemmed | Rapid evolution of microbe-mediated protection against pathogens in a worm host |
title_short | Rapid evolution of microbe-mediated protection against pathogens in a worm host |
title_sort | rapid evolution of microbe-mediated protection against pathogens in a worm host |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5029159/ https://www.ncbi.nlm.nih.gov/pubmed/26978164 http://dx.doi.org/10.1038/ismej.2015.259 |
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