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Cells must express components of the planar cell polarity system and extracellular matrix to support cytonemes

Drosophila dorsal air sac development depends on Decapentaplegic (Dpp) and Fibroblast growth factor (FGF) proteins produced by the wing imaginal disc and transported by cytonemes to the air sac primordium (ASP). Dpp and FGF signaling in the ASP was dependent on components of the planar cell polarity...

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Autores principales: Huang, Hai, Kornberg, Thomas B
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5030081/
https://www.ncbi.nlm.nih.gov/pubmed/27591355
http://dx.doi.org/10.7554/eLife.18979
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author Huang, Hai
Kornberg, Thomas B
author_facet Huang, Hai
Kornberg, Thomas B
author_sort Huang, Hai
collection PubMed
description Drosophila dorsal air sac development depends on Decapentaplegic (Dpp) and Fibroblast growth factor (FGF) proteins produced by the wing imaginal disc and transported by cytonemes to the air sac primordium (ASP). Dpp and FGF signaling in the ASP was dependent on components of the planar cell polarity (PCP) system in the disc, and neither Dpp- nor FGF-receiving cytonemes extended over mutant disc cells that lacked them. ASP cytonemes normally navigate through extracellular matrix (ECM) composed of collagen, laminin, Dally and Dally-like (Dlp) proteins that are stratified in layers over the disc cells. However, ECM over PCP mutant cells had reduced levels of laminin, Dally and Dlp, and whereas Dpp-receiving ASP cytonemes navigated in the Dally layer and required Dally (but not Dlp), FGF-receiving ASP cytonemes navigated in the Dlp layer, requiring Dlp (but not Dally). These findings suggest that cytonemes interact directly and specifically with proteins in the stratified ECM. DOI: http://dx.doi.org/10.7554/eLife.18979.001
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spelling pubmed-50300812016-09-21 Cells must express components of the planar cell polarity system and extracellular matrix to support cytonemes Huang, Hai Kornberg, Thomas B eLife Developmental Biology and Stem Cells Drosophila dorsal air sac development depends on Decapentaplegic (Dpp) and Fibroblast growth factor (FGF) proteins produced by the wing imaginal disc and transported by cytonemes to the air sac primordium (ASP). Dpp and FGF signaling in the ASP was dependent on components of the planar cell polarity (PCP) system in the disc, and neither Dpp- nor FGF-receiving cytonemes extended over mutant disc cells that lacked them. ASP cytonemes normally navigate through extracellular matrix (ECM) composed of collagen, laminin, Dally and Dally-like (Dlp) proteins that are stratified in layers over the disc cells. However, ECM over PCP mutant cells had reduced levels of laminin, Dally and Dlp, and whereas Dpp-receiving ASP cytonemes navigated in the Dally layer and required Dally (but not Dlp), FGF-receiving ASP cytonemes navigated in the Dlp layer, requiring Dlp (but not Dally). These findings suggest that cytonemes interact directly and specifically with proteins in the stratified ECM. DOI: http://dx.doi.org/10.7554/eLife.18979.001 eLife Sciences Publications, Ltd 2016-09-03 /pmc/articles/PMC5030081/ /pubmed/27591355 http://dx.doi.org/10.7554/eLife.18979 Text en © 2016, Huang et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology and Stem Cells
Huang, Hai
Kornberg, Thomas B
Cells must express components of the planar cell polarity system and extracellular matrix to support cytonemes
title Cells must express components of the planar cell polarity system and extracellular matrix to support cytonemes
title_full Cells must express components of the planar cell polarity system and extracellular matrix to support cytonemes
title_fullStr Cells must express components of the planar cell polarity system and extracellular matrix to support cytonemes
title_full_unstemmed Cells must express components of the planar cell polarity system and extracellular matrix to support cytonemes
title_short Cells must express components of the planar cell polarity system and extracellular matrix to support cytonemes
title_sort cells must express components of the planar cell polarity system and extracellular matrix to support cytonemes
topic Developmental Biology and Stem Cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5030081/
https://www.ncbi.nlm.nih.gov/pubmed/27591355
http://dx.doi.org/10.7554/eLife.18979
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