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High-Efficiency Serum-Free Feeder-Free Erythroid Differentiation of Human Pluripotent Stem Cells Using Small Molecules
This article describes a good manufacturing practice (GMP)-compatible, feeder-free and serum-free method to produce large numbers of erythroid cells from human pluripotent stem cells (hPSCs), either embryonic or induced. This multistep protocol combines cytokines and small molecules to mimic and sur...
Autores principales: | Olivier, Emmanuel N., Marenah, Lamin, McCahill, Angela, Condie, Alison, Cowan, Scott, Mountford, Joanne C. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AlphaMed Press
2016
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5031182/ https://www.ncbi.nlm.nih.gov/pubmed/27400796 http://dx.doi.org/10.5966/sctm.2015-0371 |
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