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The soluble pattern recognition receptor PTX3 links humoral innate and adaptive immune responses by helping marginal zone B cells
Pentraxin 3 (PTX3) is a fluid-phase pattern recognition receptor of the humoral innate immune system with ancestral antibody-like properties but unknown antibody-inducing function. In this study, we found binding of PTX3 to splenic marginal zone (MZ) B cells, an innate-like subset of antibody-produc...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5030794/ https://www.ncbi.nlm.nih.gov/pubmed/27621420 http://dx.doi.org/10.1084/jem.20150282 |
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author | Chorny, Alejo Casas-Recasens, Sandra Sintes, Jordi Shan, Meimei Polentarutti, Nadia García-Escudero, Ramón Walland, A. Cooper Yeiser, John R. Cassis, Linda Carrillo, Jorge Puga, Irene Cunha, Cristina Bastos, Hélder Rodrigues, Fernando Lacerda, João F. Morais, António Dieguez-Gonzalez, Rebeca Heeger, Peter S. Salvatori, Giovanni Carvalho, Agostinho Garcia-Sastre, Adolfo Blander, J. Magarian Mantovani, Alberto Garlanda, Cecilia Cerutti, Andrea |
author_facet | Chorny, Alejo Casas-Recasens, Sandra Sintes, Jordi Shan, Meimei Polentarutti, Nadia García-Escudero, Ramón Walland, A. Cooper Yeiser, John R. Cassis, Linda Carrillo, Jorge Puga, Irene Cunha, Cristina Bastos, Hélder Rodrigues, Fernando Lacerda, João F. Morais, António Dieguez-Gonzalez, Rebeca Heeger, Peter S. Salvatori, Giovanni Carvalho, Agostinho Garcia-Sastre, Adolfo Blander, J. Magarian Mantovani, Alberto Garlanda, Cecilia Cerutti, Andrea |
author_sort | Chorny, Alejo |
collection | PubMed |
description | Pentraxin 3 (PTX3) is a fluid-phase pattern recognition receptor of the humoral innate immune system with ancestral antibody-like properties but unknown antibody-inducing function. In this study, we found binding of PTX3 to splenic marginal zone (MZ) B cells, an innate-like subset of antibody-producing lymphocytes strategically positioned at the interface between the circulation and the adaptive immune system. PTX3 was released by a subset of neutrophils that surrounded the splenic MZ and expressed an immune activation–related gene signature distinct from that of circulating neutrophils. Binding of PTX3 promoted homeostatic production of IgM and class-switched IgG antibodies to microbial capsular polysaccharides, which decreased in PTX3-deficient mice and humans. In addition, PTX3 increased IgM and IgG production after infection with blood-borne encapsulated bacteria or immunization with bacterial carbohydrates. This immunogenic effect stemmed from the activation of MZ B cells through a neutrophil-regulated pathway that elicited class switching and plasmablast expansion via a combination of T cell–independent and T cell–dependent signals. Thus, PTX3 may bridge the humoral arms of the innate and adaptive immune systems by serving as an endogenous adjuvant for MZ B cells. This property could be harnessed to develop more effective vaccines against encapsulated pathogens. |
format | Online Article Text |
id | pubmed-5030794 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-50307942017-03-19 The soluble pattern recognition receptor PTX3 links humoral innate and adaptive immune responses by helping marginal zone B cells Chorny, Alejo Casas-Recasens, Sandra Sintes, Jordi Shan, Meimei Polentarutti, Nadia García-Escudero, Ramón Walland, A. Cooper Yeiser, John R. Cassis, Linda Carrillo, Jorge Puga, Irene Cunha, Cristina Bastos, Hélder Rodrigues, Fernando Lacerda, João F. Morais, António Dieguez-Gonzalez, Rebeca Heeger, Peter S. Salvatori, Giovanni Carvalho, Agostinho Garcia-Sastre, Adolfo Blander, J. Magarian Mantovani, Alberto Garlanda, Cecilia Cerutti, Andrea J Exp Med Research Articles Pentraxin 3 (PTX3) is a fluid-phase pattern recognition receptor of the humoral innate immune system with ancestral antibody-like properties but unknown antibody-inducing function. In this study, we found binding of PTX3 to splenic marginal zone (MZ) B cells, an innate-like subset of antibody-producing lymphocytes strategically positioned at the interface between the circulation and the adaptive immune system. PTX3 was released by a subset of neutrophils that surrounded the splenic MZ and expressed an immune activation–related gene signature distinct from that of circulating neutrophils. Binding of PTX3 promoted homeostatic production of IgM and class-switched IgG antibodies to microbial capsular polysaccharides, which decreased in PTX3-deficient mice and humans. In addition, PTX3 increased IgM and IgG production after infection with blood-borne encapsulated bacteria or immunization with bacterial carbohydrates. This immunogenic effect stemmed from the activation of MZ B cells through a neutrophil-regulated pathway that elicited class switching and plasmablast expansion via a combination of T cell–independent and T cell–dependent signals. Thus, PTX3 may bridge the humoral arms of the innate and adaptive immune systems by serving as an endogenous adjuvant for MZ B cells. This property could be harnessed to develop more effective vaccines against encapsulated pathogens. The Rockefeller University Press 2016-09-19 /pmc/articles/PMC5030794/ /pubmed/27621420 http://dx.doi.org/10.1084/jem.20150282 Text en © 2016 Chorny et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Chorny, Alejo Casas-Recasens, Sandra Sintes, Jordi Shan, Meimei Polentarutti, Nadia García-Escudero, Ramón Walland, A. Cooper Yeiser, John R. Cassis, Linda Carrillo, Jorge Puga, Irene Cunha, Cristina Bastos, Hélder Rodrigues, Fernando Lacerda, João F. Morais, António Dieguez-Gonzalez, Rebeca Heeger, Peter S. Salvatori, Giovanni Carvalho, Agostinho Garcia-Sastre, Adolfo Blander, J. Magarian Mantovani, Alberto Garlanda, Cecilia Cerutti, Andrea The soluble pattern recognition receptor PTX3 links humoral innate and adaptive immune responses by helping marginal zone B cells |
title | The soluble pattern recognition receptor PTX3 links humoral innate and adaptive immune responses by helping marginal zone B cells |
title_full | The soluble pattern recognition receptor PTX3 links humoral innate and adaptive immune responses by helping marginal zone B cells |
title_fullStr | The soluble pattern recognition receptor PTX3 links humoral innate and adaptive immune responses by helping marginal zone B cells |
title_full_unstemmed | The soluble pattern recognition receptor PTX3 links humoral innate and adaptive immune responses by helping marginal zone B cells |
title_short | The soluble pattern recognition receptor PTX3 links humoral innate and adaptive immune responses by helping marginal zone B cells |
title_sort | soluble pattern recognition receptor ptx3 links humoral innate and adaptive immune responses by helping marginal zone b cells |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5030794/ https://www.ncbi.nlm.nih.gov/pubmed/27621420 http://dx.doi.org/10.1084/jem.20150282 |
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