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3′READS+, a sensitive and accurate method for 3′ end sequencing of polyadenylated RNA
Sequencing of the 3′ end of poly(A)(+) RNA identifies cleavage and polyadenylation sites (pAs) and measures transcript expression. We previously developed a method, 3′ region extraction and deep sequencing (3′READS), to address mispriming issues that often plague 3′ end sequencing. Here we report a...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Cold Spring Harbor Laboratory Press
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5029459/ https://www.ncbi.nlm.nih.gov/pubmed/27512124 http://dx.doi.org/10.1261/rna.057075.116 |