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Dataset on gene expression profiling of multiple murine hair follicle populations
The murine hair follicle contains several different keratinocyte progenitor populations within its compartments. By using antibodies against CD34, Itgα6, Sca-1 and Plet-1, we have isolated eight populations and compared their Krt10 and Krt14 expressions using fluorescence microscopy. This improved p...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5030336/ https://www.ncbi.nlm.nih.gov/pubmed/27672671 http://dx.doi.org/10.1016/j.dib.2016.08.063 |
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author | Gunnarsson, Anders Patrik Christensen, Rikke Li, Jian Jensen, Uffe Birk |
author_facet | Gunnarsson, Anders Patrik Christensen, Rikke Li, Jian Jensen, Uffe Birk |
author_sort | Gunnarsson, Anders Patrik |
collection | PubMed |
description | The murine hair follicle contains several different keratinocyte progenitor populations within its compartments. By using antibodies against CD34, Itgα6, Sca-1 and Plet-1, we have isolated eight populations and compared their Krt10 and Krt14 expressions using fluorescence microscopy. This improved panel was used in our associated article doi:10.1016/j.scr.2016.06.002 (A.P. Gunnarsson, R. Christensen, J. Li, U.B. Jensen, 2016) [1] and the present dataset describes the basic controls for the FACS. We also used imaging flow cytometry to visualize the identified populations as control. A more detailed analysis of the global gene expression profiling is presented, focusing on the pilosebaceous unit. Murine whole-mounts were stained for heat shock protein Hspa2, which is exclusively expressed by keratinocytes with low or no expression of the four selection markers (IRK). Whole-mount labeling was also conducted to visualize Krt79 and Plet-1 coexpression within the hair follicle and quantification on the distribution of Krt79 positive keratinocytes is presented. |
format | Online Article Text |
id | pubmed-5030336 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-50303362016-09-26 Dataset on gene expression profiling of multiple murine hair follicle populations Gunnarsson, Anders Patrik Christensen, Rikke Li, Jian Jensen, Uffe Birk Data Brief Data Article The murine hair follicle contains several different keratinocyte progenitor populations within its compartments. By using antibodies against CD34, Itgα6, Sca-1 and Plet-1, we have isolated eight populations and compared their Krt10 and Krt14 expressions using fluorescence microscopy. This improved panel was used in our associated article doi:10.1016/j.scr.2016.06.002 (A.P. Gunnarsson, R. Christensen, J. Li, U.B. Jensen, 2016) [1] and the present dataset describes the basic controls for the FACS. We also used imaging flow cytometry to visualize the identified populations as control. A more detailed analysis of the global gene expression profiling is presented, focusing on the pilosebaceous unit. Murine whole-mounts were stained for heat shock protein Hspa2, which is exclusively expressed by keratinocytes with low or no expression of the four selection markers (IRK). Whole-mount labeling was also conducted to visualize Krt79 and Plet-1 coexpression within the hair follicle and quantification on the distribution of Krt79 positive keratinocytes is presented. Elsevier 2016-09-05 /pmc/articles/PMC5030336/ /pubmed/27672671 http://dx.doi.org/10.1016/j.dib.2016.08.063 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Data Article Gunnarsson, Anders Patrik Christensen, Rikke Li, Jian Jensen, Uffe Birk Dataset on gene expression profiling of multiple murine hair follicle populations |
title | Dataset on gene expression profiling of multiple murine hair follicle populations |
title_full | Dataset on gene expression profiling of multiple murine hair follicle populations |
title_fullStr | Dataset on gene expression profiling of multiple murine hair follicle populations |
title_full_unstemmed | Dataset on gene expression profiling of multiple murine hair follicle populations |
title_short | Dataset on gene expression profiling of multiple murine hair follicle populations |
title_sort | dataset on gene expression profiling of multiple murine hair follicle populations |
topic | Data Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5030336/ https://www.ncbi.nlm.nih.gov/pubmed/27672671 http://dx.doi.org/10.1016/j.dib.2016.08.063 |
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