ddpcr: an R package and web application for analysis of droplet digital PCR data

Droplet digital polymerase chain reaction (ddPCR) is a novel platform for exact quantification of DNA which holds great promise in clinical diagnostics. It is increasingly popular due to its digital nature, which provides more accurate quantification and higher sensitivity than traditional real-time...

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Detalles Bibliográficos
Autores principales: Attali, Dean, Bidshahri, Roza, Haynes, Charles, Bryan, Jennifer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000Research 2016
Materias:
Software Tool Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5031129/
https://www.ncbi.nlm.nih.gov/pubmed/27703666
http://dx.doi.org/10.12688/f1000research.9022.1
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author Attali, Dean
Bidshahri, Roza
Haynes, Charles
Bryan, Jennifer
author_facet Attali, Dean
Bidshahri, Roza
Haynes, Charles
Bryan, Jennifer
author_sort Attali, Dean
collection PubMed
description Droplet digital polymerase chain reaction (ddPCR) is a novel platform for exact quantification of DNA which holds great promise in clinical diagnostics. It is increasingly popular due to its digital nature, which provides more accurate quantification and higher sensitivity than traditional real-time PCR. However, clinical adoption has been slowed in part by the lack of software tools available for analyzing ddPCR data. Here, we present ddpcr – a new R package for ddPCR visualization and analysis. In addition, ddpcr includes a web application (powered by the Shiny R package) that allows users to analyze ddPCR data using an interactive graphical interface.
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spelling pubmed-50311292016-10-03 ddpcr: an R package and web application for analysis of droplet digital PCR data Attali, Dean Bidshahri, Roza Haynes, Charles Bryan, Jennifer F1000Res Software Tool Article Droplet digital polymerase chain reaction (ddPCR) is a novel platform for exact quantification of DNA which holds great promise in clinical diagnostics. It is increasingly popular due to its digital nature, which provides more accurate quantification and higher sensitivity than traditional real-time PCR. However, clinical adoption has been slowed in part by the lack of software tools available for analyzing ddPCR data. Here, we present ddpcr – a new R package for ddPCR visualization and analysis. In addition, ddpcr includes a web application (powered by the Shiny R package) that allows users to analyze ddPCR data using an interactive graphical interface. F1000Research 2016-06-17 /pmc/articles/PMC5031129/ /pubmed/27703666 http://dx.doi.org/10.12688/f1000research.9022.1 Text en Copyright: © 2016 Attali D et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Software Tool Article
Attali, Dean
Bidshahri, Roza
Haynes, Charles
Bryan, Jennifer
ddpcr: an R package and web application for analysis of droplet digital PCR data
title ddpcr: an R package and web application for analysis of droplet digital PCR data
title_full ddpcr: an R package and web application for analysis of droplet digital PCR data
title_fullStr ddpcr: an R package and web application for analysis of droplet digital PCR data
title_full_unstemmed ddpcr: an R package and web application for analysis of droplet digital PCR data
title_short ddpcr: an R package and web application for analysis of droplet digital PCR data
title_sort ddpcr: an r package and web application for analysis of droplet digital pcr data
topic Software Tool Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5031129/
https://www.ncbi.nlm.nih.gov/pubmed/27703666
http://dx.doi.org/10.12688/f1000research.9022.1
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