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Localization of Components of the RNA-Degrading Machine in Bacillus subtilis
In bacteria, the control of mRNA stability is crucial to allow rapid adaptation to changing conditions. In most bacteria, RNA degradation is catalyzed by the RNA degradosome, a protein complex composed of endo- and exoribonucleases, RNA helicases, and accessory proteins. In the Gram-positive model o...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2016
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5030255/ https://www.ncbi.nlm.nih.gov/pubmed/27708634 http://dx.doi.org/10.3389/fmicb.2016.01492 |
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author | Cascante-Estepa, Nora Gunka, Katrin Stülke, Jörg |
author_facet | Cascante-Estepa, Nora Gunka, Katrin Stülke, Jörg |
author_sort | Cascante-Estepa, Nora |
collection | PubMed |
description | In bacteria, the control of mRNA stability is crucial to allow rapid adaptation to changing conditions. In most bacteria, RNA degradation is catalyzed by the RNA degradosome, a protein complex composed of endo- and exoribonucleases, RNA helicases, and accessory proteins. In the Gram-positive model organism Bacillus subtilis, the existence of a RNA degradosome assembled around the membrane-bound endoribonuclease RNase Y has been proposed. Here, we have studied the intracellular localization of the protein that have been implicated in the potential B. subtilis RNA degradosome, i.e., polynucleotide phosphorylase, the exoribonucleases J1 and J2, the DEAD-box RNA helicase CshA, and the glycolytic enzymes enolase and phosphofructokinase. Our data suggests that the bulk of these enzymes is located in the cytoplasm. The RNases J1 and J2 as well as the RNA helicase CshA were mainly localized in the peripheral regions of the cell where also the bulk of messenger RNA is localized. We were able to demonstrate active exclusion of these proteins from the transcribing nucleoid. Taken together, our findings suggest that the interactions of the enzymes involved in RNA degradation in B. subtilis are rather transient. |
format | Online Article Text |
id | pubmed-5030255 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-50302552016-10-05 Localization of Components of the RNA-Degrading Machine in Bacillus subtilis Cascante-Estepa, Nora Gunka, Katrin Stülke, Jörg Front Microbiol Microbiology In bacteria, the control of mRNA stability is crucial to allow rapid adaptation to changing conditions. In most bacteria, RNA degradation is catalyzed by the RNA degradosome, a protein complex composed of endo- and exoribonucleases, RNA helicases, and accessory proteins. In the Gram-positive model organism Bacillus subtilis, the existence of a RNA degradosome assembled around the membrane-bound endoribonuclease RNase Y has been proposed. Here, we have studied the intracellular localization of the protein that have been implicated in the potential B. subtilis RNA degradosome, i.e., polynucleotide phosphorylase, the exoribonucleases J1 and J2, the DEAD-box RNA helicase CshA, and the glycolytic enzymes enolase and phosphofructokinase. Our data suggests that the bulk of these enzymes is located in the cytoplasm. The RNases J1 and J2 as well as the RNA helicase CshA were mainly localized in the peripheral regions of the cell where also the bulk of messenger RNA is localized. We were able to demonstrate active exclusion of these proteins from the transcribing nucleoid. Taken together, our findings suggest that the interactions of the enzymes involved in RNA degradation in B. subtilis are rather transient. Frontiers Media S.A. 2016-09-21 /pmc/articles/PMC5030255/ /pubmed/27708634 http://dx.doi.org/10.3389/fmicb.2016.01492 Text en Copyright © 2016 Cascante-Estepa, Gunka and Stülke. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Cascante-Estepa, Nora Gunka, Katrin Stülke, Jörg Localization of Components of the RNA-Degrading Machine in Bacillus subtilis |
title | Localization of Components of the RNA-Degrading Machine in Bacillus subtilis |
title_full | Localization of Components of the RNA-Degrading Machine in Bacillus subtilis |
title_fullStr | Localization of Components of the RNA-Degrading Machine in Bacillus subtilis |
title_full_unstemmed | Localization of Components of the RNA-Degrading Machine in Bacillus subtilis |
title_short | Localization of Components of the RNA-Degrading Machine in Bacillus subtilis |
title_sort | localization of components of the rna-degrading machine in bacillus subtilis |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5030255/ https://www.ncbi.nlm.nih.gov/pubmed/27708634 http://dx.doi.org/10.3389/fmicb.2016.01492 |
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