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Preferential uptake of antioxidant carbon nanoparticles by T lymphocytes for immunomodulation
Autoimmune diseases mediated by a type of white blood cell—T lymphocytes—are currently treated using mainly broad-spectrum immunosuppressants that can lead to adverse side effects. Antioxidants represent an alternative approach for therapy of autoimmune disorders; however, dietary antioxidants are i...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5031970/ https://www.ncbi.nlm.nih.gov/pubmed/27654170 http://dx.doi.org/10.1038/srep33808 |
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author | Huq, Redwan Samuel, Errol L. G. Sikkema, William K. A. Nilewski, Lizanne G. Lee, Thomas Tanner, Mark R. Khan, Fatima S. Porter, Paul C. Tajhya, Rajeev B. Patel, Rutvik S. Inoue, Taeko Pautler, Robia G. Corry, David B. Tour, James M. Beeton, Christine |
author_facet | Huq, Redwan Samuel, Errol L. G. Sikkema, William K. A. Nilewski, Lizanne G. Lee, Thomas Tanner, Mark R. Khan, Fatima S. Porter, Paul C. Tajhya, Rajeev B. Patel, Rutvik S. Inoue, Taeko Pautler, Robia G. Corry, David B. Tour, James M. Beeton, Christine |
author_sort | Huq, Redwan |
collection | PubMed |
description | Autoimmune diseases mediated by a type of white blood cell—T lymphocytes—are currently treated using mainly broad-spectrum immunosuppressants that can lead to adverse side effects. Antioxidants represent an alternative approach for therapy of autoimmune disorders; however, dietary antioxidants are insufficient to play this role. Antioxidant carbon nanoparticles scavenge reactive oxygen species (ROS) with higher efficacy than dietary and endogenous antioxidants. Furthermore, the affinity of carbon nanoparticles for specific cell types represents an emerging tactic for cell-targeted therapy. Here, we report that nontoxic poly(ethylene glycol)-functionalized hydrophilic carbon clusters (PEG-HCCs), known scavengers of the ROS superoxide (O(2)(•−)) and hydroxyl radical, are preferentially internalized by T lymphocytes over other splenic immune cells. We use this selectivity to inhibit T cell activation without affecting major functions of macrophages, antigen-presenting cells that are crucial for T cell activation. We also demonstrate the in vivo effectiveness of PEG-HCCs in reducing T lymphocyte-mediated inflammation in delayed-type hypersensitivity and in experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis. Our results suggest the preferential targeting of PEG-HCCs to T lymphocytes as a novel approach for T lymphocyte immunomodulation in autoimmune diseases without affecting other immune cells. |
format | Online Article Text |
id | pubmed-5031970 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50319702016-09-29 Preferential uptake of antioxidant carbon nanoparticles by T lymphocytes for immunomodulation Huq, Redwan Samuel, Errol L. G. Sikkema, William K. A. Nilewski, Lizanne G. Lee, Thomas Tanner, Mark R. Khan, Fatima S. Porter, Paul C. Tajhya, Rajeev B. Patel, Rutvik S. Inoue, Taeko Pautler, Robia G. Corry, David B. Tour, James M. Beeton, Christine Sci Rep Article Autoimmune diseases mediated by a type of white blood cell—T lymphocytes—are currently treated using mainly broad-spectrum immunosuppressants that can lead to adverse side effects. Antioxidants represent an alternative approach for therapy of autoimmune disorders; however, dietary antioxidants are insufficient to play this role. Antioxidant carbon nanoparticles scavenge reactive oxygen species (ROS) with higher efficacy than dietary and endogenous antioxidants. Furthermore, the affinity of carbon nanoparticles for specific cell types represents an emerging tactic for cell-targeted therapy. Here, we report that nontoxic poly(ethylene glycol)-functionalized hydrophilic carbon clusters (PEG-HCCs), known scavengers of the ROS superoxide (O(2)(•−)) and hydroxyl radical, are preferentially internalized by T lymphocytes over other splenic immune cells. We use this selectivity to inhibit T cell activation without affecting major functions of macrophages, antigen-presenting cells that are crucial for T cell activation. We also demonstrate the in vivo effectiveness of PEG-HCCs in reducing T lymphocyte-mediated inflammation in delayed-type hypersensitivity and in experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis. Our results suggest the preferential targeting of PEG-HCCs to T lymphocytes as a novel approach for T lymphocyte immunomodulation in autoimmune diseases without affecting other immune cells. Nature Publishing Group 2016-09-22 /pmc/articles/PMC5031970/ /pubmed/27654170 http://dx.doi.org/10.1038/srep33808 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Huq, Redwan Samuel, Errol L. G. Sikkema, William K. A. Nilewski, Lizanne G. Lee, Thomas Tanner, Mark R. Khan, Fatima S. Porter, Paul C. Tajhya, Rajeev B. Patel, Rutvik S. Inoue, Taeko Pautler, Robia G. Corry, David B. Tour, James M. Beeton, Christine Preferential uptake of antioxidant carbon nanoparticles by T lymphocytes for immunomodulation |
title | Preferential uptake of antioxidant carbon nanoparticles by T lymphocytes for immunomodulation |
title_full | Preferential uptake of antioxidant carbon nanoparticles by T lymphocytes for immunomodulation |
title_fullStr | Preferential uptake of antioxidant carbon nanoparticles by T lymphocytes for immunomodulation |
title_full_unstemmed | Preferential uptake of antioxidant carbon nanoparticles by T lymphocytes for immunomodulation |
title_short | Preferential uptake of antioxidant carbon nanoparticles by T lymphocytes for immunomodulation |
title_sort | preferential uptake of antioxidant carbon nanoparticles by t lymphocytes for immunomodulation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5031970/ https://www.ncbi.nlm.nih.gov/pubmed/27654170 http://dx.doi.org/10.1038/srep33808 |
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