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Biochemical and genome sequence analyses of Megasphaera sp. strain DISK18 from dental plaque of a healthy individual reveals commensal lifestyle

Much of the work in periodontal microbiology in recent years has focused on identifying and understanding periodontal pathogens. As the majority of oral microbes have not yet been isolated in pure form, it is essential to understand the phenotypic characteristics of microbes to decipher their role i...

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Autores principales: Nallabelli, Nayudu, Patil, Prashant P., Pal, Vijay Kumar, Singh, Namrata, Jain, Ashish, Patil, Prabhu B., Grover, Vishakha, Korpole, Suresh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5030485/
https://www.ncbi.nlm.nih.gov/pubmed/27651180
http://dx.doi.org/10.1038/srep33665
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author Nallabelli, Nayudu
Patil, Prashant P.
Pal, Vijay Kumar
Singh, Namrata
Jain, Ashish
Patil, Prabhu B.
Grover, Vishakha
Korpole, Suresh
author_facet Nallabelli, Nayudu
Patil, Prashant P.
Pal, Vijay Kumar
Singh, Namrata
Jain, Ashish
Patil, Prabhu B.
Grover, Vishakha
Korpole, Suresh
author_sort Nallabelli, Nayudu
collection PubMed
description Much of the work in periodontal microbiology in recent years has focused on identifying and understanding periodontal pathogens. As the majority of oral microbes have not yet been isolated in pure form, it is essential to understand the phenotypic characteristics of microbes to decipher their role in oral environment. In this study, strain DISK18 was isolated from gingival sulcus and identified as a Megasphaera species. Although metagenomics studies revealed Megasphaera species as a major group within the oral habitat, they have never been isolated in cultivable form to date. Therefore, we have characterized the DISK18 strain to better understand its role in the periodontal ecosystem. Strain Megasphaera sp. DISK18 displayed the ability to adhere and self-aggregate, which are essential requisite features for inhabiting and persisting in oral cavity. It also coaggregated with other pioneer oral colonizers like Streptococcus and Lactobacillus species but not with Veillonella. This behaviour points towards its role in the ecologic succession of a multispecies biofilm as an early colonizer. The absence of virulence determining genes as observed in whole genome sequence analysis coupled with an inability to degrade collagen reveals that Megasphaera sp. strain DISK18 is likely not a pathogenic species and emphasizes its commensal lifestyle.
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spelling pubmed-50304852016-09-26 Biochemical and genome sequence analyses of Megasphaera sp. strain DISK18 from dental plaque of a healthy individual reveals commensal lifestyle Nallabelli, Nayudu Patil, Prashant P. Pal, Vijay Kumar Singh, Namrata Jain, Ashish Patil, Prabhu B. Grover, Vishakha Korpole, Suresh Sci Rep Article Much of the work in periodontal microbiology in recent years has focused on identifying and understanding periodontal pathogens. As the majority of oral microbes have not yet been isolated in pure form, it is essential to understand the phenotypic characteristics of microbes to decipher their role in oral environment. In this study, strain DISK18 was isolated from gingival sulcus and identified as a Megasphaera species. Although metagenomics studies revealed Megasphaera species as a major group within the oral habitat, they have never been isolated in cultivable form to date. Therefore, we have characterized the DISK18 strain to better understand its role in the periodontal ecosystem. Strain Megasphaera sp. DISK18 displayed the ability to adhere and self-aggregate, which are essential requisite features for inhabiting and persisting in oral cavity. It also coaggregated with other pioneer oral colonizers like Streptococcus and Lactobacillus species but not with Veillonella. This behaviour points towards its role in the ecologic succession of a multispecies biofilm as an early colonizer. The absence of virulence determining genes as observed in whole genome sequence analysis coupled with an inability to degrade collagen reveals that Megasphaera sp. strain DISK18 is likely not a pathogenic species and emphasizes its commensal lifestyle. Nature Publishing Group 2016-09-21 /pmc/articles/PMC5030485/ /pubmed/27651180 http://dx.doi.org/10.1038/srep33665 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Nallabelli, Nayudu
Patil, Prashant P.
Pal, Vijay Kumar
Singh, Namrata
Jain, Ashish
Patil, Prabhu B.
Grover, Vishakha
Korpole, Suresh
Biochemical and genome sequence analyses of Megasphaera sp. strain DISK18 from dental plaque of a healthy individual reveals commensal lifestyle
title Biochemical and genome sequence analyses of Megasphaera sp. strain DISK18 from dental plaque of a healthy individual reveals commensal lifestyle
title_full Biochemical and genome sequence analyses of Megasphaera sp. strain DISK18 from dental plaque of a healthy individual reveals commensal lifestyle
title_fullStr Biochemical and genome sequence analyses of Megasphaera sp. strain DISK18 from dental plaque of a healthy individual reveals commensal lifestyle
title_full_unstemmed Biochemical and genome sequence analyses of Megasphaera sp. strain DISK18 from dental plaque of a healthy individual reveals commensal lifestyle
title_short Biochemical and genome sequence analyses of Megasphaera sp. strain DISK18 from dental plaque of a healthy individual reveals commensal lifestyle
title_sort biochemical and genome sequence analyses of megasphaera sp. strain disk18 from dental plaque of a healthy individual reveals commensal lifestyle
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5030485/
https://www.ncbi.nlm.nih.gov/pubmed/27651180
http://dx.doi.org/10.1038/srep33665
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