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Biostimulation of Indigenous Microbial Community for Bioremediation of Petroleum Refinery Sludge

Nutrient deficiency severely impairs the catabolic activity of indigenous microorganisms in hydrocarbon rich environments (HREs) and limits the rate of intrinsic bioremediation. The present study aimed to characterize the microbial community in refinery waste and evaluate the scope for biostimulatio...

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Autores principales: Sarkar, Jayeeta, Kazy, Sufia K., Gupta, Abhishek, Dutta, Avishek, Mohapatra, Balaram, Roy, Ajoy, Bera, Paramita, Mitra, Adinpunya, Sar, Pinaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5030240/
https://www.ncbi.nlm.nih.gov/pubmed/27708623
http://dx.doi.org/10.3389/fmicb.2016.01407
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author Sarkar, Jayeeta
Kazy, Sufia K.
Gupta, Abhishek
Dutta, Avishek
Mohapatra, Balaram
Roy, Ajoy
Bera, Paramita
Mitra, Adinpunya
Sar, Pinaki
author_facet Sarkar, Jayeeta
Kazy, Sufia K.
Gupta, Abhishek
Dutta, Avishek
Mohapatra, Balaram
Roy, Ajoy
Bera, Paramita
Mitra, Adinpunya
Sar, Pinaki
author_sort Sarkar, Jayeeta
collection PubMed
description Nutrient deficiency severely impairs the catabolic activity of indigenous microorganisms in hydrocarbon rich environments (HREs) and limits the rate of intrinsic bioremediation. The present study aimed to characterize the microbial community in refinery waste and evaluate the scope for biostimulation based in situ bioremediation. Samples recovered from the wastewater lagoon of Guwahati refinery revealed a hydrocarbon enriched [high total petroleum hydrocarbon (TPH)], oxygen-, moisture-limited, reducing environment. Intrinsic biodegradation ability of the indigenous microorganisms was enhanced significantly (>80% reduction in TPH by 90 days) with nitrate amendment. Preferred utilization of both higher- (>C30) and middle- chain (C20-30) length hydrocarbons were evident from GC-MS analysis. Denaturing gradient gel electrophoresis and community level physiological profiling analyses indicated distinct shift in community’s composition and metabolic abilities following nitrogen (N) amendment. High throughput deep sequencing of 16S rRNA gene showed that the native community was mainly composed of hydrocarbon degrading, syntrophic, methanogenic, nitrate/iron/sulfur reducing facultative anaerobic bacteria and archaebacteria, affiliated to γ- and δ-Proteobacteria and Euryarchaeota respectively. Genes for aerobic and anaerobic alkane metabolism (alkB and bssA), methanogenesis (mcrA), denitrification (nirS and narG) and N(2) fixation (nifH) were detected. Concomitant to hydrocarbon degradation, lowering of dissolve O(2) and increase in oxidation-reduction potential (ORP) marked with an enrichment of N(2) fixing, nitrate reducing aerobic/facultative anaerobic members [e.g., Azovibrio, Pseudoxanthomonas and Comamonadaceae members] was evident in N amended microcosm. This study highlighted that indigenous community of refinery sludge was intrinsically diverse, yet appreciable rate of in situ bioremediation could be achieved by supplying adequate N sources.
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spelling pubmed-50302402016-10-05 Biostimulation of Indigenous Microbial Community for Bioremediation of Petroleum Refinery Sludge Sarkar, Jayeeta Kazy, Sufia K. Gupta, Abhishek Dutta, Avishek Mohapatra, Balaram Roy, Ajoy Bera, Paramita Mitra, Adinpunya Sar, Pinaki Front Microbiol Microbiology Nutrient deficiency severely impairs the catabolic activity of indigenous microorganisms in hydrocarbon rich environments (HREs) and limits the rate of intrinsic bioremediation. The present study aimed to characterize the microbial community in refinery waste and evaluate the scope for biostimulation based in situ bioremediation. Samples recovered from the wastewater lagoon of Guwahati refinery revealed a hydrocarbon enriched [high total petroleum hydrocarbon (TPH)], oxygen-, moisture-limited, reducing environment. Intrinsic biodegradation ability of the indigenous microorganisms was enhanced significantly (>80% reduction in TPH by 90 days) with nitrate amendment. Preferred utilization of both higher- (>C30) and middle- chain (C20-30) length hydrocarbons were evident from GC-MS analysis. Denaturing gradient gel electrophoresis and community level physiological profiling analyses indicated distinct shift in community’s composition and metabolic abilities following nitrogen (N) amendment. High throughput deep sequencing of 16S rRNA gene showed that the native community was mainly composed of hydrocarbon degrading, syntrophic, methanogenic, nitrate/iron/sulfur reducing facultative anaerobic bacteria and archaebacteria, affiliated to γ- and δ-Proteobacteria and Euryarchaeota respectively. Genes for aerobic and anaerobic alkane metabolism (alkB and bssA), methanogenesis (mcrA), denitrification (nirS and narG) and N(2) fixation (nifH) were detected. Concomitant to hydrocarbon degradation, lowering of dissolve O(2) and increase in oxidation-reduction potential (ORP) marked with an enrichment of N(2) fixing, nitrate reducing aerobic/facultative anaerobic members [e.g., Azovibrio, Pseudoxanthomonas and Comamonadaceae members] was evident in N amended microcosm. This study highlighted that indigenous community of refinery sludge was intrinsically diverse, yet appreciable rate of in situ bioremediation could be achieved by supplying adequate N sources. Frontiers Media S.A. 2016-09-21 /pmc/articles/PMC5030240/ /pubmed/27708623 http://dx.doi.org/10.3389/fmicb.2016.01407 Text en Copyright © 2016 Sarkar, Kazy, Gupta, Dutta, Mohapatra, Roy, Bera, Mitra and Sar. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Sarkar, Jayeeta
Kazy, Sufia K.
Gupta, Abhishek
Dutta, Avishek
Mohapatra, Balaram
Roy, Ajoy
Bera, Paramita
Mitra, Adinpunya
Sar, Pinaki
Biostimulation of Indigenous Microbial Community for Bioremediation of Petroleum Refinery Sludge
title Biostimulation of Indigenous Microbial Community for Bioremediation of Petroleum Refinery Sludge
title_full Biostimulation of Indigenous Microbial Community for Bioremediation of Petroleum Refinery Sludge
title_fullStr Biostimulation of Indigenous Microbial Community for Bioremediation of Petroleum Refinery Sludge
title_full_unstemmed Biostimulation of Indigenous Microbial Community for Bioremediation of Petroleum Refinery Sludge
title_short Biostimulation of Indigenous Microbial Community for Bioremediation of Petroleum Refinery Sludge
title_sort biostimulation of indigenous microbial community for bioremediation of petroleum refinery sludge
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5030240/
https://www.ncbi.nlm.nih.gov/pubmed/27708623
http://dx.doi.org/10.3389/fmicb.2016.01407
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