High throughput sequencing analysis of RNA libraries reveals the influences of initial library and PCR methods on SELEX efficiency

The systemic evolution of ligands by exponential enrichment (SELEX) technique is a powerful and effective aptamer-selection procedure. However, modifications to the process can dramatically improve selection efficiency and aptamer performance. For example, droplet digital PCR (ddPCR) has been recent...

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Detalles Bibliográficos
Autores principales: Takahashi, Mayumi, Wu, Xiwei, Ho, Michelle, Chomchan, Pritsana, Rossi, John J., Burnett, John C., Zhou, Jiehua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5031971/
https://www.ncbi.nlm.nih.gov/pubmed/27652575
http://dx.doi.org/10.1038/srep33697

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